We narrowed to 8,305 results for: gnal

1. HBT-CPK30ac-Flag

   Plasmid

   #107429

   Purpose

   For Arabidopsis mesophyll protoplast transient expression assay

   Depositor

   Jen Sheen

   Article

   Liu et al Nature. 2017 May 18;545(7654):311-316. d

   Insert

   CPK30 (CPK30 Mustard Weed)
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. HBT-CPK30-Flag

   Plasmid

   #107430

   Purpose

   For Arabidopsis mesophyll protoplast transient expression assay

   Depositor

   Jen Sheen

   Article

   Liu et al Nature. 2017 May 18;545(7654):311-316. d

   Insert

   CPK30 (CPK30 Mustard Weed)
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-mCherry-mCherry-FLARE-CKAR

   Plasmid

   #123345

   Purpose

   Red-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   mCherry-mCherry-FLARE-CKAR
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-Cerulean3-Cerulean3-FLARE-AKAR

   Plasmid

   #123335

   Purpose

   Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Cerulean3-Cerulean3-FLARE-AKAR
   

   Use

   Tags

   6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. LIC-Z-delCry2

   Plasmid

   #153545

   Purpose

   this is the non-clustering version of LIC-Z tagged with mcherry

   Depositor

   Katharina Gaus

   Article

   Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:

   Insert

   human TCR ζ-Chain (CD247 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   with Cry2 sequence deleted from LIC-Z

   Promoter

   CMV

   Available Since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRC/CMV-TYK2VF/PA-VSV

   Plasmid

   #139351

   Purpose

   Expression of the double mutant of TYK2-V678F-P1104A

   Depositor

   Sandra Pellegrini

   Article

   Li et al J Immunol. 2013 Mar 1;190(5):2335-44. do

   Insert

   TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
   

   Use

   Tags

   VSV

   Expression

   Mammalian

   Mutation

   V362F-V678F-P1104A

   Promoter

   CMV

   Available Since

   June 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. MEN2A-MAC

   Plasmid

   #139634

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

   Insert

   MEN2A (RET Human)
   

   Use

   Tags

   StrepIII-HA-BirA*

   Expression

   Mammalian

   Mutation

   C634W

   Promoter

   CMV

   Available Since

   May 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. GRB2-SHN

   Plasmid

   #139625

   Purpose

   StrepIII-HA-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

   Insert

   GRB2 (GRB2 Human)
   

   Use

   Tags

   StrepIII-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBB0238-TEV-His12

   Plasmid

   #137038

   Purpose

   expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0238 with C-terminal TEV-protease-cleavable His12

   Depositor

   Debra Hansen

   Article

   Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

   Insert

   AAC66635.2
   

   Use

   Tags

   His12 (TEV protease cleavable)

   Expression

   Bacterial

   Mutation

   wild type, full-length protein, contains signal p…

   Promoter

   T7

   Available Since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. BtGRK2[D110A K220R]-mVenus

    Plasmid

    #137779

    Purpose

    Visualization of GRK2

    Depositor

    Dorus Gadella

    Article

    Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

    Insert

    Bovine GRK2[D110A K220R] (GRK2 Bovine)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D110A K220R

    Promoter

    CMV

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. HsGRK6[L66A E514A]-sYFP2

    Plasmid

    #137781

    Purpose

    Visualization of GRK6

    Depositor

    Dorus Gadella

    Article

    Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

    Insert

    Human GRK6[L66A E514A] (GRK6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L66A E514A

    Promoter

    CMV

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCEP4-HA-Flag-natT1R2 RR837-8GG

    Plasmid

    #113958

    Purpose

    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R2 (TAS1R2 Human)
    

    Use

    Tags

    FLAG and Signal/leader sequence from influenza he…

    Expression

    Mammalian

    Mutation

    residues 22-839, R837G and R838G substitution

    Promoter

    cmv

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. NES-PAmCherry-cPHx1

    Plasmid

    #116859

    Purpose

    PI(3,4)P2 biosensor

    Depositor

    Gerry Hammond

    Article

    Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

    Insert

    PLEKHA1 (PLEKHA1 Human)
    

    Use

    Tags

    PAmCherry

    Expression

    Mammalian

    Mutation

    amino acids 169-329

    Promoter

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGGS_cyt-AKAR5

    Plasmid

    #114138

    Purpose

    Mammalian expression of the AKAR5 sensor tagged with nucleus export signal (NES) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

    Depositor

    Haining Zhong

    Article

    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

    Insert

    AKAR5 with C-terminal nuclear export signal (NES)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA5-puro-eGFP-HEC1-M4

    Plasmid

    #114036

    Purpose

    Expression of HEC1 M4 mutant tagged with GFP

    Depositor

    Geert Kops

    Article

    Hiruma et al Science. 2015 Jun 12;348(6240):1264-7. d

    Insert

    GFP tagged - HEC1 -M4 (NDC80 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M4 (K146E, R153E, K156E)

    Promoter

    CMV TetO

    Available Since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA5-puro-eGFP-HEC1-M2

    Plasmid

    #114035

    Purpose

    Expression of HEC1 M2 mutant tagged with GFP

    Depositor

    Geert Kops

    Article

    Hiruma et al Science. 2015 Jun 12;348(6240):1264-7. d

    Insert

    GFP tagged - HEC1 -M2 (NDC80 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M2 (K81E, N87E, K89E)

    Promoter

    CMV TetO

    Available Since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. KG#84

    Plasmid

    #110878

    Purpose

    Expresses the C. elegans acy--1 P260S gain-of-function cDNA in ventral cord cholinergic motor neurons

    Depositor

    Kenneth Miller

    Article

    Schade et al Genetics. 2005 Feb;169(2):631-49. doi: 1

    Inserts

    unc-17beta promoter
    acy-1 P260S gain-of-function cDNA
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Changed Proline to Serine at amino acid 260

    Promoter

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLU-CMV-Flag-hSesn2 L373A/N376A/D385A/S387A

    Plasmid

    #111789

    Purpose

    Mammalian expression or 3rd generation lentivirus generation of hSesn2

    Depositor

    Jun Hee Lee

    Article

    Kim et al Nat Commun. 2015 Nov 27;6:10025. doi: 10

    Insert

    hSesn2 (SESN2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    L373A/N376A/D385A/S387A

    Promoter

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. KG#81

    Plasmid

    #110876

    Purpose

    Expresses the C. elegans acy-1 P260S gain-of-function cDNA in body wall muscle

    Depositor

    Kenneth Miller

    Article

    Schade et al Genetics. 2005 Feb;169(2):631-49. doi: 1

    Inserts

    myo-3 promoter
    acy-1 P260S gain-of-function cDNA
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Changed Proline to Serine at amino acid 260

    Promoter

    Available Since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. KG#83

    Plasmid

    #110877

    Purpose

    Expresses the C. elegans acy--1 P260S gain-of-function cDNA pan-neuronally

    Depositor

    Kenneth Miller

    Article

    Schade et al Genetics. 2005 Feb;169(2):631-49. doi: 1

    Inserts

    rab-3 promoter
    acy-1 P260S gain-of-function cDNA
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Changed Proline to Serine at amino acid 260

    Promoter

    Available Since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge