We narrowed to 2,266 results for: Kap

1. pJK558

   Plasmid

   #71704

   Purpose

   Produces Acetobacter aceti 1023 thioredoxin reductase 1 with Cys138>Ser mutant (AaTrxB1-C138S)

   Depositor

   T. J. Kappock

   Insert

   thioredoxin reductase 1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes cysteine-138 to serine

   Promoter

   T7

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJK505

   Plasmid

   #71594

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe mutant (AaPurE1fww)

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes tryptophan-34 to phenylalanine, additiona…

   Promoter

   T7

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJK506

   Plasmid

   #71596

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp165>Phe mutant (AaPurE1wwf)

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes tryptophan-165 to phenylalanine, addition…

   Promoter

   T7

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDC10

   Plasmid

   #75077

   Purpose

   Produces Escherichia coli glycinamide ribonucleotide synthetase (H6EcPurD)

   Depositor

   JoAnne Stubbe, T. J. Kappock

   Insert

   phosphoribosylglycinamide synthetase (purD Escherichia coli)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   adds unique SacII site at Thr95-Ala96 by a silent…

   Promoter

   T7

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRDB_345

   Plasmid

   #231113

   Purpose

   ABE activity-based selection cloning vector

   Depositor

   David Root, John Doench

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRDB_347

   Plasmid

   #231114

   Purpose

   CBE activity-based selection cloning vector

   Depositor

   David Root, John Doench

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJK131

   Plasmid

   #71548

   Purpose

   Produces Geobacillus stearothermophilus (Bacillus stearothermophilus) alanine racemase (alr)

   Depositor

   T. J. Kappock

   Article

   Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

   Insert

   Alanine racemase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGWF1

   Plasmid

   #18856

   Depositor

   Wolf Frommer

   Article

   Kaper et al Biotechnol Biofuels. 2008 Jun 3. 1(1):11

   Type

   Empty backbone

   Use

   Tags

   CFP and Venus

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 21, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pB80-empty

   Plasmid

   #174621

   Purpose

   Empty backbone

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJK611

    Plasmid

    #72532

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit (AaPurQ); large subunit (AaPurL)

    Depositor

    T. J. Kappock

    Inserts

    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 and n/a

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJK626

    Plasmid

    #72461

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Cys/Tyr154>Phe mutant (AaPurE1-S57C,Y154F)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Mutation changes serine-57 to cysteine, tyrosine-…

    Promoter

    T7

    Available since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJK691

    Plasmid

    #72381

    Purpose

    Produces Escherichia coli BolA, N-terminal His6 tag (H6EcBolA)

    Depositor

    T. J. Kappock

    Insert

    stationary-phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC (bolA Escherichia coli str. K-12 substr. MG1655)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJK292

    Plasmid

    #73371

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/His89>Asn mutant (AaPurE1-H59N,H89N)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changed histidine-59 to asparagine and histidine-…

    Promoter

    T7

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJK293

    Plasmid

    #73372

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Phe/His89>Phe mutant (AaPurE1-H59F,H89F)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changed histidine-59 to phenylalanine and histidi…

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJK294

    Plasmid

    #73373

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asp/His89>Asp mutant (AaPurE1-H59D,H89D)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changed histidine-59 to aspartate and histidine-8…

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJK528

    Plasmid

    #71625

    Purpose

    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Met105>Ser/Ile109>Trp mutant (TdPurE2-M105S,I109W)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylaminoimidazole carboxylase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes methionine-105 to serine, isoleucine-109 …

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJK439

    Plasmid

    #72478

    Purpose

    Produces Thermoplasma acidophilum citrate synthase pseudo-dimer

    Depositor

    T. J. Kappock

    Insert

    citrate synthase (artificial dimer)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    inserted NSTCLVPRGSPV sequence between C- and N-t…

    Promoter

    T7

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJK404

    Plasmid

    #72417

    Purpose

    Produces Acetobacter aceti 1023 Est12, N-terminal His6 tag

    Depositor

    T. J. Kappock

    Insert

    acyl-CoA thioesterase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    additional N-terminal 15 residues MSETYGTGQKEQHC,…

    Promoter

    T7

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJK268

    Plasmid

    #72436

    Purpose

    Produces Acetobacter aceti 1023 del7-GloA

    Depositor

    T. J. Kappock

    Insert

    lactoylglutathione lyase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Missing residues 1-7

    Promoter

    T7

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJK627

    Plasmid

    #72462

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Asp/Tyr154>Phe mutant (AaPurE1-S57D,Y154F)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Mutation changes serine-57 to aspartate, tyrosine…

    Promoter

    T7

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge