We narrowed to 5,500 results for: crispr cas9 grna plasmid

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pCENPBD1.1.0-gDNA

Plasmid

#132460

Purpose

CRISPR/Cas9 plasmid to create GFP fusion proteins

Depositor

Kevin White

Insert

CENPBD1 (CENPBD1 Human)

Use

CRISPR

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pTHAP7.1.0-gDNA

Plasmid

#132439

Purpose

CRISPR/Cas9 plasmid to create GFP fusion proteins

Depositor

Kevin White

Insert

THAP7 (THAP7 Human)

Use

CRISPR

Available Since

Dec. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pLSC-5

Plasmid

#62889

Purpose

Lenti vector for expression of inducible split Cas9

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

Inserts

SpCas9(574-1368)
SpCas9(2-573)

Use

CRISPR and Lentiviral

Tags

FFBP12, FRB, NES PTK2, NLS SV40, and P2A

Expression

Mammalian

Mutation

*see comment below

Promoter

EFS

Available Since

March 26, 2015

Availability

Academic Institutions and Nonprofits only

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HCP9

Plasmid

#166111

Purpose

This plasmid encodes a Cas9 protein as well as a sgRNA that targets the C-terminus of Hta2

Depositor

Matthias Heinemann

Insert

Hta2-sg18 (HTA2 Budding Yeast)

Use

CRISPR

Expression

Yeast

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pXPR_050

Plasmid

#96925

Purpose

lentiviral expression of gRNA scaffold

Depositor

John Doench, David Root

Article

Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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rtTA-sg-mGreenLantern332

Plasmid

#188483

Purpose

gRNA plasmid with neomycin resistance expressing a single guide RNA targeting mGreenLantern fluorescent protein site 332.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

mGreenLantern sgRNA

Use

CRISPR

Tags

PB_rtTA_BsmBI

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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rtTA-sg-CMV152

Plasmid

#188485

Purpose

gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 152.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

CMV promoter sgRNA

Use

CRISPR

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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rtTA-sg-CMV162

Plasmid

#188486

Purpose

gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 162.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

CMV promoter sgRNA

Use

CRISPR

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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rtTA-sg-CMV51

Plasmid

#188484

Purpose

gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 51.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

CMV promoter sgRNA

Use

CRISPR

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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rtTA-sg-mGreenLantern/EGFP

Plasmid

#188482

Purpose

gRNA plasmid with neomycin resistance expressing a single guide RNA targeting mGreenLantern and EGFP fluorescent proteins.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

mGreenLantern/EGFP sgRNA

Use

CRISPR

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pNM220_AAVS1 integration helper

Plasmid

#211864

Purpose

Cas9 cutting at AAVS1 safe harbor locus

Depositor

Iain Cheeseman

Insert

AAVS1

Use

CRISPR

Mutation

N/A

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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APq5210

Plasmid

#66086

Purpose

co-expression of Cas9 and a sgRNA targeting K08F4.2 in the middle of the ORF

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APa4-2

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only
AP303-4

Plasmid

#66088

Purpose

co-expression of Cas9 and a sgRNA targeting 5'end of C.elegans K08F4.2

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APs1

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
AP334-5

Plasmid

#66096

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APs5

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
APq5271

Plasmid

#66085

Purpose

co-expression of Cas9 and a sgRNA targeting K08F4.2 in the middle of the ORF

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APa4-2

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
APCSD 54

Plasmid

#66100

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans swan-2

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for CSD 54

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only
APCSD 53

Plasmid

#66099

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans swan-2

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for CSD 53

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only
AP334-6

Plasmid

#66097

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APs5

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only
AP334-2

Plasmid

#66095

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APs5

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only
AP324-4

Plasmid

#66094

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APs6

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only