We narrowed to 5,692 results for: org

1. M-NMn-VP64

   Plasmid

   #48676

   Purpose

   Mammalian NM-VP64 nuclease-null Cas9 activator expression, human optimized

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   Cas9-VP64, nuclease-null
   

   Use

   CRISPR

   Tags

   NLS and VP64

   Mutation

   nuclease-null

   Promoter

   CMV

   Available Since

   Oct. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CMV-Cas9C-VPR

   Plasmid

   #80933

   Purpose

   Expresses Cas9C-VPR in mammalian cells.

   Depositor

   George Church

   Article

   Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

   Insert

   Cas9C-VPR
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. mTurquoise2-Rab7(WT)

   Plasmid

   #112959

   Purpose

   In vivo visualization of Rab7 (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   Rab7
   

   Tags

   mTurquoise2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. EB3-3xmScarletI-N1

   Plasmid

   #112928

   Purpose

   In vivo visualization of microtuble plus ends (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   EB3
   

   Tags

   3x mScarlet-I

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-SMVP-Cas9N-U6-gRNA M3

   Plasmid

   #80936

   Purpose

   Expresses Cas9N in mammalian cells; expresses gRNA M3 for Mstn cleavage.

   Depositor

   George Church

   Article

   Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

   Insert

   Cas9N
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. ITPKA_3xmScarletI

   Plasmid

   #112960

   Purpose

   In vivo visualization of F-actin (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   ITPKA
   

   Tags

   3x mScarlet-I

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-CT3G-ERP2-MG

   Plasmid

   #175501

   Purpose

   All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   TRE3G-mCMV

   Available Since

   Oct. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. ITPKA-mScarlet-I

   Plasmid

   #98829

   Purpose

   In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   ITPKA
   

   Tags

   mScarlet-I

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. dSp-VP64-p65(100-261)-RTA(125-190)

   Plasmid

   #99673

   Purpose

   Expresses dSp Cas9 fused to combination of truncated activation domains

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9
   

   Tags

   VP64-p65(101-261)-RTA(125-190)

   Expression

   Mammalian

   Mutation

   dead Cas9

   Available Since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-SMVP-Cas9N-U6-gRNA P1

    Plasmid

    #80943

    Purpose

    Expresses Cas9N in mammalian cells; expresses gRNA P1 for Pd-l1 binding.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9N
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPB-cT3G-cERP2-TERF1

    Plasmid

    #222542

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of TERF1

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    TERF1 (TERF1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-EFS-dSa-VPR mini.

    Plasmid

    #99686

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from EFS promoter

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Use

    AAV

    Tags

    VPR mini

    Mutation

    dead Cas9

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-mNoxo1

    Plasmid

    #58338

    Purpose

    expresses mouse Noxo1 in mammalian cells

    Depositor

    Karl-Heinz Krause, Botond Banfi

    Article

    Banfi et al J Biol Chem. 2003 Feb 7;278(6):3510-3. E

    Insert

    NADPH oxidase organizer 1 (Noxo1 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. M-ST1n-VP64

    Plasmid

    #48675

    Purpose

    Mammalian ST1-VP64 nuclease-null Cas9 activator expression, human optimized

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Cas9-VP64, nuclease-null
    

    Use

    CRISPR

    Tags

    NLS and VP64

    Mutation

    nuclease-null (D9A D598A H599A N622A)

    Promoter

    CMV

    Available Since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CMV-dSa-VPR mini.-2X snRP1

    Plasmid

    #99688

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains with 34 nt dual snRP1 poly adenylation signal

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Use

    AAV

    Tags

    VPR mini

    Mutation

    dead Cas9

    Available Since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1(+) prME (JEV) sdm2

    Plasmid

    #52881

    Purpose

    expression of Japanese Encephalitis Virus VLPs in mammalian cells

    Depositor

    Yorgo Modis

    Article

    Nour et al PLoS Pathog. 2013;9(9):e1003585. doi: 10

    Insert

    prME (POLY Japanese encephalitis virus)
    

    Expression

    Mammalian

    Mutation

    sdm2

    Promoter

    CMV

    Available Since

    May 14, 2014

    Availability

    Academic Institutions and Nonprofits only

17. 3xnls-G-Ca-FLITS

    Plasmid

    #191463

    Purpose

    Genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change

    Depositor

    Dorus Gadella

    Article

    van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

    Insert

    G-Ca-FLITS
    

    Tags

    3xNLS

    Expression

    Mammalian

    Available Since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB-CT3G-CERP2

    Plasmid

    #175499

    Purpose

    All-in-one piggyBac transposon vector for mCMV+dox-inducible expression of Golden Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE3G-mCMV

    Available Since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. gRNA_tdtomato_reporter1_SP-2x

    Plasmid

    #79370

    Purpose

    Assays activity of transcriptional activators fused to SP Cas9

    Depositor

    George Church

    Article

    Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

    Insert

    tdtomato
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    April 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET23a-HsRPS27a

    Plasmid

    #69561

    Purpose

    Bacterial expression; in vitro transcription/translation (T7)

    Depositor

    Jorge Eduardo Azevedo

    Article

    Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

    Insert

    RPS27A (RPS27A Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 11, 2015

    Availability

    Academic Institutions and Nonprofits only