We narrowed to 5,692 results for: org

1. pSN007

   Plasmid

   #102440

   Purpose

   For PCR amplification to create paired gRNAs to clone into Golden Gate gRNA expression vectors (e.g. pSB700 Plasmid #64046)

   Depositor

   George Church, Alejandro Chavez

   Article

   Nageshwaran et al J Vis Exp. 2018 Oct 2;(140). doi: 10.379

   Insert

   7SK paired gRNA insert
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHRdSV40_scFv_GCN4_sfGFP-VPR-GB1_NLS

   Plasmid

   #79373

   Purpose

   Recruits VPR to a compatible Cas9 protein for transcriptional activation

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   GCN4-sfGFP-VPR
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Oct. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-hNoxo1

   Plasmid

   #58530

   Purpose

   expresses human Noxo1 in mammalian cells

   Depositor

   Karl-Heinz Krause, Botond Banfi

   Article

   Banfi et al J Biol Chem. 2003 Feb 7;278(6):3510-3. E

   Insert

   NADPH oxidase organizer 1 (NOXO1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHRdSV40_scFv_GCN4_sfGFP_p65-hsf1_GB1_NLS

   Plasmid

   #79372

   Purpose

   Recruits p65-hsf1 to a compatible Cas9 protein for transcriptional activation

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   GCN4-sfGFP-p65-hsf1
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   March 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. dABE

   Plasmid

   #124447

   Purpose

   Expresses dead Cas9 (dCas9)-ABE7.10

   Depositor

   George Church

   Article

   Smith et al Nucleic Acids Res. 2020 May 21;48(9):518

   Insert

   dABE7.10
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Available Since

   April 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. EB3-mTurquoise2

   Plasmid

   #98825

   Purpose

   In vivo visualization of microtuble plus ends (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   EB3
   

   Tags

   mTurquoise2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-SMVP-Cas9N-U6-gRNA P2

   Plasmid

   #80944

   Purpose

   Expresses Cas9N in mammalian cells; expresses gRNA P2 for Pd-l1 binding

   Depositor

   George Church

   Article

   Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

   Insert

   Cas9N
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. mTurquoise2-PXN

   Plasmid

   #112957

   Purpose

   In vivo visualization of focal adhesions (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   PXN
   

   Tags

   mTurquoise2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRK010

   Plasmid

   #69874

   Purpose

   expression of Channelrhodopsin-2 XXL (extra high expression and long open state, D156C) mutant

   Depositor

   Robert Kittel, Georg Nagel

   Article

   Dawydow et al Proc Natl Acad Sci U S A. 2014 Sep 23;11

   Insert

   Channelrhodopsin-2
   

   Use

   Unspecified

   Mutation

   D156C (XXL)

   Promoter

   Drosophila Hsp70

   Available Since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLPC31

    Plasmid

    #209996

    Purpose

    Contains Level 0 Part: 3' Connector (3C2SN) for the construction of Level 1 plasmids

    Depositor

    Georg Fritz, Thorsten Mascher

    Article

    Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

    Insert

    3' Connector (3C2SN)
    

    Expression

    Bacterial

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLPC32

    Plasmid

    #209997

    Purpose

    Contains Level 0 Part: 3' Connector (3C5CSN) for the construction of Level 1 plasmids

    Depositor

    Georg Fritz, Thorsten Mascher

    Article

    Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

    Insert

    3' Connector (3C5CSN)
    

    Expression

    Bacterial

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLPC52

    Plasmid

    #209991

    Purpose

    Contains Level 0 Part: 5' Connector (5C2SN) for the construction of Level 1 plasmids

    Depositor

    Georg Fritz, Thorsten Mascher

    Article

    Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

    Insert

    5' Connector (5C2SN)
    

    Expression

    Bacterial

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLPC51

    Plasmid

    #209990

    Purpose

    Contains Level 0 Part: 5' Connector (5C1CSN) for the construction of Level 1 plasmids

    Depositor

    Georg Fritz, Thorsten Mascher

    Article

    Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

    Insert

    5' Connector (5C1CSN)
    

    Expression

    Bacterial

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. EMTB-mTurquoise2

    Plasmid

    #137803

    Purpose

    Visualization of microtubules

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    Ensconsin microtubule binding domain
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSMP-YY1_1

    Plasmid

    #36357

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    YY1 (YY1 Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pSMP-Suv39H1_1

    Plasmid

    #36342

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    Suv39H1 (SUV39H1 Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pRSV-p65T305A

    Plasmid

    #106454

    Purpose

    NF-kB p65, kinase site 305 mutated

    Depositor

    Georg Weber

    Article

    He et al Eur J Biochem. 2003 May . 270(10):2174-8

    Insert

    NF-kB p65, kinase site 305 mutated (RELA Human)
    

    Expression

    Mammalian

    Mutation

    kinase site inactivated

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSMP-YY1_2

    Plasmid

    #36358

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    YY1 (YY1 Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pHJ17

    Plasmid

    #48356

    Purpose

    Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Hygromycin/Gancyclovir selectable

    Depositor

    George Cross

    Article

    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

    Insert

    Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-HYG-Ty1-TK-loxP-3' ALD UTR
    

    Use

    Cre/Lox; Knockout in t. brucei

    Available Since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPB-cT3G-cERP2-MEIOC

    Plasmid

    #222562

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of MEIOC

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    MEIOC (C17orf104 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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