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Plasmid#228200PurposeAAV vector expressing GFP and HaloTag-HAPLN1 under constitutive promoterDepositorInsertHAPLN1 (Hapln1 Mouse)
UseAAVTagsExpressionMammalianMutationPromoterconstitutive CAG promoterAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV pSYN eGFP T2A SP HALO HAPLN1
Plasmid#228199PurposeAAV vector expressing GFP and HaloTag-HAPLN1 under synaptophysin promoterDepositorInsertHAPLN1 (Hapln1 Mouse)
UseAAVTagsExpressionMammalianMutationPromoterhuman synaptophysin promoterAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHR-eGFP-CD86tm-HALOtag-FKBP(f36v)
Plasmid#200281PurposeMembrane protein fusion with extracellular GFP and intracellular FKBP(f36v) dimerization domain and HALOtagDepositorInserteGFP-CD86tm-HaloTag-FKBP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE pGK LysoFlux (HaloTag7-mGFP-Galectin3)
Plasmid#215009PurposeExpress a Lysophagy reporter in mammalian cells (untested)DepositorInsertLGALS3 (LGALS3 Human)
UseLentiviralTagsHaloTag(7)-mGFPExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
T7-LacO-Halo-RGD-STReTCh (Force Inert)
Plasmid#216710PurposeExpresses HaloTag-RGD-STReTCh Force Inert Control in bacteriaDepositorInsertHaloTag-RGD-STReTCh
UseTags6xHis and HaloTagExpressionBacterialMutationPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-tdMCP2-GyG-(GGS)X6-HaloTag C261
Plasmid#171434PurposeExpression of tdMCP2-GyG-(GGS)X6-HaloTag C261 in mammalian cellsDepositorInserttdMCP2-GyG-(GGS)X6-HaloTag C261
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR4 CDS A. thaliana IMPORTIN ALPHA 3 / MOS6
Plasmid#175820PurposepENTR plasmid with CDS of Arabidopsis thaliana IMPORTIN ALPHA ISOFORM 3 / MOS6, lacks start ATG and includes stop codon for N-terminal epitope tagsDepositorInsertIMPORTIN ALPHA ISOFORM 3 (MOS6 Mustard Weed)
UsePentr plasmid for gateway cloningTagsnoneExpressionMutationPromoternoneAvailable sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR/D-Topo genomic A. thaliana IMPORTIN ALPHA 4
Plasmid#175815PurposepENTR plasmid with genomic sequence of Arabidopsis thaliana IMPORTIN ALPHA ISOFORM 4 (AT1G09270) without stop codon for C-terminal epitope tagsDepositorInsertIMPORTIN ALPHA ISOFORM 4 (IMPA-4 Mustard Weed)
UsePentr plasmid for gateway cloningTagsnoneExpressionMutationPromoternoneAvailable sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR/D-Topo CDS A. thaliana IMPORTIN ALPHA 2
Plasmid#175816PurposepENTR plasmid with CDS of Arabidopsis thaliana IMPORTIN ALPHA ISOFORM 2 (AT4G16143.1) without stop codon for C-terminal epitope tagsDepositorInsertIMPORTIN ALPHA ISOFORM 2 (IMPA-2 Mustard Weed)
UsePentr plasmid for gateway cloningTagsnoneExpressionMutationnatural polymorphism P65S, annotated in Genbank f…PromoternoneAvailable sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR/D-Topo CDS A. thaliana IMPORTIN ALPHA 9
Plasmid#175817PurposepENTR plasmid with CDS of Arabidopsis thaliana IMPORTIN ALPHA ISOFORM 9 (AT5G03070.1) without stop codon for C-terminal epitope tagsDepositorInsertIMPORTIN ALPHA ISOFORM 9 (IMPA-9 Mustard Weed)
UsePentr plasmid for gateway cloningTagsnoneExpressionMutationPromoternoneAvailable sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4 3xFLAG Halo R119A K120A nsp1
Plasmid#175428PurposeExpresses R119A K120A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.DepositorInsertnsp1 R119A K120A
UseTags3XFLAG HaloExpressionMammalianMutationN-terminal mutants R119A K120APromoterCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4 3xFLAG Halo E36A E37A nsp1
Plasmid#175429PurposeExpresses E36A E37A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.DepositorInsertnsp1 E36A E37A
UseTags3XFLAG HaloExpressionMammalianMutationN-terminal mutants E36A E37APromoterCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2 Halo-Tev- HSPB7 (zebrafish) C117S
Plasmid#108915PurposeExpress C117S mutant of Zebrafish Halo-Tev-HSPB7 in mammalian cellsDepositorInsertHalo-TEV-HSPB7
UseTagsHalo-TEVExpressionMammalianMutationCysteine 117 to SerinePromoterSP6Available sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2 Halo-Tev- HSPB7(zebrafish) C49S
Plasmid#108914PurposeExpress C49S mutant of Zebrafish Halo-Tev-HSPB7 in mammalian cellsDepositorInsertHalo-TEV-HSPB7
UseTagsHalo-TEVExpressionMammalianMutationCysteine 49 to SerinePromoterSP6Available sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn SYT7alpha-HaloTag TMD Cys-Ala
Plasmid#179716PurposeLentiviral expression of mouse Syt7 palmitoylation site mutant with C terminal HaloTagDepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pLX304 CMV HA-HaloTag-FRB-sTurboID-high affinity (C)
Plasmid#153005PurposeExpresses high-affinity split-TurboID C-terminal fragment in mammalian cellsDepositorInsertsTurboID-high affinity (C) (G79)
UseLentiviralTagsFRB, HA, and HaloTagExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE pGK LC3Flux (HaloTag7-mGFP-LC3-STOP)
Plasmid#215015PurposeExpress an autophagy (LC3) reporter in mammalian cells (untested)DepositorInsertMAP1LC3B (Map1lc3b Rat)
UseLentiviralTagsHaloTag(7)-mGFPExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-Ef1a-PP7 coat protein-Halo-GB1x3-WPRE
Plasmid#198337PurposePB plasmid expressing Halo-tagged PP7 coat proteinDepositorInsertPP7 coat protein 3x Halo-GB1
UsePiggybacTagsHaloTagExpressionMammalianMutationPromoterEf1aAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti UBC MitoFlux (pSu9-HaloTag7-mGFP-STOP)
Plasmid#215010PurposeExpress a Mitophagy reporter in mammalian cells (untested)DepositorInsertpSu9 (Presequence of Fo-ATPase subunit 9)
UseLentiviralTagsHaloTag(7)-mGFPExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GluA1-scFv-Halo-HRV-BiotinAP-HIS
Plasmid#230058PurposeExpresses a single chain variable fragment (scFv) for anti-GluA1 fused to Halotag with an HRV (PreScission) protease cleavage site, biotin acceptor peptide and 6XHIS purification tag.DepositorInsertAnti-GluA1 single chain variable fragment (scFv)
UseTags6X HIS, HRV 3C PreScission protease, HaloTag, and…ExpressionMammalianMutationPromoterChicken beta actin (CAG)Available sinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GluA2-scFv-Halo-HRV-BiotinAP-HIS
Plasmid#230059PurposeExpresses a single chain variable fragment (scFv) for anti-GluA2 fused to Halotag with an HRV (PreScission) protease cleavage site, biotin acceptor peptide and 6XHIS purification tag.DepositorInsertAnti-GluA2 single chain variable fragment (scFv)
UseTags6X HIS, HRV 3C PreScission protease, HaloTag, and…ExpressionMammalianMutationPromoterChicken beta actin (CAG)Available sinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHR-scFv-GCN4-HaloTag-GB1-NLS-dWPRE
Plasmid#106303PurposeThe plasmid encodes the scFv against GCN4 peptide fused with HaloTag and a NLS for SunTag imagingDepositorInsertscFv-GCN4-HaloTag-GB1-NLS
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pME Actin Vhh Halo Tag (JDW 1223)
Plasmid#224498PurposeGateway compatible middle entry clone containing an Actin nanonbody fused to a flexible linker and Halo Tag (For visualizing actin, actin chromobody)DepositorInsertActin-Vhh-Halo-Tag
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_Beta4Gal-HaloTag7-T2A-Lyn11-SNAPf
Plasmid#175524PurposeCo-expression of HaloTag7 (gogli) and SNAPf-tag (membrane inner leaflet) in mammalian cellsDepositorInsertBeta4Gal-HaloTag7 and Lyn11-SNAPf
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 ERFlux (Bip-HaloTag7-mGFP-KDEL-STOP)
Plasmid#215002PurposeShuttling vector for an ERphagy reporterDepositorInsertKDEL
UseGatewayTagsHaloTag(7)-mGFPExpressionMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti UBC LC3Flux (HaloTag7-mGFP-LC3-STOP)
Plasmid#215014PurposeExpress an autophagy (LC3) reporter in mammalian cells (untested)DepositorInsertMAP1LC3B (Map1lc3b Rat)
UseLentiviralTagsHaloTag(7)-mGFPExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMIR-DsRed-IRES-His-Halo-Tev-Keap1
Plasmid#58240Purposeexpresses halo tagged human Keap1 proteinDepositorInsertKelch like ECH associated protein 1 (KEAP1 Human)
UseTagsHis-Halo-TevExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4 3xFLAG Halo delta 122-130 nsp1
Plasmid#175425PurposeExpresses delta 122-130 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.DepositorInsertCoV2 delta 122-130 nsp1
UseTags3XFLAG HaloExpressionMammalianMutationPromoterCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4 3xFLAG Halo delta 118-180 nsp1
Plasmid#175424PurposeExpresses delta 118-180 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.DepositorInsertCoV2 delta 118-180 nsp1
UseTags3XFLAG HaloExpressionMammalianMutationN-terminus of nsp1PromoterCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2 Halo-Tev- HSPB7 (zebrafish) C49S and C117S
Plasmid#108913PurposeExpress C49S/C117S double mutant of Zebrafish Halo-Tev-HSPB7 in mammalian cellsDepositorInsertHalo-TEV-HSPB7
UseTagsHalo-TEVExpressionMammalianMutationCysteine 49 to Serine, Cysteine 117 to SerinePromoterSP6Available sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
TL1 alphaDdCBE A1 (DddA6-C) - TL1 alphaL_DdCBE 2 (left, DddA11-C)
Plasmid#221994PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE A1 repeats-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationTALE: Q231R, W232G, S233A | DddA: T1413IPromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
T7-LacO-Halo-RGD-PILATeS (200nM, Force Inert)
Plasmid#216717PurposeExpresses HaloTag-RGD-PILATeS Force Inert Control with 200 nM BiT tag in bacteriaDepositorInsertHaloTag-RGD-PILATeS
UseTags6xHis and HaloTagExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
T7-LacO-Halo-RGD-PILATeS (700pM, Force Inert)
Plasmid#216715PurposeExpresses HaloTag-RGD-PILATeS Force Inert Control with 700 pM BiT tag in bacteriaDepositorInsertHaloTag-RGD-PILATeS
UseTags6xHis and HaloTagExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE pGK MitoFlux (pSu9-HaloTag7-mGFP-STOP)
Plasmid#215011PurposeExpress a Mitophagy reporter in mammalian cells (untested)DepositorInsertpSu9 (Presequence of Fo-ATPase subunit 9)
UseLentiviralTagsHaloTag(7)-mGFPExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4 3xFLAG Halo E55A E57A K58A nsp1
Plasmid#175427PurposeExpresses E55A E57A K58A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.DepositorInsertnsp1 E55A E57A K58A
UseTags3XFLAG HaloExpressionMammalianMutationN-terminal mutants E55A E57A K58APromoterCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT_HaloTag7-SNAP-tag2-NLS-P2A-NLS-mTurquoise2
Plasmid#226514PurposeMamalian cell expression of nuclear HaloTag7-SNAP-tag2 and nuclear mTurquoise2DepositorInsertHaloTag7-SNAP-tag2-NLS-P2A-NLS-mTurquoise2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
TL1 alphaL_DdCBE 3-4 (left, DddA11-C) - TL1 alphaDdCBE 1 (left, DddA11-C)
Plasmid#221998PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alphaL-3/4 left, alpha-1 left)-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationTALE: Q231R, W232G, S233A | DddA: T1413IPromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO_CalR-HaloTag7-P30-SNAP-tag-KDEL
Plasmid#182012PurposeCMV overexpression of HaloTag7 and SNAP-tag fusion in the endoplasmic reticulum of mammalian cell linesDepositorInsertCalR-HaloTag7-P30-SNAP-tag-KDEL
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCA26-pLenti.CAG.-NLS-dCas13b-NLS-VPR-T2A-HaloTag
Plasmid#199457PurposeExpression of dCas13-VPR in mammalian cellsDepositorInsertdCas13
UseLentiviralTagsVPR-T2A-HaloTagExpressionMammalianMutationNuclease-dead H133A and H1058APromoterCAGAvailable sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Dsred-IRES-His-Halo-Tev-Keap C151S
Plasmid#62458Purposeexpresses his-tagged C151S human Keap1 proteinDepositorInsertKeap1 (KEAP1 Human)
UseTagsHis-Halo-TevExpressionMammalianMutationChanged Cysteine 151 of Keap1 to serinePromoterCMVAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only