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Plasmid#124394PurposeE.Coli produced TET1-SID mutants (T898E) purified by FPLC nickel columnDepositorInsertGB1-His-TET1-878-911-T898E (TET1 Human)
UseTagsGB1-HistidineExpressionBacterialMutationT898EPromoterAvailable sinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-TET1-878-911-T898A(pL91)
Plasmid#124393PurposeE.Coli produced TET1-SID mutants (T898A) purified by FPLC nickel columnDepositorInsertGB1-His-TET1-878-911-T898A (TET1 Human)
UseTagsGB1-HistidineExpressionBacterialMutationT898APromoterAvailable sinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-TET1-878-911-L897A(pL91)
Plasmid#124392PurposeE.Coli produced TET1-SID mutants (L897A) purified by FPLC nickel columnDepositorInsertGB1-His-TET1-878-911-L897A (TET1 Human)
UseTagsGB1-HistidineExpressionBacterialMutationL897APromoterAvailable sinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-TET1-878-911-I894A(pL91)
Plasmid#124391PurposeE.Coli produced TET1-SID mutants (I894A) purified by FPLC nickel columnDepositorInsertGB1-His-TET1-878-911-I894A (TET1 Human)
UseTagsGB1-HistidineExpressionBacterialMutationI894APromoterAvailable sinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
SLK gRNA (BRDN0001147580)
Plasmid#77067Purpose3rd generation lentiviral gRNA plasmid targeting human SLKDepositorInsertSLK (SLK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
SLK gRNA (BRDN0001147519)
Plasmid#77068Purpose3rd generation lentiviral gRNA plasmid targeting human SLKDepositorInsertSLK (SLK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
SLK gRNA (BRDN0001146895)
Plasmid#77069Purpose3rd generation lentiviral gRNA plasmid targeting human SLKDepositorInsertSLK (SLK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
SLK gRNA (BRDN0001145408)
Plasmid#77070Purpose3rd generation lentiviral gRNA plasmid targeting human SLKDepositorInsertSLK (SLK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
flag-hKLF6 (1006)
Plasmid#49488Purposeexpresses human flag tagged KLF6 in mammalian cellsDepositorInsertKFL6 (KLF6 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EFS-BirAopt-GSQ-RBXN-P2A-BLAST
Plasmid#208048PurposeEnables constitutive expression of BirAopt alone; RBXN represents a EcoR1-BsiW1-Xba1-Not1 polylinker; to perform bioE3; selection with blasticidinDepositorInsertBirAopt
UseLentiviralTagsExpressionMutationBirAopt is human-codon-optimized BirA (PMID 15707…PromoterEFSAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
EF1a_KLF6_P2A_Hygro_Barcode
Plasmid#120492PurposeBarcoded lentiviral vector to express KLF6 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertKLF6 (KLF6 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJFA344C7
Plasmid#49236PurposeTET1-catalytic domain (CD) expression vector for TALE-TET1 fusion protein to induce targeted demethylation in mammalian cellsDepositorInsertTALE-TET1CD Backbone (TET1 Synthetic, Human)
UseTALEN; Tale-tet1 expression vectorTagsExpressionMutationPromoterEF1aAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJFA334E9
Plasmid#49237PurposeTET1-catalytic domain (CD) expression vector for TALE-TET1 fusion protein to induce targeted demethylation in mammalian cellsDepositorInsertTET1 tet methylcytosine dioxygenase 1 (TET1 Synthetic, Human)
UseTALEN; Tale-tet1 expression vectorTagsExpressionMutationPromoterEF1aAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
FLAG-FullLength-TET1-CXXC(24)
Plasmid#124398PurposeFull-length TET1 missing zinc finger domain (585-624) used to PiggyBAC rescue the function of TET1/2 DKO cellsDepositorInsertFLAG-FullLength-TET1 with CXXC zinc finger deletion (585-624) (TET1 Human)
UseTagsFlagExpressionMammalianMutationCXXC zinc finger deletion (aa 585-624)PromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJFA274
Plasmid#49239PurposeTET1-catalytic domain (CD) expression vector for TALE-TET1 fusion protein to induce targeted demethylation in mammalian cellsDepositorInsertTET1 tet methylcytosine dioxygenase 1 (TET1 Synthetic, Human)
UseTALEN; Tale-tet1 expression vectorTagsExpressionMutationPromoterEF1aAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
USPL1cat-C236S-pEYFP_C1
Plasmid#85767Purposemammalian expression of USPL1 catalytic domain (aa 212-514) as YFP fusion, catalytic inactive variant C236SDepositorInsertUSPL1, catalytic domain (aa 212-514), catalytic inactive variant C236S (USPL1 Human)
UseTagsEYFPExpressionMammalianMutationcatalytic domain (aa 212-514), catalytic inactiv…PromoterCMVAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
FLAG-FullLength-TET1-LLAA(20)
Plasmid#124397PurposeFull-length mutant TET1 (L897A/L900A) used to PiggyBAC rescue the function of TET1/2 DKO cellsDepositorInsertFLAG-FullLength-TET1 with L897A/L900A mutations (TET1 Human)
UseTagsFlagExpressionMammalianMutationL897A/L900A mutations (full length protein is 213…PromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJFA345D4
Plasmid#49238PurposeTET1-catalytic domain (CD) expression vector for TALE-TET1 fusion protein to induce targeted demethylation in mammalian cellsDepositorInsertTET1 tet methylcytosine dioxygenase 1 (TET1 Synthetic, Human)
UseTALEN; Tale-tet1 expression vectorTagsExpressionMutationPromoterEF1aAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUBQ10::ZF1CACTA1_3xFLAG_TET1cd
Plasmid#106434PurposepUBQ10 driven Zinc Finger human TET1 catalytic domain fusion that targets the CACTA1 promoter in arabidopsisDepositorInsertTET1 tet methylcytosine dioxygenase 1 (TET1 Synthetic, Human)
UseSynthetic BiologyTags3xFLAGExpressionPlantMutationPromoterAvailable sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUBQ10::ZF108_3xFLAG_TET1cd
Plasmid#106432PurposepUBQ10 driven Zinc Finger human TET1 catalytic domain fusion that targets the FWA promoter in arabidopsisDepositorInsertpUBQ10::ZF108_3xFLAG_TET1cd (TET1 Mustard Weed, Synthetic, Human)
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only