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pHR-pSFFV-dCas9-SunTag-P2A-BFP-dWPRE

Plasmid

#122151

Purpose

Expresses dCas9-SunTag(24X) fusion and a fluorescent indicator BFP.

Depositor

Clifford Brangwynne

Publication

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

dCas9 fused to SunTag(24x)

Use

Lentiviral

Tags

2xNLS-P2A-BFP and NLS

Expression

Mammalian

Promoter

SFFV

Available since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pHAGE-BRAF-V600E

Plasmid

#116204

Purpose

Lentiviral expression of BRAF V600E

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

V600E

Available since

Jan. 11, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pm-iATPSnFR1.0

Plasmid

#102548

Purpose

membrane displayed ATP sensor for mammalian cells

Depositor

Baljit Khakh

Publication

Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

Insert

iATPSnFR1.0

Expression

Mammalian

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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SGEP

Plasmid

#111170

Purpose

miR-E (miR-30 variant)-based RNAi

Depositor

Johannes Zuber

Publication

Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

Insert

miR-E (miR-30 variant)

Use

Lentiviral

Mutation

WT

Promoter

SFFV

Available since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

24 Articles

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pAAV.GFAP.Cre.WPRE.hGH

Plasmid

#105550

Purpose

AAV expression of Cre from GFAP promoter

Depositor

James M. Wilson

Has service

AAV5 and AAV PHP.eB

Insert

Cre

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

GFAP

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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PB-U6insert-EF1puro

Plasmid

#104537

Purpose

A piggybac vector with puromycin resistance and a U6 promoter that allows cloning of guide RNAs

Depositor

George Church

Publication

Kalhor et al Science. 2018 Aug 9. pii: science.aat980

Type

Empty backbone

Use

CRISPR, Mouse Targeting, and Synthetic Biology ; …

Expression

Mammalian

Promoter

U6

Available since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pX330-EN1201

Plasmid

#92144

Purpose

For transient expression of spCas9-nuclease and a sgRNA targeting the mouse TIGRE acceptor locus

Depositor

Benoit Bruneau

Publication

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

spCas9-nuclease and sgRNA against mouse TIGRE acceptor locus

Expression

Mammalian

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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6-His-MBP-TEV-FnCpf1

Plasmid

#90094

Purpose

Bacterial expression plasmid for protein purification

Depositor

Feng Zhang

Publication

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

FnCpf1 (humanized)

Tags

3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

Expression

Bacterial

Promoter

T7

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pU6-Cj-sgRNA

Plasmid

#89753

Purpose

U6 promoter driven expression of Cj-SgRNA cloned with BsmbI

Depositor

Seokjoong Kim

Publication

Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

Insert

Cj-SgRNA

Use

Sgrna expression under u6 promoter

Promoter

U6

Available since

May 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCK002_U6-Sa-sgRNA(mod)_EFS-SaCas9-2A-Puro_WPRE

Plasmid

#85452

Purpose

Lentiviral vector encoding modified SaCas9 system backbone bearing BsmBI site for new guide RNAs and puromycin selection marker.

Depositor

Aviv Regev

Publication

Singer et al Cell. 2016 Sep 8;166(6):1500-1511.e9. do

Insert

U6-modified-sgRNA-Backbone-EFS-hSaCas9-2xNLS-2A-Puro

Use

CRISPR and Lentiviral

Tags

NLS

Expression

Mammalian

Available since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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CAG-Cas9-T2A-EGFP-ires-puro

Plasmid

#78311

Purpose

Expresses WT SpCas9, EGFP and Puromycin resistance from a CAG promoter.

Depositor

Timo Otonkoski

Publication

Saarimaki-Vire et al Cell Rep. 2017 Apr 11;19(2):281-294. doi

Insert

WT SpCas9-T2A-EGFP-ires-puromycin resistance

Use

CRISPR

Tags

T2A-EGFP-ires-puro

Expression

Mammalian

Promoter

CAG

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pAAVS1-P-CAG-mCh

Plasmid

#80492

Purpose

donor vector for AAVS1 targeting (puromycin selection) and constitutive mCherry expression

Depositor

Knut Woltjen

Publication

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Insert

mCherry

Use

Donor vector (human)

Expression

Mammalian

Promoter

CAG

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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TrkB mEGFP

Plasmid

#83952

Purpose

FRET donor for dynamically measuring TrkB activity in neurons

Depositor

Ryohei Yasuda

Publication

Harward et al Nature. 2016 Sep 28;538(7623):99-103. do

Insert

Tropomyosin related kinase B (Ntrk2 Mouse)

Tags

mEGFP

Expression

Mammalian

Available since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCDNA3 HA C9ORF72 op

Plasmid

#74156

Purpose

Express HA tagged optimized C9ORF72 in mammalian cells

Depositor

Nicolas Charlet-Berguerand

Insert

C9ORF72 (C9orf72 Human, Synthetic)

Tags

HA

Expression

Mammalian

Mutation

optimized sequence

Available since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHRm-GFP(1-10)-VP64-NLS

Plasmid

#70228

Purpose

Expresses GFP(1-10)-VP64-NLS in mammalian cells

Depositor

Bo Huang

Publication

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

GFP(1-10)-VP64-NLS

Use

Lentiviral

Expression

Mammalian

Available since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pYPQ142

Plasmid

#69294

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 2 gRNAs

Depositor

Yiping Qi

Publication

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pC13N-iCAG.copGFP

Plasmid

#66578

Purpose

CLYBL safe harbor gene targeting donor expressing CAG-driven copGFP, compatible with pZT-C13 TALENs

Depositor

Jizhong Zou

Publication

Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

Insert

copGFP

Expression

Mammalian

Promoter

CAG

Available since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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Cas9-NAT

Plasmid

#64329

Purpose

Cas9 expression cassette carried by a yeast single-copy episome plasmid

Depositor

Yong-Su Jin

Publication

Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

Insert

Human-optimized S. pyogenes Cas9 with Clonnat marker gene expression cassette

Use

CRISPR

Expression

Yeast

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pCBC-MT1T2

Plasmid

#50593

Purpose

CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

Depositor

Qi-Jun Chen

Publication

Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

Insert

T1, gRNA scaffold, OsU3t plus, TaU3p, T2

Use

CRISPR; Pcr template

Expression

Plant

Available since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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mTagRFP-Membrane-1

Plasmid

#57992

Purpose

Localization: Plasma Membrane, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Type

Empty backbone

Tags

N-terminal 20 Aas MLS from Neuromodulin and mTagR…

Expression

Mammalian

Available since

Dec. 5, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

6 Articles

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