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Showing: 691 - 720 of 2378 results

  1. pT2/shαSMA/GFP4_Seq2/clone1

    Plasmid
    #201402
    Purpose
    Knockdown of alpha-SMA. Construct has inverted repeats to be used in Sleeping Beauty transposon system.
    Depositor
    Maria Castro
    Insert
    alpha-SMA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    201402-plasmid-map-sequence-id-394260

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  2. SGEP-sh-Hs-PHGDH-1968

    Plasmid
    #188673
    Purpose
    shRNA
    Depositor
    Lewis Cantley
    Insert
    shPHGDH (PHGDH Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    188673-plasmid-map-sequence-id-375111

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  3. SGEP-sh-Hs-PHGDH-1967

    Plasmid
    #188672
    Purpose
    shRNA
    Depositor
    Lewis Cantley
    Insert
    shPHGDH (PHGDH Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    188672-plasmid-map-sequence-id-375054

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  4. pTSHZ1.1.0-gDNA

    Plasmid
    #132434
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    TSHZ1 (TSHZ1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    132434-plasmid-map-sequence-id-266216

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  6. pSB700-ACTC1-Puro

    Plasmid
    #128324
    Purpose
    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (human cells)
    Depositor
    Alejandro Chavez
    Insert
    gRNA against human ACTC1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    128324-plasmid-map-sequence-id-254244

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  7. mPLD3-sgRNA-Cas9-mcherry

    Plasmid
    #199700
    Purpose
    encodes sgRNA for mouse PLD3 KO, (target 217-223aa) plus Cas9-P2A-mCherry
    Depositor
    David Nemazee
    Insert
    hSpCas9
    Use
    CRISPR
    Tags
    P2A-RFP
    Expression
    Mammalian
    Mutation
    Promoter
    U6 promoter
    Available since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    199700-plasmid-map-sequence-id-390617

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  8. ipUSEPR-sg-Hs-PHGDH-int1

    Plasmid
    #188681
    Purpose
    control sgRNA
    Depositor
    Lewis Cantley
    Insert
    sgPHGDH intron control (PHGDH Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    188681-plasmid-map-sequence-id-375018

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  9. pICSL4723_ Rac5

    Plasmid
    #126885
    Purpose
    Genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 module, nptII resistance gene, and specific sgRNA modules on the binary plasmid pICSL4723.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    126885-plasmid-map-sequence-id-249710

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  10. pICSL4723_ Rac4

    Plasmid
    #126884
    Purpose
    Genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 module, nptII resistance gene, and specific sgRNA modules on the binary plasmid pICSL4723.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    126884-plasmid-map-sequence-id-249713

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  11. pENTRY4_ Rac4

    Plasmid
    #126896
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    126896-plasmid-map-sequence-id-250171

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  12. pENTRY4_Rac5

    Plasmid
    #126897
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    126897-plasmid-map-sequence-id-250096

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  13. bU6-sgMark2-mU6-sgMark3-hU6-sgMark4

    Plasmid
    #177235
    Purpose
    Expresses Mark2 (bU6), Mark3 (mU6) and Mark4 (hU6) gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgMark2/sgMark3/sgMark4
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    bU6/mU6/hU6
    Available since
    Jan. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    177235-plasmid-map-sequence-id-352696

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  14. bU6-sgNeo1-mU6-sgNuak1-hU6-sgNuak2

    Plasmid
    #177234
    Purpose
    Expresses Neomycin (bU6), Nuak1 (mU6) and Nuak2(hU6) gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgNeo1/sgNuak1/sgNuak2
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    bU6/mU6/hU6
    Available since
    Jan. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    177234-plasmid-map-sequence-id-352695

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  15. bU6-sgNeo1-mU6-sgAmpk1-hU6-sgAmpk2

    Plasmid
    #177233
    Purpose
    Expresses Neomycin (bU6), Ampk1 (mU6) and Ampk2(hU6) gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgNeo1/sgPrkaa1/sgPrkaa2
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    bU6/mU6/hU6
    Available since
    Jan. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    177233-plasmid-map-sequence-id-352662

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  16. pNSU299_CPR1_1

    Plasmid
    #166074
    Purpose
    Plasmid for constituive spCas9 and tet-inducible CPR1 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    CPR1 (CPR1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166074-plasmid-map-sequence-id-330357

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  17. pNSU299_LHS1

    Plasmid
    #166092
    Purpose
    Plasmid for constituive spCas9 and tet-inducible LHS1 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    LHS1 (LHS1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    166092-plasmid-map-sequence-id-329611

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  18. pDual_dsCas9_Blast_hs_TP73

    Plasmid
    #214691
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two sgRNA sequences against human TP73
    Depositor
    Kevin Janes
    Insert
    dgRNA_TP73 (TP73 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214691-plasmid-map-sequence-id-428772

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  19. Lentiviral sgRNA human CD19

    Plasmid
    #155289
    Purpose
    Lentiviral expression of sgRNA against human CD19
    Depositor
    Martin Carroll
    Insert
    CD19 (CD19 Human)
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    155289-plasmid-map-sequence-id-309995

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  20. pDual_dsCas9_Venus_hs_TP73

    Plasmid
    #214685
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human TP73
    Depositor
    Kevin Janes
    Insert
    dgRNA_TP73 (TP73 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214685-plasmid-map-sequence-id-428818

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  21. pDual_dsCas9_BFP_hs_TP73

    Plasmid
    #214687
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-BFP with two sgRNA sequences against human TP73
    Depositor
    Kevin Janes
    Insert
    dgRNA_TP73 (TP73 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214687-plasmid-map-sequence-id-428806

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  22. 1167A

    Plasmid
    #218233
    Purpose
    HDR plasmid for inserting Ceratitis capitata transformer female intron into the white pupae gene
    Depositor
    Omar Akbari
    Insert
    putative metabolite transport protein
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    May 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    218233-plasmid-map-sequence-id-433264

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  23. ipUSEPR-sg-Hs-ACE2-A2

    Plasmid
    #188687
    Purpose
    sgRNA
    Depositor
    Lewis Cantley
    Insert
    sgACE2 (ACE2 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    188687-plasmid-map-sequence-id-375123

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  24. pNSU299_RPL22A

    Plasmid
    #166079
    Purpose
    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of RPL22A for double stranded break formation in yeast.
    Depositor
    Rebecca Burgess
    Insert
    Promoter of RPL22A (RPL22A Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    166079-plasmid-map-sequence-id-329605

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  25. LentiCRISPR_Hs_MBD3_ex2

    Plasmid
    #62623
    Purpose
    Encodes gRNA which targets human MBD3
    Depositor
    Paul Wade
    Insert
    MBD3 gRNA (MBD3 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    EFS
    Available since
    Nov. 30, 2018
    Availability
    Academic Institutions and Nonprofits only

  26. pTRIPZ (M)-HT-Cbx2

    Plasmid
    #82510
    Purpose
    Expresses Halotag-Cbx2 fusion proteins in mammalian cells
    Depositor
    Xiaojun Ren
    Insert
    Chromobox Homolog 2 (CBX2 Human)
    Use
    Lentiviral
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    aggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaacc…
    Available since
    Dec. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    82510-plasmid-map-sequence-id-156621

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  27. pAIO-Ef1a-PE2-GFP:KCNQ2-C201R

    Plasmid
    #185060
    Purpose
    Ef1a driven PE2 plasmid with pegRNA for editing C201R mutation in KCNQ2 gene. See Addgene plasmid #184445
    Depositor
    Bobby Koeleman
    Insert
    U6:pegRNA:scaffold:PBS+RT template
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Nov. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    185060-plasmid-map-sequence-id-375730

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  28. TMPRSS2 g1 + Cas9

    Plasmid
    #153015
    Purpose
    A guide RNA targeting TMPRSS2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2
    Depositor
    Jason Sheltzer
    Insert
    Cas9 + TMPRSS2 gRNA
    Use
    CRISPR and Lentiviral
    Tags
    TagBFP2
    Expression
    Mutation
    Promoter
    Available since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    153015-plasmid-map-sequence-id-298676

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  29. pKLV-gRNA-CTBP2-MGMT_1

    Plasmid
    #136419
    Purpose
    Lentiviral expression of gRNAs targeting intron 1 of human CTBP2 and intron 1 of human MGMT. Also constitutively expresses Puromycin fused to TagBFP.
    Depositor
    Massimo Squatrito
    Insert
    U6_sgRNA(CTBP2)_U6_sgRNA(MGMT)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    136419-plasmid-map-sequence-id-271761

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  30. pCFD3-dUG-DH1

    Plasmid
    #138963
    Purpose
    Used to edit the endogenous Drosophila kkv gene to encode a fluorescent Chitin Synthase.
    Depositor
    Paul Adler
    Article
    Adler et al bioRxiv 2020
    Insert
    DH1 oligo
    Use
    CRISPR
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    138963-plasmid-map-sequence-id-274780

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Showing: 691 - 720 of 2378 results