We narrowed to 5,068 results for: codon optimized

1. pYPQ223-STU

   Plasmid

   #138109

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized, catalytically deactivated AsCas12a-SRDX repressor without promoter and terminator

   Depositor

   Yiping Qi

   Article

   Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

   Insert

   dAsCas12a-SRDX
   

   Use

   CRISPR; Gateway compatible cas12a entry clone

   Tags

   NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pABD1275

   Plasmid

   #68017

   Purpose

   PIF1 protein overproduction for purification from bacterial cells

   Depositor

   Bruce Downie

   Article

   Majee et al Proc Natl Acad Sci U S A. 2018 Apr 24;11

   Insert

   PHY-INTERACTING FACTOR 1 (PIF1) (PIL5 Mustard Weed, AT2G20180)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Codon Optimized for bacterial expression (GenScri…

   Promoter

   T7

   Available since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

3. pDONR207 SARS-CoV-2 E 27nt-del_nostop

   Plasmid

   #153955

   Purpose

   Gateway-compatible Entry vector, with insert of E CDS bearing a 27nt deletion from SARS-CoV-2 genomic analysis in Davidson et al. 2020

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   E (E SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector

   Tags

   Expression

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available since

   June 23, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pDONR223 SARS-CoV2 TEV-NSP8

   Plasmid

   #154407

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   NSP8 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector, with insert of n…

   Tags

   Expression

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available since

   July 1, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pDONR221_SLC45A3

   Plasmid

   #132167

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC45A3 (SLC45A3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pCBh-T7-SpCas9-P2A-mTagBFP2(RAS3575)

   Plasmid

   #223111

   Purpose

   pCBh Human expression plasmid for SpCas9 with with C-term BPNLS-P2A-mTagBFP2

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpCas9-P2A-mTagBFP2
   

   Use

   CRISPR

   Tags

   BPNLS-3xFLAG-P2A-mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28b-Hf-RfxCas13d-His

   Plasmid

   #234480

   Purpose

   Plasmid for bacterial expression and purification of High-fidelity RfxCas13d protein (human codon-optimized)

   Depositor

   Miguel Angel Moreno-Mateos

   Article

   Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

   Insert

   RfxCas13d
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Changed four alanine to valine in positions 134, …

   Promoter

   Available since

   April 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. EFS-SpdCas9-Dnmt3A/3L-V3

   Plasmid

   #222500

   Purpose

   Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V3 (R887E and E814G) followed by P2A-mCherry

   Depositor

   Isaac Hilton

   Article

   Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

   Insert

   S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V3 (R887E and E814G) engineered fusion
   

   Use

   Lentiviral

   Tags

   FLAG Tag

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9; R887E and E814…

   Promoter

   EFS

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TRE3GV-SpdCas9-Dnmt3A/3L-V1

   Plasmid

   #222503

   Purpose

   Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V1 (R887E) and hPGK promoter driven mCherry

   Depositor

   Isaac Hilton

   Article

   Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

   Inserts

   S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V1 (R887E) engineered fusion
   mCherry
   

   Use

   Lentiviral

   Tags

   FLAG Tag

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9; R887E in Dnmt3A

   Promoter

   TRE3GV and hPGK

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TRE3GV-SpdCas9-Dnmt3A/3L-V2

    Plasmid

    #222505

    Purpose

    Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V2 (E814G) and hPGK promoter driven mCherry

    Depositor

    Isaac Hilton

    Article

    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

    Inserts

    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V2 (E814G) engineered fusion
    mCherry
    

    Use

    Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; E814G in Dnmt3A

    Promoter

    TRE3GV and hPGK

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TRE3GV-SpdCas9-Dnmt3A/3L-V3

    Plasmid

    #222506

    Purpose

    Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V3 (R887E and E814G) and hPGK promoter driven mCherry

    Depositor

    Isaac Hilton

    Article

    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

    Inserts

    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V3 (R887E and E814G) engineered fusion
    mCherry
    

    Use

    Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; R887E and E814…

    Promoter

    TRE3GV and hPGK

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TRE3GV-SpdCas9-Dnmt3A/3L-catΔ

    Plasmid

    #222508

    Purpose

    Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant catΔ (C706A and R832E) and hPGK promoter driven mCherry

    Depositor

    Isaac Hilton

    Article

    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

    Inserts

    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant catΔ (C706A and R832E) engineered fusion
    mCherry
    

    Use

    Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; C706A and R832…

    Promoter

    TRE3GV and hPGK

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pENTR-CASK

    Plasmid

    #221427

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    CASK (CASK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCG_SARS-CoV1 C-V5-tag ORF7a

    Plasmid

    #179971

    Purpose

    Mammalian expression vector for SARS-CoV-1 ORF7a, V5-tagged. Sequence codon-optimized.

    Depositor

    Konstantin Sparrer

    Article

    Hayn et al Cell Rep. 2021 May 18;35(7):109126.

    Insert

    SARS-CoV-1 ORF7a (ORF7a Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCEP4-myc-S (P.1 lineage / Brazilian variant)

    Plasmid

    #169847

    Purpose

    Mammalian expression plasmid for S of SARS-CoV-2 (P.1 lineage / Brazilian variant) with N-terminal c-myc tag

    Depositor

    Erik Procko

    Article

    Zhang et al Nat Chem Biol. 2022 Mar;18(3):342-351. d

    Insert

    Protein S
    

    Use

    Tags

    HA leader and c-myc tag

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCEP4-myc-S (B.1.1.7 lineage / UK variant)

    Plasmid

    #169848

    Purpose

    Mammalian expression plasmid for S of SARS-CoV-2 (B.1.1.7 lineage / UK variant) with N-terminal c-myc tag

    Depositor

    Erik Procko

    Article

    Zhang et al Nat Chem Biol. 2022 Mar;18(3):342-351. d

    Insert

    Protein S
    

    Use

    Tags

    HA leader and c-myc tag

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pYPQ287-RVR

    Plasmid

    #138124

    Purpose

    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized BsCas12a with N512R, K518V and N522R mutations, without promoter

    Depositor

    Yiping Qi

    Article

    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

    Insert

    BsCas12a RVR variant
    

    Use

    CRISPR; Gateway compatible cas12a entry clone

    Tags

    NLS

    Expression

    Plant

    Mutation

    N512R, K518V and N522R

    Promoter

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pYPQ285-RVR

    Plasmid

    #138121

    Purpose

    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Lb5Cas12a with N512R, K518V and N522R mutations, without promoter

    Depositor

    Yiping Qi

    Article

    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

    Insert

    Lb5Cas12a RVR variant
    

    Use

    CRISPR; Gateway compatible cas12a entry clone

    Tags

    NLS

    Expression

    Plant

    Mutation

    N512R, K518V and N522R

    Promoter

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pYPQ233-STU

    Plasmid

    #138111

    Purpose

    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized, catalytically deactivated LbCas12a-SRDX repressor without promoter and terminator

    Depositor

    Yiping Qi

    Article

    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

    Insert

    dLbCas12a-SRDX
    

    Use

    CRISPR; Gateway compatible cas12a entry clone

    Tags

    NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYPQ239-STU

    Plasmid

    #138112

    Purpose

    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized FnCas12a without promoter and terminator

    Depositor

    Yiping Qi

    Article

    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

    Insert

    FnCas12a
    

    Use

    CRISPR; Gateway compatible cas12a entry clone

    Tags

    NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge