We narrowed to 13,725 results for: cas9

1. pSEVA6213S

   Plasmid

   #122094

   Purpose

   constitutive expression of I-SceI for genome engineering in Pseudomonas

   Depositor

   Pablo Ivan Nikel

   Article

   Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

   Insert

   I-SceI restriction endonuclease
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Pem7

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCF525-AcrIIA4_gb39_lenti

   Plasmid

   #138305

   Purpose

   Lenti expression of AcrIIA4

   Depositor

   Jennifer Doudna

   Article

   Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

   Insert

   AcrIIA4
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGNW2

   Plasmid

   #122086

   Purpose

   suicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cells

   Depositor

   Pablo Ivan Nikel

   Article

   Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330_SYCP3_nterm

   Plasmid

   #222524

   Purpose

   Cas9/sgRNA plasmid for targeting SYCP3

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2024.05.31.596483

   Insert

   Cas9, SYCP3 sgRNA (SYCP3 Human, Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJAT10

   Plasmid

   #204293

   Purpose

   For marking CRISPR HDR integrant with DsRed expressed in flight muscles.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   MHC-DsRed
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   MHC

   Available since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYTK-DN4

   Plasmid

   #180285

   Purpose

   KanR multi-gene yeast expression vector (GFP-dropout)

   Depositor

   Andreas Milias-Argeitis

   Article

   Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

   Insert

   ConLS' - GFP - ConRE' - KanR - CEN6/ARS4
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJAT32

   Plasmid

   #204297

   Purpose

   pJAT plasmid with multiple cloning site for introducing custom payload. Two U6 promoters.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFUGW NLS-FlpO

   Plasmid

   #183448

   Purpose

   Lentiviral FlpO vector

   Depositor

   Harold MacGillavry

   Article

   Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

   Insert

   NLS-FlpO
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRAU168

   Plasmid

   #86834

   Purpose

   bacterial expression of anti-CRISPR protein AcrIIA1

   Depositor

   Joseph Bondy-Denomy

   Article

   Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

   Insert

   acrIIA1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBFv-U6.2B

    Plasmid

    #138401

    Purpose

    gRNA expression vector; Can be combined with pBFv-U6.2 to generate double-gRNA expression vector

    Depositor

    Shu Kondo

    Article

    Kondo et al Genetics. 2013 Nov;195(3):715-21. doi: 1

    Insert

    U6-2
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6-2

    Available since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBGK

    Plasmid

    #65796

    Purpose

    Vector for Cas9 mRNA synthesis

    Depositor

    Masato Ohtsuka

    Article

    Harms et al Curr Protoc Hum Genet. 2014 Oct 1;83:15.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLH02

    Plasmid

    #117262

    Purpose

    Genome editing for Lactobacillus brevis ATCC367

    Depositor

    Sheng Yang

    Article

    Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

    Insert

    PorfX-LVISKB_1732-30 , sapK-sappR
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJAT39

    Plasmid

    #204289

    Purpose

    For marking CRISPR HDR integrant with EGFP expressed in flight muscles.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    MHC-EGFP+H3:H26
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    MHC

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJH375

    Plasmid

    #86841

    Purpose

    mamalian expression of anti-CRISPR protein AcrIIA3

    Depositor

    Joseph Bondy-Denomy

    Article

    Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

    Insert

    acrIIA3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL3-Basic-8x-gRNA-eGFP

    Plasmid

    #60718

    Purpose

    eGFP reporter containing 8 copies of a gRNA binding site for light-inducible dCas9 activation

    Depositor

    Charles Gersbach

    Article

    Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

    Insert

    8 copies of gRNA binding site (5'-AAAGGTCGAGAAACTGCAAA-3')
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. BFP dest clone

    Plasmid

    #71825

    Purpose

    Lentiviral introduction of BFP reporter (Point mutation H66 in mEGFP results in BFP expression)

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGNW4

    Plasmid

    #122088

    Purpose

    suicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cells

    Depositor

    Pablo Ivan Nikel

    Article

    Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. U6>Control(F+E)

    Plasmid

    #60006

    Purpose

    U6 promoter driving control (F+E) sgRNA

    Depositor

    Lionel Christiaen

    Article

    Stolfi et al Development. 2014 Nov;141(21):4115-20. d

    Insert

    Control (F+E) sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ciinte.U6

    Available since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-Cry2FL-VP64

    Plasmid

    #60554

    Purpose

    Expresses Cry2FL-VP64 in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

    Insert

    Cry2FL-VP64
    

    Use

    Tags

    FLAG, HA tag, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. YN2_1_LT58_X2site

    Plasmid

    #177705

    Purpose

    Expresses Cas9 and sgRNA cassette for targeting site X2 in yeast Saccharomyces cerevisiae

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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