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We narrowed to 27,285 results for: Tat

Showing: 9721 - 9760 of 27285 results

  2. pLCHKO_SFRS7_exon_deletion_1

    Plasmid
    #155069
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Jason Moffat, Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    155069-plasmid-map-sequence-id-308589

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  3. pLCHKO_MDM4_exon_deletion_1

    Plasmid
    #155073
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Jason Moffat, Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    155073-plasmid-map-sequence-id-308586

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  4. pLCHKO_Ptbp_ex8_1

    Plasmid
    #155081
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Jason Moffat, Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155081-plasmid-map-sequence-id-308533

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  5. pLCHKO_Ptbp_ex8_4

    Plasmid
    #155084
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Jason Moffat, Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155084-plasmid-map-sequence-id-308527

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  6. pLCHKO_SFRS7_exon_deletion_4

    Plasmid
    #155072
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Jason Moffat, Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155072-plasmid-map-sequence-id-308512

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  7. pLCHKO_PDPR_exon_deletion_3

    Plasmid
    #155067
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Jason Moffat, Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155067-plasmid-map-sequence-id-308446

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  8. pLCHKO_PDPR_exon_deletion_4

    Plasmid
    #155068
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Jason Moffat, Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155068-plasmid-map-sequence-id-308444

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  9. pET28a-ybbr-XMod-Doc (BMB-KO, S199AzF)-HIS

    Plasmid
    #153445
    Purpose
    E. coli expression (amber suppression) of Rc. XDocB binding mode B knock-out mutant with serine at position 199 replaced with amber codon.
    Depositor
    Michael Nash
    Article
    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:
    Insert
    Rc.XDocB
    Use
    Tags
    6xHis and ybbr tag
    Expression
    Bacterial
    Mutation
    Serine 199 was mutated to amber codon (TAG). Argi…
    Promoter
    T7 promoter
    Available since
    July 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    153445-plasmid-map-sequence-id-306484

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  10. pET28a-ybbr-XMod-Doc (BMA-KO, S199AzF)-HIS

    Plasmid
    #153444
    Purpose
    E. coli expression (amber suppression) of Rc. XDocB binding mode A knock-out mutant with serine at position 199 replaced with amber codon.
    Depositor
    Michael Nash
    Article
    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:
    Insert
    Rc.XDocB
    Use
    Tags
    6xHis and ybbr tag
    Expression
    Bacterial
    Mutation
    Serine 199 was mutated to amber codon (TAG). Argi…
    Promoter
    T7 promoter
    Available since
    July 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    153444-plasmid-map-sequence-id-306456

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  11. B-HypaSpCas9

    Plasmid
    #126763
    Purpose
    Expression plasmid for human codon-opt. increased fidelity Blackjack-HypaSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than HypaSpCas9.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    B-HypaSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N692A; M694A; Q695A; H698A; amino acids 1005-1013…
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126763-plasmid-map-sequence-id-410968

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  12. pET-FLAG-B-eSpCas9

    Plasmid
    #126772
    Purpose
    Expression of increased fidelity Blackjack-eSpCas9 in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of eSpCas9
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    B-eSpCas9
    Use
    CRISPR
    Tags
    3xFLAG, 6xHis, MBP, and NLS
    Expression
    Bacterial
    Mutation
    K848A; K1003A; R1060A; amino acids 1005-1013 repl…
    Promoter
    T7
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126772-plasmid-map-sequence-id-410600

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  13. AdMKP-3 S159AS197A

    Plasmid
    #131523
    Purpose
    Expresses MKP-3 with S159A mutation and S197A mutation in mammalian cells
    Depositor
    Haiyan Xu
    Article
    Feng et al Mol Cell Endocrinol. 2012 Sep 25;361(1-2
    Insert
    Mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
    Use
    Adenoviral
    Tags
    No
    Expression
    Mutation
    changed Serine 159 to Alanine and Serine 197 to A…
    Promoter
    CMV
    Available since
    Oct. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    131523-plasmid-map-sequence-id-256915

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  16. pGEX-UbcH7(C86K)-10aa-linker-delta 1-29 and 117-160 Bactrocera parkin(C463A)

    Plasmid
    #128383
    Purpose
    expresses protein UbcH7 (C86K) fused to Bactrocera parkin (C463A) with residues 1-29 and 117-160 deleted
    Depositor
    Kalle Gehring
    Article
    Sauve et al Nat Struct Mol Biol. 2018 Jul;25(7):623-
    Insert
    UbcH7 (UBE2L3 Human)
    Use
    Tags
    Bactrocera dorsalis parkin with mutation C463A an…
    Expression
    Bacterial
    Mutation
    mutation C86K in UbcH7 gene
    Promoter
    lac promotor
    Available since
    Aug. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    128383-plasmid-map-sequence-id-254044

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  28. MSCV-PIG-N3FLAG-TAF12

    Plasmid
    #105593
    Purpose
    retrovirally express mouse TAF12 with 3*FLAG tag at N terminal, GFP marker and puro resistance
    Depositor
    Christopher Vakoc
    Article
    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.
    Insert
    full length TAF12 with silent mutations, making it resistant to TAF12 shRNA#364 (Taf12 Mouse)
    Use
    Retroviral
    Tags
    3*FLAG
    Expression
    Mammalian
    Mutation
    silent mutations to make it resistant to the targ…
    Promoter
    MSCV-LTR
    Available since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    105593-plasmid-map-sequence-id-209432

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  37. pMCh-623

    Plasmid
    #92266
    Purpose
    Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 6W6A with six mutated W-motifs in its N-terminal domain in D. melanogaster cells
    Depositor
    Witold Filipowicz, Marina Chekulaeva
    Article
    Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1
    Insert
    dGW182 6W6A (gw Fly)
    Use
    Tags
    NHA
    Expression
    Insect
    Mutation
    The following point mutations were introduced W23…
    Promoter
    Ac5
    Available since
    Dec. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    92266-plasmid-map-sequence-id-194528

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Showing: 9721 - 9760 of 27285 results