We narrowed to 10,454 results for: ESP

1. pJRH056

   Plasmid

   #171637

   Purpose

   Plasmid containing a U6 promoter expressing a spyCas9 tdTom sgRNA298 and CAG promoter expressing mTagBFP2

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-tdTomato
   mTagBFP2
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   CAG and U6

   Available Since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAG306-GPD-VMA1 chr I

   Plasmid

   #41896

   Purpose

   Yeast expression of VMA1.

   Depositor

   Dan Gottschling

   Article

   Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

   Insert

   VMA1 (VMA1 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   Contains silent mutation at 342A-G and L87S mutat…

   Promoter

   GPD

   Available Since

   April 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRK Smad2 deltaC Flag

   Plasmid

   #12624

   Depositor

   Rik Derynck

   Article

   Zhang et al Nature. 1996 Sep 12. 383(6596):168-72.

   Insert

   Smad2 deltaC (SMAD2 Human)
   

   Tags

   flag

   Expression

   Mammalian

   Mutation

   C-terminal truncated Smad2 (aa 1-424)

   Available Since

   Oct. 10, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pED-hIRE1(I642G)

   Plasmid

   #16263

   Depositor

   Peter Walter

   Article

   Lin et al Science. 2007 Nov 9. 318(5852):944-9.

   Insert

   IRE1 (ERN1 Human)
   

   Expression

   Mammalian

   Mutation

   isoleucine to glycine mutation at residue 642

   Available Since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV U6-sgRNA UbC-tdTomato

   Plasmid

   #106949

   Purpose

   sgRNA cloning cassette using BsmBI/Esp3I enzymes with tdTomato marker

   Depositor

   Albert Edge

   Article

   Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

   Insert

   sgRNA BsmBI/Esp3I cloning site
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. 3462 pSFFV-neo Bcl-2

   Plasmid

   #8776

   Depositor

   Stanley Korsmeyer

   Article

   Zha et al Cell 1996 Nov 15;87(4):619-28.

   Insert

   Bcl-2 (Bcl2 Mouse)
   

   Expression

   Mammalian

   Available Since

   July 18, 2006

   Availability

   Academic Institutions and Nonprofits only

7. Ub[9]-mRFP[1]

   Plasmid

   #20249

   Depositor

   Nico Dantuma

   Article

   Salomons et al Mol Cell Biol. 2009 Apr;29(7):1774-85. d

   Insert

   ubiquitin C (UBC Human)
   

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   changed Stop codon 686 to Glycine

   Available Since

   April 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pVAX_CBI_AcrIIA2

   Plasmid

   #136661

   Purpose

   Expresses human codon-optimized AcrIIA2 in mammalian cells. ORF flanked by NotI and XhoI restriction sites

   Depositor

   Yannick Doyon

   Article

   Hynes et al Nat Commun. 2018 Jul 25;9(1):2919. doi:

   Insert

   AcrIIA2
   

   Use

   Synthetic Biology

   Tags

   No tag, No NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGBW-m4136458

   Plasmid

   #149531

   Purpose

   Bacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable …

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   May 13, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pCGN-ELK-1 S383A

    Plasmid

    #27160

    Purpose

    For mammalian expression of human Elk-1 with an HA tag and a mutation in the MAPK phosphorylation site of the transcriptional activation domain

    Depositor

    Ron Prywes

    Article

    Lee et al J Biol Chem. 2010 Jul 16. 285(29):22036-

    Insert

    ELK1 (ELK1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    TAD mutant: S383A

    Available Since

    Jan. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pXD70DA9-Pct5-DadhABC(A2)

    Plasmid

    #191632

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    Dopamine dehydroxylase
    

    Expression

    Bacterial

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pETDuet RagA(D130N)-RagC

    Plasmid

    #99642

    Purpose

    Bacterial expression of RagA(D130N)-RagC

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    D130N

    Available Since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pETDuet RagA(T21N)-RagC

    Plasmid

    #99644

    Purpose

    Bacterial expression of RagA(T21N)-RagC

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    T21N

    Available Since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-GFAP-2xNLS-mTurquoise2

    Plasmid

    #104053

    Purpose

    An AAV genome encoding expression of the nuclear localized fluorescent protein mTurquoise2 from the GFAP promoter

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Insert

    2xNLS-mTurquoise2
    

    Use

    AAV

    Tags

    NLS

    Promoter

    GFAP

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQCXIP-LIP

    Plasmid

    #15714

    Depositor

    Joan Massague

    Article

    Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

    Insert

    LIP (CEBPB Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Sept. 7, 2007

    Availability

    Academic Institutions and Nonprofits only

16. plko.1/shMCT4 #474

    Plasmid

    #99597

    Purpose

    Knockdown of MCT4 expression

    Depositor

    Eli Bar

    Article

    Lim et al Oncogene. 2014 Aug 28;33(35):4433-41. do

    Insert

    MCT4 (SLC16A3 Human)
    

    Use

    Lentiviral

    Available Since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. WNV NS2B-NS3 protease (K104A - catalytically active, don't self cleave); aliases: West Nile Virus NS2B-NS3 fusion protein

    Plasmid

    #204794

    Purpose

    Bacterial expression of a fusion of West Nile NS2B-NS3 proteins

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    West Nile NS2B-NS3 fusion
    

    Expression

    Bacterial

    Available Since

    Aug. 29, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pETDuet RagA-RagC(D181N)

    Plasmid

    #99643

    Purpose

    Bacterial expression of RagA-RagC(D181N)

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    D181N

    Available Since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBABE puro Brca1 S1423A S1524A HA

    Plasmid

    #41968

    Depositor

    Stephen Elledge

    Article

    Cortez et al Science. 1999 Nov 5. 286(5442):1162-6.

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S1423A, S1524A

    Available Since

    April 26, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA_RGG-RFP-RGG

    Plasmid

    #124934

    Purpose

    Expression of RGG-RFP-RGG

    Depositor

    Matthew Good, Daniel Hammer, Benjamin Schuster

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    RGG-RFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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