We narrowed to 1,873 results for: FRT

1. pcDNA5-FRT-TO-Ubc13

   Plasmid

   #212806

   Purpose

   Constitutive or doxycycline-inducible expression of budding yeast Ubc13 in mammalian cells

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   Ubc13
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA5-FRT-TO-Mms2

   Plasmid

   #212807

   Purpose

   Constitutive or doxycycline-inducible expression of budding yeast Mms2 in mammalian cells

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   Mms2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA5-FRT-TO-EGFP-N

   Plasmid

   #210270

   Purpose

   N-terminal EGFP-tagging of inserts for inducible expression. Use with Flp-In T-Rex system.

   Depositor

   Niels Mailand

   Article

   Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

   Type

   Empty backbone

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCDNA5/FRT-ChemoB

   Plasmid

   #193808

   Purpose

   CMV overexpression of ChemoB in mammalian cell lines

   Depositor

   Julien Hiblot

   Article

   Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

   Insert

   ChemoB
   

   Use

   Tags

   10xHis

   Expression

   Mammalian

   Mutation

   EBFP2: N39Y, V206K, T225R; HT7: E143R, E147R, L27…

   Promoter

   CMV

   Available Since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCDNA5/FRT-ChemoY

   Plasmid

   #193810

   Purpose

   CMV overexpression of ChemoY in mammalian cell lines

   Depositor

   Julien Hiblot

   Article

   Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

   Insert

   ChemoY
   

   Use

   Tags

   10xHis

   Expression

   Mammalian

   Mutation

   Venus:A206K, T225R; HT7: E143R, E147R, L271E

   Promoter

   CMV

   Available Since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCDNA5/FRT-ChemoR

   Plasmid

   #193811

   Purpose

   CMV overexpression of ChemoR in mammalian cell lines

   Depositor

   Julien Hiblot

   Article

   Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

   Insert

   ChemoR
   

   Use

   Tags

   10xHis

   Expression

   Mammalian

   Mutation

   mScarlet: D201K

   Promoter

   CMV

   Available Since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA5-FRT-TO-nsp7

   Plasmid

   #201025

   Purpose

   Expresses SARS-CoV-2 nsp7 under control of a tetracycline-inducible promoter in mammalian.

   Depositor

   Sharon Rozovsky

   Article

   Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

   Insert

   non-structural protein 7 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-tet-ON

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LLP728_L2_pV01_0I_FRT-TB-rLUC_TCTP-fLUC

   Plasmid

   #192371

   Purpose

   To test for the repressive potential of the Transcription Block (TB) with the FRT sites around it.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-TB-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LLP732_L2_pV01_0I_FRT-35Ster-Rluc_lacZ

   Plasmid

   #192375

   Purpose

   To test for the repressive potential of the 35S terminator from CaMV with the FRT sites around it.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-35Ster-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LLP731_L2_pV01_0I_FRT-OCStrunc-Rluc_lacZ

    Plasmid

    #192374

    Purpose

    To test for the repressive potential of the OCS variant (truncation) with the FRT sites around it.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCStrunc-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LLP742_L2pV1_CR_FRT-Rluc_TCTP-B3o_lacZ

    Plasmid

    #192386

    Purpose

    To test for if B3 is able to de-repress a circuit with FRT, to rule out cross-reactivity between these recombinases.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCS-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA5/FRT/TO_STf

    Plasmid

    #182000

    Purpose

    CMV overexpression of SNAP-tag fast in mammalian cell lines

    Depositor

    Kai Johnsson

    Article

    Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

    Insert

    STf
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Fast mutation on SNAP-tag E30R

    Promoter

    CMV

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA5/FRT/TO_CalR_STf_KDEL

    Plasmid

    #182004

    Purpose

    CMV overexpression of SNAP-tag fast in the endoplasmic reticulum of mammalian cell lines

    Depositor

    Kai Johnsson

    Article

    Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

    Insert

    CalR_STf_KDEL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Fast mutation on SNAP-tag E30R

    Promoter

    CMV

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA5/FRT/TO_STf_SKL

    Plasmid

    #182007

    Purpose

    CMV overexpression of SNAP-tag fast in the peroxisome of mammalian cell lines

    Depositor

    Kai Johnsson

    Article

    Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

    Insert

    STf_SKL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Fast mutation on SNAP-tag E30R

    Promoter

    CMV

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA5/FRT/TO/MACROD1

    Plasmid

    #172578

    Purpose

    For generation of FlpIn lines with untagged MACROD1

    Depositor

    Karla Feijs

    Article

    Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

    Insert

    MACROD1 (MACROD1 Human)
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA5/FRT/TO/MACROD2

    Plasmid

    #172579

    Purpose

    For generation of FlpIn lines with untagged MACROD2

    Depositor

    Karla Feijs

    Article

    Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

    Insert

    MACROD2 (MACROD2 Human)
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA5/FRT/TO/TARG1

    Plasmid

    #172580

    Purpose

    For generation of FlpIn lines with untagged TARG1

    Depositor

    Karla Feijs

    Article

    Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

    Insert

    TARG1 (OARD1 Human)
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA-FRT-TO-Nprot_GC3opt

    Plasmid

    #157730

    Purpose

    mammalian expression of untagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nprot_GC3opt (N SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Promoter

    Available Since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pcDNA5-FRT-TO-Orf6

    Plasmid

    #157726

    Purpose

    mammalian expression of untagged SARS-CoV-2 Orf6 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Orf6 (ORF6 SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available Since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pcDNA5-FRT-TO-Nsp15

    Plasmid

    #157720

    Purpose

    mammalian expression of untagged SARS-CoV-2 Nsp15 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp15 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available Since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge