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We narrowed to 9,202 results for: sacs

Showing: 7231 - 7260 of 9202 results

  1. pET28a-SdrG-B2-ELP-HIS-ybbR

    Plasmid
    #169134
    Purpose
    E. coli expression of SdrG N2, N3 and B2 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.
    Depositor
    Michael Nash
    Article
    Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi
    Insert
    Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
    Use
    Tags
    3xELP linker, 6x Histag, and ybbr tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    169134-plasmid-map-sequence-id-380322

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  2. pET28a-Fgß-FIVAR-ELP-HIS-ybbR

    Plasmid
    #169136
    Purpose
    E. coli expression of FIVAR domain containing Fgß tag, ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.
    Depositor
    Michael Nash
    Article
    Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi
    Insert
    FIVAR (found in various architectures)
    Use
    Tags
    3xELP linker, 6x Histag, Fgβ peptide, and ybbr ta…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    169136-plasmid-map-sequence-id-361162

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  4. pET28a-SdrG-B1-ELP-HIS-ybbR

    Plasmid
    #169133
    Purpose
    E. coli expression of SdrG N2, N3 and B1 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.
    Depositor
    Michael Nash
    Article
    Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi
    Insert
    Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
    Use
    Tags
    3xELP linker, 6x Histag, and ybbr tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    169133-plasmid-map-sequence-id-361153

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  10. pCfB8156

    Plasmid
    #175200
    Purpose
    Xenopus oocyte expression vector containing yEGFP. Contains T7 RNA polymerase binding site, and adds polyA tail to yEGFP. Used for generation of mRNA, for injection into xenopus laevis oocytes.
    Depositor
    Irina Borodina
    Article
    Moller-Hansen et al Front Microbiol. 2024 Apr 12;15:1376653.
    Insert
    yEGFP
    Use
    Mrna expression, injection into xenopus oocytes
    Tags
    PolyA
    Expression
    Mutation
    Promoter
    T7
    Available since
    Aug. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    175200-plasmid-map-sequence-id-347988

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  11. pEndo-I-OnuI-TSM

    Plasmid
    #207954
    Purpose
    Expresses a fusion of the homing endonuclease I-OnuI with the thermosensitive L212P VMA1 intein. Endonuclease activity is restricted to lower temperatures.
    Depositor
    David Edgell
    Article
    Foo et al Nucleic Acids Res. 2024 Feb 28;52(4):206
    Insert
    Thermosensitive fusion of I-OnuI and the L212P VMA1 intein.
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Leucine 212 changed to proline, leucine 434 in fu…
    Promoter
    Available since
    Aug. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    207954-plasmid-map-sequence-id-412663

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  20. pILGFPEasy9R

    Plasmid
    #218291
    Purpose
    Plasmid-free in-situ promoter cloning and characterisation system in S. cerevisiae, allows the characterisation of a bidirectional promoter using yEGFP and E2-Crimson as reporters.
    Depositor
    Claudia Vickers
    Article
    McDonnell et al bioRxiv 2024.03.26.586389
    Insert
    pURA3>KlURA3>tKlURA3-tPGK1KanMX>tAgTEF-pMAL32>MazF(RPL28i)-E2Crimson>tPDC1-pDDI2>ISceI>tSynth3>ura3(4,191)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218291-plasmid-map-sequence-id-433736

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  21. pToxAmp21R

    Plasmid
    #218287
    Purpose
    A complete plasmid for ToxAmp (Toxin-antitoxin-driven gene amplification) for the expression of AeBlue
    Depositor
    Claudia Vickers
    Article
    Evans et al bioRxiv 2024.01.30.578080
    Insert
    HO(-955, -789)-LoxP>pKlLEU2>KlLEU2>PCRT1>RelB>tPDC1-pTDH3>AeBlue>tSYNth7-ARS712-HO(-731, -264)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218287-plasmid-map-sequence-id-433689

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  22. pToxAmp12

    Plasmid
    #218286
    Purpose
    Multi-copy integration of heterologous genes (AeBlue and RelB) through co-transformation with ToxAmp (toxin-antitoxin-driven gene amplification) modules
    Depositor
    Claudia Vickers
    Article
    Evans et al bioRxiv 2024.01.30.578080
    Insert
    HO(-253, -1)-pCRT1>RelB>tPDC1-pTDH3>AeBlue>tsynth7-ARS712-HO(-731, -264)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218286-plasmid-map-sequence-id-433688

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  23. pToxAmp1

    Plasmid
    #218285
    Purpose
    Amplifying the gene copy number of heterologous genes (yEGFP and RelB) through ToxAmp (toxin-antitoxin-driven gene amplification) mechanism
    Depositor
    Claudia Vickers
    Article
    Evans et al bioRxiv 2024.01.30.578080
    Insert
    HO(-253, -1)-pRPL8B>RelB>tPDC1-pTEF1>yEGFP>tURA3-ARS712-HO(-731, -264)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218285-plasmid-map-sequence-id-433687

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Showing: 7231 - 7260 of 9202 results