We narrowed to 20,225 results for: ACE;

1. GB1-A11(2-196, G38R)-Strep

   Plasmid

   #196209

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G38R)-Strep.

   Depositor

   Lalit Deshmukh

   Article

   Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

   Insert

   Annexin A11 (ANXA11 Human)
   

   Use

   Tags

   GB1-6xHis-TEV and Strep-II tag

   Expression

   Bacterial

   Mutation

   G38R

   Promoter

   Available Since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. qTAG-N-Solo-mStayGold-GOLGA2

   Plasmid

   #227325

   Purpose

   Donor template for mStayGold insertion into the N-terminus of the GOLGA2 locus. For Golgi visualization. To be co-transfected with sgRNA plasmid px330-PITCh-GOLGA2 (Addgene #207791)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   GOLGA2 Homology Arms flanking a mStayGold Tag (GOLGA2 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYDS2.0_AT118-H

   Plasmid

   #222846

   Purpose

   Yeast-display plasmid encoding Nb. AT118-H, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R)

   Depositor

   Andrew Kruse

   Article

   Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

   Insert

   AT118-H
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA4/TO/GFP-TTP

   Plasmid

   #141190

   Purpose

   Expresses GFP-TTP in mammalian cells, can be used to make inducible cell line

   Depositor

   Simon Bekker-Jensen

   Article

   Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

   Insert

   TTP (ZFP36 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5-FRT/TO-3*Flag-APEX2 N-term PSPC1

   Plasmid

   #187579

   Purpose

   Express FLAG epitope and APEX2-tagged PSPC1 fusion protein in mammalian cells

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Insert

   PSPC1 (PSPC1 Human)
   

   Use

   Tags

   APEX2-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-Beta2

   Plasmid

   #166772

   Purpose

   Encodes a Gbeta subunit as a "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GNB2 (GNB2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-Beta4

   Plasmid

   #166773

   Purpose

   Encodes a Gbeta subunit as a "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GNB4 (GNB4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET42b-BE3

   Plasmid

   #87437

   Purpose

   Expresses BE3-NLS with an N-terminal His Tag (His6) for bacterial expression

   Depositor

   David Liu

   Article

   Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

   Insert

   BE3 (Apobec1 Rat, Human, Bacillus phage PBS2)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. GB1-A11(2-196, G175R)-Strep

   Plasmid

   #196211

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G175R)-Strep.

   Depositor

   Lalit Deshmukh

   Article

   Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

   Insert

   Annexin A11 (ANXA11 Human)
   

   Use

   Tags

   GB1-6xHis-TEV and Strep-II tag

   Expression

   Bacterial

   Mutation

   G175R

   Promoter

   Available Since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. GB1-A11(2-196, G189E)-Strep

    Plasmid

    #196212

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G189E)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

    Insert

    Annexin A11 (ANXA11 Human)
    

    Use

    Tags

    GB1-6xHis-TEV and Strep-II tag

    Expression

    Bacterial

    Mutation

    G189E

    Promoter

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCEP4-FLAG-TAPBPR

    Plasmid

    #135500

    Purpose

    Mammalian expression of FLAG-tagged TAPBPR

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    TAPBPR (TAPBPL Human)
    

    Use

    Tags

    Luminal FLAG epitope tag and Signal peptide from …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCEP4-myc-HLA-B7

    Plasmid

    #135509

    Purpose

    Mammalian expression of myc-tagged HLA-B*07:02

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-B*07:02 (HLA-B Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAnc80L65

    Plasmid

    #68837

    Purpose

    Adeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct. Note that an improved version is available: Addgene plasmid# 92307. See comments for details

    Depositor

    Luk Vandenberghe

    Article

    Zinn et al Cell Rep. 2015 Jul 28. pii: S2211-1247(1

    Insert

    Ancestral AAV Capsid Anc80L65
    

    Use

    AAV and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Rep2

    Available Since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCXLE-hOCT3/4-shp53-F + mCherry-2A-puro

    Plasmid

    #74947

    Purpose

    Dosage control and tracing of reprogramming episomes

    Depositor

    Ann Zovein

    Article

    Schmitt et al Stem Cell Res Ther. 2017 Jun 5;8(1):132.

    Insert

    Oct3/4 (POU5F1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CaMKII-EGFPKASH

    Plasmid

    #124882

    Purpose

    labelling of neuronal nuclear envelope with EGFP

    Depositor

    Vassilis Pachnis

    Article

    Obata et al Nature. 2020 Feb;578(7794):284-289. doi:

    Insert

    EGFP
    

    Use

    AAV

    Tags

    KASH

    Expression

    Mutation

    Promoter

    CaMKII

    Available Since

    Sept. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5-FRT/TO-3*Flag-APEX2 N-term RNPS1

    Plasmid

    #187574

    Purpose

    Express FLAG epitope and APEX2-tagged RNPS1 fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    RNPS1 (RNPS1 Human)
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1(+)/AUG-DAP5-3XFLAG

    Plasmid

    #127331

    Purpose

    Express 3xFLAG tagged DAP5 protein from AUG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    DAP5 (EIF4G2 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GFP-ataxin-3

    Plasmid

    #89975

    Purpose

    Expresses GFP-tagged ataxin-3

    Depositor

    Nico Dantuma

    Article

    Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

    Insert

    ataxin-3 (ATXN3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPMQAK1-Ptrc-[E*](104)-Cas9-B0015-lacZ-PnrsB-mazF-LVA

    Plasmid

    #190790

    Purpose

    [E*](104) construct. Theophylline inducible Cas9 (S. pyogenes) expression. Two BsaI-sites allow for simultaneous insertion of sgRNA and donor DNA. Includes a nickel-inducible curing system.

    Depositor

    Paul Hudson

    Article

    Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

    Inserts

    Cas9
    mazF-LVA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    LVA

    Expression

    Bacterial

    Mutation

    silent mutations of BpiI-sites in original Cas9 g…

    Promoter

    PnrsB and Ptrc-[E*](104)

    Available Since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCK341

    Plasmid

    #192640

    Purpose

    Expresses dSpRY and MCP-SoxS(R93A/S101A) on p15A-CmR

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

    Inserts

    Sp.pCas9-dSpRY
    BBa_J23107-MCP-SoxS(R93A, S101A)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    SoxS has R93A and S101A mutations and dSpRY has s…

    Promoter

    BBa_J23107 and Sp.pCas9

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLD-puro-2A-rtTA-TcVA

    Plasmid

    #24592

    Depositor

    Jason Moffat

    Article

    Mak et al Mol Cell Proteomics. 2010 May;9(5):811-2

    Insert

    Gateway(TM) cassette
    

    Use

    Lentiviral

    Tags

    VA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. (114) pcDNA3.1-GFP-Flag-JunB-EPEA

    Plasmid

    #160743

    Purpose

    Expresses a GFP tagged JunB with Flag and EPEA tags.

    Depositor

    Christina Woo

    Article

    Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

    Insert

    GFP-Flag-JunB-EPEA (JUN Human)
    

    Use

    Tags

    EPEA, Flag, and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available Since

    May 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pME minprom_creERT2

    Plasmid

    #195966

    Purpose

    Gateway pME CreERT2 ORF

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    mouse beta-globin minimal promoter creERT2
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA5-FRT/TO-3*Flag-APEX2 C-term SP100

    Plasmid

    #187584

    Purpose

    Express FLAG epitope and APEX2-tagged SP100 fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    SP100 (SP100 Human)
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLKO-CreERT2 sgRipk4 v3

    Plasmid

    #158048

    Purpose

    lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA against mouse Ripk4. NO Cas9

    Depositor

    Daniel Schramek

    Article

    Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

    Insert

    Ripk4 sgRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Oct. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pINDUCER20_mStrawberry

    Plasmid

    #161734

    Purpose

    Doxycycline-inducible expression of mStrawberry

    Depositor

    Alec Kimmelman

    Article

    Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:

    Insert

    mStrawberry
    

    Use

    Lentiviral; Destinatioin vector for gateway cloni…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. qTAG-C-moxGFP-Puro-LAMP1

    Plasmid

    #207788

    Purpose

    Donor template for moxGFP-2A-Puro insertion into the C-terminus of the LAMP1 locus. For lysosome visualization.To be co-transfected with sgRNA plasmid px330-LAMP1 (Addgene #207787)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    LAMP1 Homology Arms flanking a moxGFP-Puro Cassette (LAMP1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 1, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pLKO-CreERT2 sgAdam10 v3

    Plasmid

    #158047

    Purpose

    lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA against mouse Adam10. NO Cas9

    Depositor

    Daniel Schramek

    Article

    Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

    Insert

    Adam10 sgRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Oct. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. T7-p53DD-pcDNA3

    Plasmid

    #25989

    Depositor

    William Kaelin

    Article

    Irwin et al Nature. 2000 Oct 5. 407(6804):645-8.

    Insert

    tumor protein p53 (TP53 Human)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    truncated cDNA encodes truncated protein (aa 300-…

    Promoter

    Available Since

    Oct. 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCEP4-myc-HLA-A24

    Plasmid

    #135507

    Purpose

    Mammalian expression of myc-tagged HLA-A*024:02

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-A*24:02 (HLA-A Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge