We narrowed to 443 results for: DHFR
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Plasmid#110827PurposeBacterial expression of E.coli DHFR D27S/Y100F mutantDepositorInsertecDHFR
ExpressionBacterialMutationD27 mutated to S27, Y100 mutated to F100PromoterT7Available SinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pELPS-eDHFR-YFP-T2A-Renilla
Plasmid#193253PurposeTriple reporter plasmid in pELPS with eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferaseDepositorInsertThe eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferase
UseLentiviralTagsRenilla luciferase (rLuc) and yellow fluorescent …MutationNonePromoterEF1aAvailable SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EGFP-eDHFR(69K6)-MCS
Plasmid#172100PurposeMammalian expression of a protein of interest fused to the C-terminus of EGFP-eDHFR(69K6)DepositorInsertEGFP-eDHFR(69K6)-20aa
ExpressionMammalianMutationeDHFR(69K6): Hexalysine (K6) sequence is inserted…PromoterCMVAvailable SinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-3XHA-eDHFR-NAT
Plasmid#188926PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-eDHFR-NAT
ExpressionYeastAvailable SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTetON-DHFRdd-SpvB; CMV-mCherry
Plasmid#89463PurposeTet/Dox-inducible expression of DHRFdd-SpvB (Salmonella SpvB 375-591) plus constitutive expression of mCherry in mammalian cells (Dual-promoter)DepositorInsertDeAct-SpvB (spvB Salmonella enterica)
TagsE. coli DHFRdd destabilization domain and mCherry…ExpressionMammalianMutationTruncation spanning SpvB amino acids 375-591 (mon…PromoterTRE3GAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ecDHFR(DD)-MS2CP-VPg(FCV)
Plasmid#167313PurposeTemplate pDNA to prepare DD-CaVT mRNADepositorInsertDD-CaVT
UseSynthetic BiologyTagsDYK-tagExpressionMammalianPromoterCMVAvailable SinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5 HA3-eDHFR-T2A-puro
Plasmid#86395Purposefor C-terminal tagging with eDHFR trimethoprim regulated degron with puro selectionDepositorInsertHA3 eDHFR T2A puro
UseCRISPRTagsHA3 eDHFR T2A puroMutationR12Y, G67S and Y100I mutations in E coli DHFRAvailable SinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCSIIpuro-mNG-eDHFR(69K6)-RasGEF
Plasmid#172104PurposeMammalian expression of RasGRF1(Cdc25) fused to mNeonGreen-eDHFR(69K6)DepositorInsertmNeonGreen-eDHFR(69K6)-RasGRF1(Cdc25) (RASGRF1 Human)
UseLentiviralExpressionMammalianPromoterEF1-alphaAvailable SinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MCS-eDHFR(69K6)-EGFP
Plasmid#172101PurposeMammalian expression of a protein of interest fused to the N-terminus of eDHFR(69K6)-EGFPDepositorInsert22aa-eDHFR(69K6)-EGFP
ExpressionMammalianMutationeDHFR(69K6): Hexalysine (K6) sequence is inserted…PromoterCMVAvailable SinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5 HA3-eDHFR-T2A-neo
Plasmid#86396Purposefor C-terminal tagging with eDHFR trimethoprim regulated degron with neo selectionDepositorInsertHA3 eDHFR T2A neo
UseCRISPRTagsHA3 eDHFR T2A neoMutationR12Y, G67S and Y100I mutations in E coli DHFRAvailable SinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only