We narrowed to 6,558 results for: siae

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p416TEF-SEC16

Plasmid

#102676

Purpose

The gene SEC16 is driven by the strong promoter TEF. Overexpression of SEC16 increases recombinant protein secretion

Depositor

Jens Nielsen

Article

Bao et al Appl Environ Microbiol. 2017 Jun 30;83(1

Insert

SEC16 (SEC16 Budding Yeast)

Expression

Yeast

Promoter

TEF

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pFA6a-TRP1-PGAL1-GFP

Plasmid

#41615

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

GAL1 promoter (GAL1 Budding Yeast)

Use

Yeast genomic targeting

Tags

GFP(S65T), TRP1 reverse orientation, and yeast AD…

Available Since

Dec. 11, 2012

Availability

Academic Institutions and Nonprofits only

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pNC1137

Plasmid

#41563

Depositor

Beverly Errede

Article

Houser et al Yeast. 2012 Dec;29(12):519-30. doi: 10.1

Insert

Ura3-FUS1-UBIMdkGFP*-SpHis5-Tim9

Mutation

GFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…

Available Since

April 2, 2013

Availability

Academic Institutions and Nonprofits only

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pJCSUP35(254-684)

Plasmid

#1092

Depositor

Susan Lindquist

Article

Glover et al Cell 1997 May 30;89(5):811-9.

Insert

SUP35 (SUP35 Budding Yeast)

Tags

His

Expression

Bacterial

Mutation

amino acids 254 - 684 of SUP35

Available Since

Jan. 21, 2005

Availability

Academic Institutions and Nonprofits only
pMM2_12

Plasmid

#183061

Purpose

Control for biosensor in pMM2_9: BM3R1-HAA1-mTurquoise2 under HAA1p and mCherry under HREsYGP1p with flanks for genome integration into HO locus

Depositor

Yvonne Nygård

Article

Mormino et al FEMS Yeast Res. 2021 Sep 11;21(6). pii:

Inserts

mCherry
BM3R1-HAA1-mTurquoise2

Use

Synthetic Biology

Tags

mCherry and mTurquoise2

Expression

Yeast

Promoter

HAA1 and Synthetic promoter

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMM2_10

Plasmid

#183059

Purpose

Control for biosensor in pMM2_9: HAA1-mTurquoise2 under HAA1p and mCherry under HREsYGP1p with flanks for genome integration into HO locus

Depositor

Yvonne Nygård

Article

Mormino et al FEMS Yeast Res. 2021 Sep 11;21(6). pii:

Inserts

mCherry
HAA1-mTurquoise2

Use

Synthetic Biology

Tags

mCherry and mTurquoise2

Expression

Yeast

Promoter

HAA1 and Synthetic promoter

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMM2_11

Plasmid

#183060

Purpose

Control for biosensor in pMM2_9: HAA1-mTurquoise2 under HAA1p and mCherry under HREsENO1cp with flanks for genome integration into HO locus

Depositor

Yvonne Nygård

Article

Mormino et al FEMS Yeast Res. 2021 Sep 11;21(6). pii:

Inserts

mCherry
HAA1-mTurquoise2

Use

Synthetic Biology

Tags

mCherry and mTurquoise2

Expression

Yeast

Promoter

HAA1 and Synthetic promoter

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pJCSUP35(123-254)

Plasmid

#1090

Depositor

Susan Lindquist

Article

Glover et al Cell 1997 May 30;89(5):811-9.

Insert

SUP35 (SUP35 Budding Yeast)

Tags

His

Expression

Bacterial

Mutation

amino acids 123 - 254

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
YIp211-P30-His-pol30(K127/164R)-3'

Plasmid

#99549

Purpose

Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127/164R; URA3 marker

Depositor

Helle Ulrich

Article

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

POL30 (POL30 Budding Yeast)

Tags

6xHis

Expression

Yeast

Mutation

K127R, K164R

Promoter

POL30

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pK3FS-PYK1

Plasmid

#85777

Purpose

N-ICE plasmid pK3FS-PYK1

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-KanMX-loxP-PYK1p-3FLAG

Use

Cre/Lox

Expression

Yeast

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pILGFPQ3

Plasmid

#83558

Purpose

Used to evaluate the expression output of GAL2 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

Insert

GAL2 promoter-yEGFP

Expression

Yeast

Promoter

GAL2

Available Since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pTJK517

Plasmid

#121474

Purpose

CEN TRP1 CNA1

Depositor

Tami Kingsbury

Article

Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

Insert

CNA1

Use

Yeast cen vector

Promoter

Endogenous genomic promoter region

Available Since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-TRP1-PGAL1-GST

Plasmid

#41612

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

GAL1 promoter (GAL1 Budding Yeast)

Use

Yeast genomic targeting

Tags

GST, TRP1 reverse orientation, and yeast ADH1 ter…

Available Since

Dec. 11, 2012

Availability

Academic Institutions and Nonprofits only

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HCKan-P-TEF2

Plasmid

#65337

Purpose

construction of TEF2 promoter in HCKan-P receiving vector

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TEF2 promoter

Use

Synthetic Biology

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pTG005

Plasmid

#71386

Purpose

localization of yeast RNase RMP1 (RNase MRP binding protein) in yeast

Depositor

Mark Schmitt

Article

Gill et al J Cell Biol. 2006 Apr 10;173(1):35-45. E

Insert

RMP1 (RMP1 Budding Yeast)

Tags

GFP

Expression

Yeast

Promoter

ACT1

Available Since

Feb. 5, 2016

Availability

Academic Institutions and Nonprofits only

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YIplac204-TDH3p-CDC19-CYC1t

Plasmid

#216122

Purpose

Yeast integrative vector for constitutive expression of CDC19 at the TRP1 locus

Depositor

Yvonne Nygård

Article

Choi et al Metab Eng. 2024 Jul;84:23-33. doi: 10.10

Insert

Pyruvate kinase 1

Expression

Yeast

Promoter

TDH3 promoter

Available Since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pSKT001

Plasmid

#218594

Purpose

Express degron assay: Degradation tag-YFP-ePTS1

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

YFP

Tags

UbiY degradation tag and ePTS1

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB1357

Plasmid

#218595

Purpose

Express Pex11 and the degron assay: Degradation tag-YFP-ePTS1

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Pex11

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pKS1461

Plasmid

#218587

Purpose

Express peroxisomal Erg12p, Erg8p, and Mvd1p to convert mevalonate to IPP

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Erg12

Tags

ePTS1

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-pADH3-Snf7-4V5-APEX2-URA

Plasmid

#207065

Purpose

Moderate overexpression of Snf7-4V5-APEX2 under ADH3 promoter. Marker for late endosomes. Only partially functional.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Snf7

Tags

4xV5-APEX2

Expression

Yeast

Promoter

pADH3

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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