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Plasmid#172101PurposeMammalian expression of a protein of interest fused to the N-terminus of eDHFR(69K6)-EGFPDepositorInsert22aa-eDHFR(69K6)-EGFP
UseTagsExpressionMammalianMutationeDHFR(69K6): Hexalysine (K6) sequence is inserted…PromoterCMVAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pELPS-eDHFR-YFP-T2A-Renilla
Plasmid#193253PurposeTriple reporter plasmid in pELPS with eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferaseDepositorInsertThe eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferase
UseLentiviralTagsRenilla luciferase (rLuc) and yellow fluorescent …ExpressionMutationNonePromoterEF1aAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5 HA3-eDHFR-T2A-puro
Plasmid#86395Purposefor C-terminal tagging with eDHFR trimethoprim regulated degron with puro selectionDepositorInsertHA3 eDHFR T2A puro
UseCRISPRTagsHA3 eDHFR T2A puroExpressionMutationR12Y, G67S and Y100I mutations in E coli DHFRPromoterAvailable sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Puro DHFR.dn-cHSF1
Plasmid#134729PurposeLentiviral expression vector for constitutively active dominant-negative HSF1 fused to DHFR destabilized domainDepositorInsertDHFR.dn-cHSF1 (HSF1 Human)
UseLentiviralTagsDHFRExpressionMammalianMutationDeletion of amino acids 186-202, 379−529PromoterCMVAvailable sinceAug. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLinker-AID-3xHA-DHFR-LoxP
Plasmid#86669Purposefor generation of AID strains using CRISPR tagging technologyDepositorInsertLinker-AID-3xHA-HXGPRT 3'UTR
UseExpression in toxoplasma gondiiTagsExpressionMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAc5 HA3-eDHFR-T2A-neo
Plasmid#86396Purposefor C-terminal tagging with eDHFR trimethoprim regulated degron with neo selectionDepositorInsertHA3 eDHFR T2A neo
UseCRISPRTagsHA3 eDHFR T2A neoExpressionMutationR12Y, G67S and Y100I mutations in E coli DHFRPromoterAvailable sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDN0606 (pDEST-DHFR F[3] N-term,HygR)
Plasmid#210488PurposepDEST vector to tag gene of interest with DHFR F[3] on the N-terminus to perform DHFR-PCA in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…TagsExpressionYeastMutationPromoterADH1Available sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKB12 (pDEST-DHFR F[3] C-term, HygR)
Plasmid#210486PurposepDEST vector to tag gene of interest with DHFR F[3] on the C-terminus to perform DHFR-PCA in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…TagsExpressionYeastMutationPromoterADH1Available sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-mNG-eDHFR(69K6)-G-alpha-q
Plasmid#172106PurposeMammalian expression of Gαq fused to mNeonGreen-eDHFR(69K6)DepositorInsertmNeonGreen-eDHFR(69K6)-Gαq (GNAQ Human)
UseTagsExpressionMammalianMutationGαq: C9S/C10S/Q209LPromoterCAGAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSL24a-Linker-smHA-FP-DHFR-LoxP
Plasmid#166238PurposeTagging of weakly expressed genes, floxed DHFR markerDepositorInsertSpaghetti monster HA
UseTagsExpressionMutationnonePromoterAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only