We narrowed to 32,680 results for: IND

1. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA2b_(CJT93)

   Plasmid

   #226992

   Purpose

   pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 2b must be used with gRNA 1a or 1b

   Depositor

   Benjamin Kleinstiver

   Article

   Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

   Insert

   SpCas9 gRNA 2b to create OPA1-del_exon5
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA1b_(CJT92)

   Plasmid

   #226990

   Purpose

   pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 1b must be used with gRNA 2a or 2b

   Depositor

   Benjamin Kleinstiver

   Article

   Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

   Insert

   SpCas9 gRNA 1b to create OPA1-del_exon5
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUC19-U6-SpCas9_gRNA-OPA1-createR194G-A9-gRNA_(CJT87)

   Plasmid

   #226988

   Purpose

   pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-R194G cell line via adenine base editing (target base in position A9)

   Depositor

   Benjamin Kleinstiver

   Article

   Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

   Insert

   SpCas9 gRNA A9 to create OPA1-R194G
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSUC01

   Plasmid

   #177204

   Purpose

   chromogenic reporter for gene knockout in Streptomyces

   Depositor

   Wenjun Guan

   Article

   Dong et al J Microbiol Biotechnol. 2021 Dec 28;31(1

   Insert

   SaindC
   

   Use

   Synthetic Biology

   Tags

   NONE

   Expression

   Bacterial

   Mutation

   NONE

   Promoter

   TGTTCACATTCGAACGGTCTCTGCTTTGACAACATGCTGTGCGGTGTTG…

   Available since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pERB130

   Plasmid

   #58284

   Purpose

   const. Mis12-GFP-FKBP3; ind. FRB-mCH-Mad1

   Depositor

   Michael Lampson

   Article

   Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

   Inserts

   Mis12-GFP-FKBPx3
   inducible FRB-mCh-Mad1
   FKBP-targeted miRNA
   

   Use

   Tags

   GFP and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pERB127

   Plasmid

   #58283

   Purpose

   const. Mis12-GFP-FKBP3; ind. FRB-mCH(FseI)

   Depositor

   Michael Lampson

   Article

   Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

   Inserts

   Mis12-GFP-FKBPx3
   inducible FRB-mCh
   FKBP-targeted miRNA
   

   Use

   Tags

   GFP and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pERB160

   Plasmid

   #58285

   Purpose

   const. Mis12-FKBP3; ind. FRB-mCH-Mad1

   Depositor

   Michael Lampson

   Article

   Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

   Inserts

   Mis12-FKBPx3
   inducible FRB-mCh-Mad1
   FKBP-targeted miRNA
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pERB122

   Plasmid

   #58282

   Purpose

   const: miRFKBP5_GFP_IRES_Puro; ind: mC-FRB

   Depositor

   Michael Lampson

   Article

   Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

   Inserts

   FKBP-targeted miRNA
   GFP
   Puromycin resistance
   inducible mCherry-FRB
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pERB171

   Plasmid

   #58289

   Purpose

   const. Mis12-FKBP3; ind. FRB-mCH-Mad1^485-718

   Depositor

   Michael Lampson

   Article

   Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

   Inserts

   Mis12-FKBPx3
   inducible FRB-mCh-Mad1^485-718
   FKBP-targeted miRNA
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pERB170

    Plasmid

    #58288

    Purpose

    const. Mis12-FKBP3; ind. FRB-mCH-Mad1^485-584

    Depositor

    Michael Lampson

    Article

    Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

    Inserts

    Mis12-FKBPx3
    inducible FRB-mCh-Mad1^485-584
    FKBP-targeted miRNA
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pERB174

    Plasmid

    #58291

    Purpose

    const. Mis12-GFP-FKBP3; ind. FRB-mCH-Mad1^549-718

    Depositor

    Michael Lampson

    Article

    Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

    Inserts

    Mis12-GFP-FKBPx3
    inducible FRB-mCh
    FKBP-targeted miRNA
    FRB-mCh-Mad1^549-718
    

    Use

    Tags

    GFP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pERB172

    Plasmid

    #58290

    Purpose

    const. Mis12-GFP-FKBP3; ind. FRB-mCH-Mad1^485-718-AAA

    Depositor

    Michael Lampson

    Article

    Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

    Inserts

    Mis12-GFP-FKBPx3
    inducible FRB-mCh
    FKBP-targeted miRNA
    FRB-mCh-Mad1^485-718-AAA
    

    Use

    Tags

    GFP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pERB161

    Plasmid

    #58286

    Purpose

    const. Mis12-GFP-FKBP3; ind. FRB-mCH-Mad1^485-584 (MID)

    Depositor

    Michael Lampson

    Article

    Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

    Inserts

    Mis12-GFP-FKBPx3
    inducible FRB-mCh-Mad1^485-584
    FKBP-targeted miRNA
    

    Use

    Tags

    GFP and mCherry

    Expression

    Mammalian

    Mutation

    amino acids 485-584 of Mad1

    Promoter

    Available since

    Feb. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDL52 (clone45)

    Plasmid

    #188578

    Purpose

    High-yield production of coenzyme F420 in E. coli

    Depositor

    Gerald Lackner

    Article

    Last et al Metab Eng. 2022 Jul 18. pii: S1096-7176(

    Inserts

    ribA
    ribD
    yigB
    fbiC
    cofC
    cofD
    cofI
    cofE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimization, synthetic ribosome binding si…

    Promoter

    T7 variants

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDL52 (clone76)

    Plasmid

    #188579

    Purpose

    High-yield production of coenzyme F420 in E. coli

    Depositor

    Gerald Lackner

    Article

    Last et al Metab Eng. 2022 Jul 18. pii: S1096-7176(

    Inserts

    ribA
    ribD
    yigB
    fbiC
    cofC
    cofD
    cofI
    cofE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimization, synthetic ribosome binding si…

    Promoter

    T7 variants

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNAintron-CHIKV-Envelope protein

    Plasmid

    #215700

    Purpose

    Produces the Chikungunya virus envelope protein E3-E2-6K-E1

    Depositor

    Melinda Brindley

    Article

    Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

    Insert

    CHIKV E3-E2-6K-E1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pcDNAintron-CHIKV-capsid protein

    Plasmid

    #215701

    Purpose

    Produces the Chikungunya virus capsid protein

    Depositor

    Melinda Brindley

    Article

    Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

    Insert

    CHIKV capsid
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pcDNAintron-CDC50A-c3xFLAG

    Plasmid

    #215702

    Purpose

    Produces human CDC50A-c3xFLAG, a chaperone to phosphatidylserine flippase complexes

    Depositor

    Melinda Brindley

    Article

    Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

    Insert

    CDC50A (TMEM30A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. BtuF

    Plasmid

    #92209

    Purpose

    Expression of BtuF which is the vitamin B12 binding protein for BtuCD ABC Transporter

    Depositor

    Douglas Rees

    Article

    Borths et al Proc Natl Acad Sci U S A. 2002 Dec 24;99

    Insert

    BtuF (btuF )
    

    Use

    Tags

    6x His Tag and PelB Signal Sequence

    Expression

    Bacterial

    Mutation

    BtuF signal sequence removed and uses the PelB si…

    Promoter

    T7 Promoter

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNAintron-CHIKV-nsp3-c3xFLAG

    Plasmid

    #215696

    Purpose

    Produces the Chikungunya virus nonstructural protein 3 with a c-terminal 3xFLAG tag

    Depositor

    Melinda Brindley

    Article

    Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

    Insert

    CHIKV nsp3
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge