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Plasmid#119858PurposeCmv d0 dPspCas13b-GGS-Sun no-fusion constructDepositorInsertdCas13b-GGS-Sun
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
p426*-SpSgH
Plasmid#136993PurposeHelper plasmid for SpCas9 gRNA cloning and expressionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterSNR52Available sinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
BCJJ345
Plasmid#117504PurposeYAO:Cas9:E9 Golden Gate Level 1 Position 2 reverseDepositorInsertBpiI:tagt:YAO:Cas9-3(intron):E9:ttgc:BpiI
UseCRISPRTagsExpressionPlantMutationPromoterAtYAOAvailable sinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMpGE010-gR082
Plasmid#188553PurposeBinary vector for CRISPR/Cas9 targeted to MpIGPD in Marchantia polymorpha (for Agrobacterium-mediated genetic transformation)DepositorInsertgR082
UseCRISPR; Entry vectorTagsExpressionMutationPromoterAvailable sinceMay 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCJH019_SC101_pLAC_Cas12c(4)_Kan
Plasmid#183071PurposeWild-type Cas12c_4 in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.DepositorInsertCas12c_4
UseTagsExpressionBacterialMutationWild-typePromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJW1311
Plasmid#61254PurposeTemplate for cloning sgRNA(F+E) to generate new PU6::sgRNAs by PCR-fusionDepositorInsertsgRNA(F+E) targeting Y61A9L9.1
UseCRISPR; Cloning templateTagsExpressionMutationPromoterAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pU6-Fn-crRNA
Plasmid#78958PurposeCloning vector for expression of FnCpf1 crRNA. It contains BsmB1 site for cloning.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceJune 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
DS-NMcas
Plasmid#48646PurposeBacterial N. meningitidis Cas9 (NM) + tracrRNA expression, cloDF13/spectinomycinDepositorInsertsCas9
NM tracrRNA
UseCRISPRTagsExpressionMutationPromoterNM tracrRNA promoter and proCAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
Integrin-αv Ectodomain M400C
Plasmid#159642PurposeContains integrin alpha v residues 1–960 with the M400C mutationDepositorInsertIntegrin alpha v ectodomain
UseTagsExpressionMammalianMutationM400CPromoterAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
p426*-LbSgH
Plasmid#136992PurposeHelper plasmid for LbCpf1 gRNA cloning and expressionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterSNR52Available sinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCJH021_SC101_pLAC_Cas12c2_Kan
Plasmid#183072PurposeWild-type Cas12c2 (Yan et al., 2019) in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.DepositorInsertCas12c2
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_ABEmaxNG‐NGA
Plasmid#187055PurposeABEmax-SpCas9-NGADepositorInsertABEmaxNG‐NGA
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pESR1-donor
Plasmid#112337PurposeCRISPR donor plasmid to tag human transcription factor ESR1 with GFPDepositorInsertESR1 homology arms flanking EGFP-IRES-Neo cassette (ESR1 Human)
UseCRISPRTagsExpressionMutationUnknownPromoterAvailable sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBSKΔB-TRE-dCas9-EGFP
Plasmid#62347PurposePlasmid for tet-inducible expression of dCas9-EGFP in mammalian cellsDepositorInsertdCas9-EGFP
UseCRISPRTagsHA and SV40 NLSExpressionMammalianMutationPromoterTREAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFOXF2-donor
Plasmid#113816PurposeCRISPR donor plasmid to tag human transcription factor FOXF2 with GFPDepositorInsertFOXF2 homology arms flanking EGFP-IRES-Neo cassette (FOXF2 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
M-SPcas
Plasmid#48668PurposeMammalian S. pyogenes Cas9 expression, human optimizedDepositorInsertCas9
UseCRISPRTagsNLSExpressionMutationPromoterCMVAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCRA03
Plasmid#103141PurposeTriple gRNA expression separated with 28nt Csy4 motifDepositorInsertgRNAs
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
DS-ST1cas
Plasmid#48647PurposeBacterial S. thermophilus #1 Cas9 (ST1) + tracrRNA expression, cloDF13/spectinomycinDepositorInsertsCas9
ST1 tracrRNA
UseCRISPRTagsExpressionMutationPromoterproCAvailable sinceNov. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFH55
Plasmid#125814PurposeLevel0 nSpCas9-PmCDA, Arabidopsis codon optimizedDepositorInsertnSpCas9-PmCDA, Arabidopsis codon optimized
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL198
Plasmid#180472PurposeFor expression of Cas9 and gRNAs, multiple gRNAs cloning vectorDepositorTypeEmpty backboneUseCRISPRTagsZmCas9ExpressionPlantMutationPromoterAvailable sinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
BCJJ180
Plasmid#117516PurposeCas9_4 Golden Gate Level 0DepositorInsertBsaI:AATG:Cas9_4:GCTT:BsaI
UseCRISPRTags3xFLAG and NLSExpressionPlantMutationPromoterAvailable sinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pxpr_050-wt-ltr
Plasmid#182646Purposelentiviral expression of gRNA scaffold, contains intact LTRDepositorInsertNone
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pICSL90016
Plasmid#117514PurposeCas9_2 Golden Gate Level 0DepositorInsertBsaI:AATG:Cas9_2:GCTT:BsaI
UseCRISPRTagsNLS_SV40ExpressionPlantMutationPromoterAvailable sinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pERF-donor
Plasmid#112299PurposeCRISPR donor plasmid to tag human transcription factor ERF with GFPDepositorInsertERF homology arms flanking EGFP-IRES-Neo cassette (ERF Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
BCJJ358
Plasmid#117505PurposeRPS5a:Cas9:E9 Golden Gate Level 1 Position 2 reverseDepositorInsertBpiI:tagt:RPS5a:Cas9_4:E9:ttgc:BpiI
UseCRISPRTagsExpressionPlantMutationPromoterAtRPS5aAvailable sinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMM705
Plasmid#68892PurposeIPTG-inducible CRISPRi vector targeting BT1754, pNBU2 backbone, AmpRDepositorInsertsdCas9
LacI
sgRNA
UseTagsExpressionMutationPromoterAvailable sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-U6-RNF103-CHMP3
Plasmid#140584PurposeExpresses a gRNA and mCherry in mammalian cellsDepositorInsertgRNA RNF103-CHMP3
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRVV661
Plasmid#108282PurposeDonor vector for creation of PhiC31 attP-flanked platforms for cassette exchange using CRISPR/Cas9DepositorInsertPhiC31 attP sites
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
Cas9m2-VP64
Plasmid#47317PurposeCas9m2 ActivatorDepositorInsertCas9m2-VP64
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pISL1-donor
Plasmid#113820PurposeCRISPR donor plasmid to tag human transcription factor ISL1 with GFPDepositorInsertISL1 homology arms flanking EGFP-IRES-Neo cassette (ISL1 Human)
UseCRISPRTagsExpressionMutationUnknownPromoterAvailable sinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
library backbone 3'LTR gRNA and barcode
Plasmid#127397PurposeDigestion by BstXI and BamHI to release the backboneDepositorTypeEmpty backboneUseLentiviralTagsPuro-mCherryExpressionMutationPromoterAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH23
Plasmid#128180PurposeLevel1 ZmUBIp::SpCas9 (wheat codon optimized):NOStDepositorInsertZmUBIp::SpCas9 (wheat codon optimized):NOSt
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH53
Plasmid#128182PurposeLevel1 2x35Sp::SpCas9 (Arabidopsis codon optimized):pea3AtDepositorInsert2x35Sp::SpCas9 (Arabidopsis codon optimized):pea3At
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMLS546
Plasmid#188883PurposeIntegrating Pmex-5::Cas9 expression vectorDepositorInsertPmex-5::Cas9
UseTagsExpressionWormMutationC. elegans codom optimizationPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFH58
Plasmid#128184PurposeLevel1 ZmUBIp::SaCas9 (Arabidopsis codon optimized):NOStDepositorInsertZmUBIp::SaCas9 (Arabidopsis codon optimized):NOSt
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH24
Plasmid#131763PurposeLevel0 SpCas9, wheat codon optimized (version 2)DepositorInsertSpCas9, wheat codon optimized, version 2
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH92
Plasmid#125820PurposeLevel0 nScCas9-ABE7.10, wheat/human codon optimizedDepositorInsertnScCas9-ABE7.10, wheat/human codon optimized
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCF711
Plasmid#121662PurposeU6-sgRNA EFS-ProCas9-FlaviS6-P2A-PuroDepositorInsertsUseLentiviralTagsExpressionMutationPromoterU6Available sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
C-12-BE
Plasmid#135040PurposeC-T base editing using LjCDA1L2_1 fused to C terminal of nCas9DepositorInsertLjCDA1L2_1
UseCRISPRTagsExpressionMammalianMutationPromoterU6 with EF-1alpha intronAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
N-8-BE
Plasmid#135023PurposeC-T base editing using LpCDA1L1_1 fused to N terminal of nCas9DepositorInsertLpCDA1L1_1
UseCRISPRTagsExpressionMammalianMutationPromoterU6 with EF-1alpha intronAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFH52
Plasmid#128181PurposeLevel1 2x35Sp::SpCas9 (Arabidopsis codon optimized):NOStDepositorInsert2x35Sp::SpCas9 (Arabidopsis codon optimized):NOSt
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCF713
Plasmid#121665PurposeU6-sgRNA EF1a-ProCas9-FlaviS6-P2A-PuroDepositorInsertsUseLentiviralTagsExpressionMutationPromoterU6Available sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cas9m3-VP64
Plasmid#47318PurposeCas9m3 ActivatorDepositorInsertCas9m3-VP64
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
C-1-BE
Plasmid#135029PurposeC-T base editing using PmCDA1L1_4 fused to C terminal of nCas9DepositorInsertPmCDA1L1_4
UseCRISPRTagsExpressionMammalianMutationPromoterU6 with EF-1alpha intronAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-huPcCas12a
Plasmid#174681PurposeExpresses 6xHis-MBP tagged PcCas12aDepositorArticleInserthuPcCas12a
UseCRISPR and Synthetic BiologyTags6xHis, MBP, TEV and NLS, 6xHis, 3xHAExpressionBacterialMutationPromoterT7Available sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-huPdCas12a
Plasmid#174682PurposeExpresses 6xHis-MBP tagged PdCas12aDepositorArticleInserthuPdCas12a
UseCRISPR and Synthetic BiologyTags6xHis, MBP, TEV and NLS, 6xHis, 3xHAExpressionBacterialMutationPromoterT7Available sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMLX-donor
Plasmid#112370PurposeCRISPR donor plasmid to tag human transcription factor MLX with GFPDepositorInsertMLX homology arms flanking EGFP-IRES-Neo cassette (MLX Human)
UseCRISPRTagsExpressionMutationhg38:17:42570798:G>A, hg38:17:42570950:T>G,…PromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHOXB13-donor
Plasmid#113814PurposeCRISPR donor plasmid to tag human transcription factor HOXB13 with GFPDepositorInsertHOXB13 homology arms flanking EGFP-IRES-Neo cassette (HOXB13 Human)
UseCRISPRTagsExpressionMutationrs139887310PromoterAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
N-4-BE
Plasmid#135019PurposeC-T base editing using LjCDA1L1_4 fused to N terminal of nCas9DepositorInsertLjCDA1L1_4
UseCRISPRTagsExpressionMammalianMutationPromoterU6 with EF-1alpha intronAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRVV584
Plasmid#108280PurposeDonor vector for creation of PhiC31 attP-flanked platforms for cassette exchange using CRISPR/Cas9DepositorInsertPhiC31 attP sites
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only