We narrowed to 10,385 results for: plasmids 101

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miR21-IRE-TREAT

Plasmid

#196930

Purpose

mir21 binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT reporter.

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Expression

Bacterial and Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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Let7-IRE-TREAT

Plasmid

#196925

Purpose

Let7-miRNA binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT reporter.

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Expression

Bacterial and Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-Cas9-P2A-Puro_NFATC2-sgRNA

Plasmid

#188704

Purpose

Lentivirus transfer plasmid encoding Cas9, puromycin resistance, and 4 sgRNAs targeting human NFATC2

Depositor

David Tobin

Article

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Insert

NFATC2 sgRNAs

Use

Lentiviral

Expression

Mammalian

Promoter

hUbC

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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IRE-TPI-wildtype-TREAT

Plasmid

#196923

Purpose

The coding sequence of Triose phosphate isomerase gene was fused downstream Renilla in the ORF

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Expression

Bacterial and Mammalian

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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Let7R-IRE-TREAT

Plasmid

#196934

Purpose

Reverse complement of let7-miRNA binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT.

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Expression

Bacterial and Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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CAG-EGFP-Otx2shRNA2

Plasmid

#73982

Purpose

Otx2 shRNA2 (based on mir155) was inserted into the 3'UTR of EGFP.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

EGFP-Otx2-shRNA2

Use

RNAi

Expression

Mammalian

Promoter

CAG

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pKM149

Plasmid

#134178

Purpose

Bacterial expression of his-CENP-C 510-end

Depositor

Iain Cheeseman

Article

McKinley et al Cell. 2014 Jul 17;158(2):397-411. doi: 1

Insert

his-CENP-C 510-end (CENPC Human)

Expression

Bacterial

Mutation

aa 510-end

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pSR35

Plasmid

#69256

Purpose

phsp16.41::CRE for integration on ttTi14024, Chr X

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Insert

Cre recombinase

Use

Cre/Lox; Mossci

Expression

Worm

Mutation

codon-optimzed index 1.0

Promoter

hsp16.41

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO/intron/FlagCter

Plasmid

#113548

Purpose

Mammalian expression plasmid that can be used with the Flp-In T-REx system.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

Flag

Tags

Flag

Expression

Mammalian

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pSR28

Plasmid

#69253

Purpose

phlh-8::CRE for integration on ttTi14024, Chr X

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Insert

Cre recombinase

Use

Cre/Lox; Mossci

Expression

Worm

Mutation

codon-optimzed index 1.0

Promoter

hlh-8

Available Since

Oct. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pUC_h7SK Mma PylT (UUA)

Plasmid

#154783

Purpose

plasmid with single copy of Mma PylT (UUA anticodon)

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

Mma PylT

Expression

Mammalian

Mutation

U25C and UUA anticodon

Promoter

h7SK

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a>WAChR_1s-G-mScarlet-Soma-E; WPRE3-BGH polyA; AmpR

Plasmid

#247725

Purpose

Constitutive expression of WAChR-1s opsin fusion protein: {WAChR-1s}-{Kir2.1 Golgi tag}-{mScarlet}-{Kv2.1 soma tag}-{Kir2.1 ER tag}

Depositor

Alexander Naka

Article

Tose et al bioRxiv 2025.09.12.675947; doi: https://

Insert

WAChR_1s-G-mScarlet-Soma-E

Use

AAV

Tags

mScarlet

Expression

Mammalian

Mutation

F211W/F240A

Promoter

EF1a

Available Since

Dec. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a>WAChR_1f-G-mScarlet3-Soma-E; WPRE3-BGH polyA; AmpR

Plasmid

#247723

Purpose

Constitutive expression of WAChR-1f opsin fusion protein: {WAChR-1f}-{Kir2.1 Golgi tag}-{mScarlet3}-{Kv2.1 soma tag}-{Kir2.1 ER tag}

Depositor

Alexander Naka

Article

Tose et al bioRxiv 2025.09.12.675947; doi: https://

Insert

WAChR_1f-G-mScarlet3-Soma-E

Use

AAV

Tags

mScarlet3

Expression

Mammalian

Mutation

L130F/F240A

Promoter

EF1a

Available Since

Dec. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a>WAChR_1m-G-mScarlet-Soma-E; WPRE3-BGH polyA; AmpR

Plasmid

#247724

Purpose

Constitutive expression of WAChR-1m opsin fusion protein: {WAChR-1m}-{Kir2.1 Golgi tag}-{mScarlet}-{Kv2.1 soma tag}-{Kir2.1 ER tag}

Depositor

Alexander Naka

Article

Tose et al bioRxiv 2025.09.12.675947; doi: https://

Insert

WAChR_1m-G-mScarlet-Soma-E

Use

AAV

Tags

mScarlet

Expression

Mammalian

Mutation

S122L/F240A

Promoter

EF1a

Available Since

Dec. 19, 2025

Availability

Academic Institutions and Nonprofits only

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hSyn-PreSynBar-hSyn-PreRNA short

Plasmid

#247715

Purpose

SynBar protein and RNA construct used in HEK and neuron culture experiment.

Depositor

Boxuan Zhao

Article

Chen et al bioRxiv [Preprint]. 2025 May 26:2025.02.

Insert

PreSynBar and PreRNA short

Use

AAV

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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hSyn-PostSynBar-hSyn-PostRNA short

Plasmid

#247716

Purpose

SynBar protein and RNA construct used in HEK and neuron culture experiment.

Depositor

Boxuan Zhao

Article

Chen et al bioRxiv [Preprint]. 2025 May 26:2025.02.

Insert

PostSynBar and PostRNA short

Use

AAV

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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hSyn-PreSynBar-hSyn-PreRNA barcode landing pad

Plasmid

#247717

Purpose

The set of SynBar constructs used in the optimized connectome-seq experiments

Depositor

Boxuan Zhao

Article

Chen et al bioRxiv [Preprint]. 2025 May 26:2025.02.

Insert

PreSynBar and PreRNA

Use

AAV

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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HP17 sfGFP::P8

Plasmid

#235448

Purpose

Helper plasmid with sfGFP gene inserted at gene VIII locus

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

Coding part of the M13KO7 helper phage genome, except gene VIII

Expression

Bacterial

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19_PHF1_HTC3xF_BSD_donor

Plasmid

#246394

Purpose

PHF1 donor plasmid with blasticidin resistance

Depositor

Yicheng Long

Article

Much et al Mol Cell. 2025 Aug 7:S1097-2765(25)00615

Insert

PHF1 (PHF1 Human)

Tags

HaloTag_3XFLAG

Expression

Mammalian

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19_PHF1_HTC3xF_Puro_donor

Plasmid

#246395

Purpose

PHF1 donor plasmid with puromycin resistance

Depositor

Yicheng Long

Article

Much et al Mol Cell. 2025 Aug 7:S1097-2765(25)00615

Insert

PHF1 (PHF1 Human)

Tags

HaloTag_3XFLAG

Expression

Mammalian

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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