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Showing: 4861 - 4880 of 8371 results

  1. Ec dnaN-pHK/P

    Plasmid
    #237239
    Purpose
    over-express E. coli hk/P-dnaN (Pol III beta sliding clamp subunit, N-terminal cleveable his-kinase tag)
    Depositor
    Michael O'Donnell
    Insert
    dnaN (beta), N-terminal his-kinase cleavable tag (dnaN E. coli)
    Use
    Tags
    his-10+PKA kinase motif+PreScission Protease clea…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    237239-plasmid-map-sequence-id-469256

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  2. pRDA_852

    Plasmid
    #185527
    Purpose
    Lentiviral vector that enables Cre-mediated expression of two sgRNAs. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.
    Depositor
    Arlene Sharpe
    Inserts
    First sgRNA cassette
    Second sgRNA cassette
    Use
    CRISPR, Cre/Lox, and Lentiviral ; Flp/frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human U6 and Mouse U6
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    185527-plasmid-map-sequence-id-366261

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  3. pdCas9-tdTomato

    Plasmid
    #232631
    Purpose
    catalytically inactive dCas9 for localization; labeled with tdTomato
    Depositor
    Karsten Rippe
    Insert
    dCas9-tdTomato
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV (TATA box removed)
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232631-plasmid-map-sequence-id-462150

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  4. 1996_pCCLsin.PPT.hPGK.EGFP-mCEP83.PRE

    Plasmid
    #231805
    Purpose
    Label the mother centrosome-specific distal appendage protein CEP83 by electroporating an mRNA encoding it as well as an eGFP fusion protein.
    Depositor
    Annette Oxenius
    Insert
    eGFP-mCEP83
    Use
    Lentiviral
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    231805-plasmid-map-sequence-id-460689

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  6. pX330-MM002-ZFP143-C-terminal-sgRNA

    Plasmid
    #212706
    Purpose
    Plasmid for transient expression of pspCas9(BB)-2A-Puro nuclease and sgRNA targetting the C terminal of Zfp143 locus
    Depositor
    Elzo de Wit
    Insert
    ZFP143 C-terminal sgRNA (Zfp143 Mouse)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    212706-plasmid-map-sequence-id-438797

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  7. pENTR/hBIG1

    Plasmid
    #226251
    Purpose
    Entry plasmid of hBIG1 for Gateway system
    Depositor
    Hye-Won Shin
    Insert
    BIG1 (ARFGEF1 Human)
    Use
    Entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    226251-plasmid-map-sequence-id-454235

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  8. pENTR/HA-hBIG2

    Plasmid
    #226252
    Purpose
    Entry plasmid of N-terminally HA-tagged hBIG2 for Gateway system
    Depositor
    Hye-Won Shin
    Insert
    BIG2 (ARFGEF2 Human)
    Use
    Entry vector
    Tags
    HA
    Expression
    Mutation
    Promoter
    Available since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    226252-plasmid-map-sequence-id-454237

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  9. pHR_5xUAS-BFP-WPRE_PGK-HygR-bGHpolyA

    Plasmid
    #216665
    Purpose
    Expresses Gal4 inducible BFP, with hygromycin resistance
    Depositor
    Leonardo Morsut
    Inserts
    BFP
    HygromycinR
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    216665-plasmid-map-sequence-id-432189

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  11. pDGB3_alpha1_pNOS-Rep-RepA-Tnos (GB3598)

    Plasmid
    #225432
    Purpose
    Transcriptional unit of BeYDV Rep/RepA (C1:C2)
    Depositor
    Diego Orzaez
    Insert
    pNOS:Rep/RepA:tNOS
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    225432-plasmid-map-sequence-id-447739

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  12. pEN_TT DNM1L-V5

    Plasmid
    #220359
    Purpose
    Gateway entry vector for an inducible V5-tagged DNM1L
    Depositor
    Kevin Janes
    Insert
    DNM1L (DNM1L Human)
    Use
    Entry vector for gateway cloning
    Tags
    V5
    Expression
    Mutation
    Promoter
    Available since
    Oct. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    220359-plasmid-map-sequence-id-447351

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  13. pET-UBE3A iso 2 N terminus

    Plasmid
    #185643
    Purpose
    expression of His-tagged UBE3A isoform 2 1-87 in bacterial cells
    Depositor
    Irina Bezsonova
    Insert
    ubiquitin-protein ligase E3A (UBE3A Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    none
    Promoter
    Available since
    Sept. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    185643-plasmid-map-sequence-id-367329

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  14. pCDNA3.SLC17A2-flag

    Plasmid
    #224419
    Purpose
    Expresses SLC17A2 in mammalian cells
    Depositor
    Jonathan Long
    Insert
    Solute carrier family 17 (sodium phosphate), member 2 (SLC17A2 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    224419-plasmid-map-sequence-id-444193

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  15. CD55-Delta3

    Plasmid
    #220872
    Purpose
    Expresses CD55 variant in mammalian cells
    Depositor
    Ferenc Scheeren
    Insert
    CD55 Delta-3
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Delta-3, short consensus repeat (SCR) domains 1, …
    Promoter
    CMV
    Available since
    Aug. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    220872-plasmid-map-sequence-id-443043

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  16. CD55-Delta4

    Plasmid
    #220873
    Purpose
    Expresses CD55 variant in mammalian cells
    Depositor
    Ferenc Scheeren
    Insert
    CD55 Delta-4
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Delta-4, short consensus repeat (SCR) domains 1, …
    Promoter
    CMV
    Available since
    Aug. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    220873-plasmid-map-sequence-id-443042

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  17. CD55-Delta1

    Plasmid
    #220870
    Purpose
    Expresses CD55 variant in mammalian cells
    Depositor
    Ferenc Scheeren
    Insert
    CD55 Delta-1
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Delta-1, the first short consensus repeat (SCR) d…
    Promoter
    CMV
    Available since
    Aug. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    220870-plasmid-map-sequence-id-443041

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  18. CD55-Delta2

    Plasmid
    #220871
    Purpose
    Expresses CD55 variant in mammalian cells
    Depositor
    Ferenc Scheeren
    Insert
    CD55 Delta-2
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Delta-2, short consensus repeat (SCR) domains 1 a…
    Promoter
    CMV
    Available since
    Aug. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    220871-plasmid-map-sequence-id-443038

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  19. hs-cre::EBD, UAS-dBrainbow attB

    Plasmid
    #220326
    Purpose
    expresses a heat-shock inducible cre::EBD fusion protein and UAS-dBrainbow
    Depositor
    Erdem Bangi
    Inserts
    Cre::EBD (with intron)
    dBrainbow
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    UAS and hs
    Available since
    Aug. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    220326-plasmid-map-sequence-id-441806

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  20. pCMV-PE2-P2A-PuroR

    Plasmid
    #196962
    Purpose
    Expresses PE2 with puromycin resistance
    Depositor
    Leopold Parts
    Insert
    PE2-P2A-Puro
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    196962-plasmid-map-sequence-id-440213

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Showing: 4861 - 4880 of 8371 results