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mCherry-MyosinIIC-N-18

Plasmid

#55108

Purpose

Localization: Cytoskeleton, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Article

Ebrahim et al Curr Biol. 2013 Apr 22;23(8):731-6. doi:

Insert

MyosinIIC

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only
pGMEH-T38M-750-luxAB-DAS+4

Plasmid

#49520

Purpose

Hygromycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; constitutively expresses reverse TetR (tetR38); replicates episomally

Depositor

Dirk Schnappinger

Article

Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

Insert

T38M-P750-luxAB-DAS+4

Use

Tags

DAS+4 (AANDENYSENYADAS)

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pTET2-lucCP

Plasmid

#198353

Purpose

Part of Renilla reporter system, encodes Firefly luciferase

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

Firefly Luciferase

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

pTET2

Available since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

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PPAPDC2

Plasmid

#155836

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

PPAPDC2 (PLPP6 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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SMG6

Plasmid

#156020

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

SMG6 (SMG6 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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R

Plasmid

#167170

Purpose

Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-R variant in Sf9 cells

Depositor

Susanne Dobler

Article

Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

Inserts

ATP1A1-R
ATP1B1

Use

Tags

Expression

Bacterial and Insect

Mutation

Promoter

P10 and PH

Available since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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VRQR-HF1 variant

Plasmid

#138563

Purpose

Expresses human codon-optimized SpCas9-VRQR-HF1 and blasticidin resistance: EFS promoter-VRQR-HF1-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

VRQR-HF1

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…

Promoter

EFS

Available since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-ChRmine-eYFP-Kv2.1-WPRE

Plasmid

#130989

Purpose

AAV vector to drive the expression of soma-targeted ChRmine-eYFP under the control of CamKIIa promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Insert

ChRmine

Use

AAV

Tags

EYFP-Kv2.1

Expression

Mutation

Promoter

CaMKIIa

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pMX-Brn4

Plasmid

#47028

Purpose

Expresses mouse Brn4

Depositor

Hans Schöler

Article

Han et al Cell Stem Cell. 2012 Apr 6;10(4):465-72.

Insert

Brn4 (Pou3f4 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

The insert includes 40 bp of the TOPO vector in t…

Promoter

Available since

Aug. 6, 2013

Availability

Academic Institutions and Nonprofits only
pLenti_mpx_AsCas12a(dual-gRNA)_h7SK(PacI-ClaI)_U6(I-SceI-NheI)_PGK-puro

Plasmid

#189635

Purpose

Lentiviral expression of double dual-AsCas12a gRNAs for generating combinatorial AsCas12a 3Cs libraries

Depositor

Manuel Kaulich

Article

Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

Insert

h7SK arrayed Cas12a gRNA cassette, hU6 arrayed Cas12a gRNA cassette, PGK puro cassette

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4.23_TSS1

Plasmid

#210633

Purpose

Luciferase experiment for TAL1 TSS1

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 TSS1 (TAL1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4.23_TSS2

Plasmid

#210634

Purpose

Luciferase experiment for TAL1 TSS2

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 TSS2 (TAL1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4.23_TSS4

Plasmid

#210635

Purpose

Luciferase experiment for TAL1 TSS4

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 TSS4 (TAL1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4.23_TSS5

Plasmid

#210636

Purpose

Luciferase experiment for TAL1 TSS5

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 TSS5 (TAL1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pST1374-dCas9-GCN4

Plasmid

#113026

Purpose

Expresses dCas9-GCN4 in mammalian cells

Depositor

Xingxu Huang

Article

Jiang et al Cell Res. 2018 Jun 6. pii: 10.1038/s4142

Insert

dCas9-GCN4 (GCN4 S. pyogenes, Budding Yeast)

Use

Tags

Expression

Mammalian

Mutation

D10A, H840A, N863A

Promoter

CMV

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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PABPN1L

Plasmid

#155807

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

PABPN1L (PABPN1L Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Elafin

Plasmid

#18102

Depositor

Ronny Drapkin

Article

Labidi-Galy et al Oncogene. 2015 Jan 15;34(3):373-83. doi:

Insert

Elafin (PI3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3.1_SNORD27h

Plasmid

#73066

Purpose

expression clone for human SNORD27 (C/D box snoRNA U27)

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

SNORD27 (SNORD27 Human)

Use

Tags

no tag

Expression

Mammalian

Mutation

expression cassette for SNORD27

Promoter

CMV

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pGMC00013

Plasmid

#172525

Purpose

sgRNA against mouse Mr1

Depositor

Raj Chari

Article

Ruf et al Cancer Immunol Res. 2021 Jun 30. pii: 23

Insert

Mr1 (Mr1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pGMC00014

Plasmid

#172526

Purpose

non targeting sgRNA

Depositor

Raj Chari

Article

Ruf et al Cancer Immunol Res. 2021 Jun 30. pii: 23

Insert

Non-targeting guide

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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