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Plasmid#61532PurposeExpresses Bmi1 in mammalian cellsDepositorInsertBmi1 (Bmi1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
PTPN13_PDZ_1
Plasmid#103919PurposeProtein expression and purification of PTN13 PDZ1 domainDepositorInsertPTPN13 (PTPN13 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
PTPN13_PDZ_3
Plasmid#103921PurposeProtein expression and purification of PTN13 PDZ3 domainDepositorInsertPTPN13 (PTPN13 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZS1[TreR-T]
Plasmid#60754PurposeContains PI driving expression TreR-T, the Trehalose inducible chimera with the TAN DBD.DepositorInsertsTreR-T
GalS-T
UseSynthetic BiologyTagsExpressionBacterialMutationChimeric LacI/GalR repressor with the TAN DBD and…PromoterAvailable sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCIG-FGFRdn
Plasmid#80431PurposeExpression of a dominant-negative FGFR1 with a nuclear EGFP reporter in chick embryosDepositorInsertFibroblast growth factor receptor 1 (FGFR1 Chicken)
UseTagsEGFPExpressionMammalianMutationcontains aa 1–425Promoterbeta-actin promoterAvailable sinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-VprBP (C590)
Plasmid#133589PurposeExpresses human VprBP C590 mutant in mammalian cellsDepositorInsertVpr (HIV-1) binding protein (DCAF1 Human)
UseTagsHAExpressionMammalianMutationC590 mutation of human VprBP lacking the N-termin…PromoterAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
7749 Bicistronic_ires_hygro
Plasmid#64374PurposeThis a retroviral expression plasmid with a minimal polylinker followed by IRES and hygro resistance geneDepositorTypeEmpty backboneUseRetroviralTagsExpressionMutationPromoterAvailable sinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQCascade-IS3
Plasmid#140626PurposeExpresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS3. The crRNA-IS3 targets IS3 loci in Escherchia coli.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS3
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterAvailable sinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pST_SERF2_HUMAN (pBS0751)
Plasmid#185239PurposeFor the mammalian expression of the human protein SERF2_HUMAN. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).DepositorInsertSERF2_HUMAN
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPKm-230
Plasmid#90499PurposepSIN - EF-1alpha - PIF3 - MTAD - IRES - PhyB - GBD, dual vector of PIF3-MTAD under EF-1 alpha promoter and PhyB-DBD under IRES promoter; See growth conditions below.DepositorInsertmito-tFD and mito-tFNR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLD-hygro-EnVM
Plasmid#24590DepositorInsertGateway(TM) cassette
UseLentiviralTagsVMExpressionMammalianMutationPromoterAvailable sinceJuly 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbleo-PIF3-mEGFP
Plasmid#139483PurposeExpresses PIF3-mEGFP in mammalian cells.DepositorInsertPIF3 (PIF3 Mustard Weed)
UseLentiviralTagsmEGFPExpressionMammalianMutationPIF3 1-100 aaPromoterHuman EF1a promoterAvailable sinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
CAHS2parrc-mCh-2xFKBP (pBS1109)
Plasmid#185304PurposeFor the mammalian expression of the tardigrade protein CAHS2parrc attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertCAHS2parrc
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
PTPN13_PDZ_2
Plasmid#103920PurposeProtein expression and purification of PTN13 PDZ2 domainDepositorInsertPTPN13 (PTPN13 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
PTPN13_PDZ_5
Plasmid#103923PurposeProtein expression and purification of PTN13 PDZ5 domainDepositorInsertPTPN13 (PTPN13 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pST_MAHS_RAMVA (pBS0310)
Plasmid#185169PurposeFor the mammalian expression of the tardigrade protein MAHS_RAMVA. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).DepositorInsertMAHS_RAMVA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX330 NC-FKBP-Cas9
Plasmid#162725PurposeMammalian expressionDepositorInsertFKBP F36V
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLX311 ARHGAP30
Plasmid#117739PurposeOpen reading frame vector encoding ARHGAP30DepositorInsertARHGAP30 (ARHGAP30 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTER GAMMA-TUBULIN shRNA Human
Plasmid#87955Purposereduced the expression of gamma-tubulin in human cellsDepositorInsertsh gamma-tubulin (TUBG1 Human)
UseTagsNo-tagExpressionMammalianMutationThe protein is a sh-gamma-tubulin resistant gene.…PromoterAvailable sinceOct. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-miR-34b/34c
Plasmid#63933PurposeMSCV vector for overexpressing mouse miR-34b/34cDepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-PGKnVA
Plasmid#24589DepositorInsertGateway(TM) cassette
UseLentiviralTagsVAExpressionMammalianMutationPromoterAvailable sinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-Ef1a-mCh-GFPi
Plasmid#31849DepositorInsertGFP RNAi
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterU6 for shRNA; Ef1alpha for mCherryAvailable sinceJan. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-M.Mus.1
Plasmid#196681PurposeRep/Cap plasmid for the production of M.Mus.1, an AAV capsid with CNS tropism in mice.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationWVLPSGG insert between amino acids 588 and 589 of…Promoterp41Available sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRI011-pYFAC-pyroA-PgpdA-4xcrRNAarrayelcA-TtrpC
Plasmid#140203PurposeFungal vector to express an LbCas12a crRNA array targeted to Pelca, to be contransformed with pCRI009 containing PelcA-mCherry in a dLbCas12a-VPR strain as proof-of-concept of CRISPRa.DepositorInsertcrRNA array elcA
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterPgpdAAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNOC_dfnCas12a_crRNA NC
Plasmid#176256PurposepNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged with Nlux and spacer sequence is replaced by the type IIS restriction site for endonuclease BaeI that can beDepositorInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseExpressionMutationD917A and E1006A mutations to inactivate the endo…PromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
CFP-GIV-CT WT
Plasmid#69532PurposeMammalian expression of The CFP-tagged GIV-CT biosensorsDepositorInsertGIV (CCDC88A Human)
UseTagsCFPExpressionMammalianMutationC terminal amino acids S1660-S1870 of human GIVPromoterCMVAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-Ef1a-mCh-Puro-Luci
Plasmid#31850DepositorInsertLuciferase RNAi
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterU6 for shRNA; Ef1alpha for mCherry-puromycinAvailable sinceJan. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBabe_puro_DEST_Flag_PRKCI
Plasmid#45263DepositorInsertPKCI (PRKCI Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceJune 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCK669
Plasmid#192638PurposeExpresses dxCas9-NG and MCP-SoxS(R93A/S101A) on p15A-CmRDepositorInsertsSp.pCas9-dxCas9-NG
BBa_J23107-MCP-SoxS(R93A, S101A)
UseCRISPR and Synthetic BiologyTagsExpressionMutationSoxS has R93A and S101A mutations and dxCas9-NG h…PromoterBBa_J23107 and Sp.pCas9Available sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5_FRB-pBirA-HA
Plasmid#83415Purposeproximity-directed biotin labeling on the FRB domain of mTOR in mammalian cellDepositorInsertFRB-pBirA-HA (MTOR Synthetic, Human)
UseTagsHA and pBirAExpressionMammalianMutationPromoterCMV-FAvailable sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only