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Showing: 461 - 480 of 13545 results

  4. VP12

    Plasmid
    #72247
    Purpose
    Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A and Q926A
    Promoter
    CMV
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72247-plasmid-map-sequence-id-131866

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  13. mCherry-OptoPB

    Plasmid
    #113935
    Purpose
    Express a LOV2 fusion protein to enable light-dependent cytosol-to-PM translocation and dissect protein-phosphoinositide interactions
    Depositor
    Yubin Zhou
    Article
    He et al Chem Sci. 2017 Aug 1;8(8):5275-5281. doi
    Insert
    OptoPB
    Use
    Tags
    mCherry
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    113935-plasmid-map-sequence-id-367689

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  14. LiPOP1

    Plasmid
    #168264
    Purpose
    A single-component optogenetic tool for photoswitchable necroptosis
    Depositor
    Yubin Zhou
    Article
    He et al Adv Sci (Weinh). 2021 May 6;8(13):210042
    Insert
    MLKL(1-125) (MLKL Human)
    Use
    Tags
    mCherry, CRY2-PHR
    Expression
    Mammalian
    Mutation
    H15A, K16A, R17A
    Promoter
    CMV
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    168264-plasmid-map-sequence-id-334504

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  15. MSP2292

    Plasmid
    #70707
    Purpose
    Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #2
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    E782K, N968K and R1015H in SaCas9
    Promoter
    T7
    Available since
    Dec. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    70707-plasmid-map-sequence-id-127770

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  19. GFP-LiMETER-v2

    Plasmid
    #113934
    Purpose
    Also termed as OptoPBer; Express an STIM1-derived optical tether to label and interrogate ER-plasma membrane contact sites (MCS) with light; visualized by GFP
    Depositor
    Yubin Zhou
    Article
    He et al Chem Sci. 2017 Aug 1;8(8):5275-5281. doi
    Insert
    LOV2 and Stim1 polybasic domain (PB)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    113934-plasmid-map-sequence-id-225307

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Showing: 461 - 480 of 13545 results