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pAAV-VTKD5-GFP

Plasmid

#178710

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of GFP in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

Depositor

Jordane Dimidschstein

Publication

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

GFP

Use

AAV

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKD6-GFP

Plasmid

#178711

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of GFP in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

Depositor

Jordane Dimidschstein

Publication

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

GFP

Use

AAV

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pOTTC1270 - pAAV Rosa26 gRNA A+B EF1a EGFP

Plasmid

#113156

Purpose

An AAV vector that expresses guide RNAs targeting rat Rosa26 and expresses EGFP reporter

Depositor

Brandon Harvey

Publication

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

Two gRNAs for rat Rosa26

Use

AAV and CRISPR

Expression

Mammalian

Promoter

mU6 and hU6

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-AP.Adamts2.1-DIO-GCaMP8s-WPRE

Plasmid

#211491

Purpose

conditional expression of GCaMP8s under artificial promoter Adamts2

Depositor

Georg Keller

Publication

O'Toole et al Neuron. 2023 Sep 6:S0896-6273(23)00626-8

Insert

DIO-GCaMP8s

Use

AAV

Expression

Mammalian

Available since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-AP.Baz1a.1-DIO-GCaMP8s-WPRE

Plasmid

#211493

Purpose

conditional expression of GCaMP8s under artificial promoter Baz1a

Depositor

Georg Keller

Publication

O'Toole et al Neuron. 2023 Sep 6:S0896-6273(23)00626-8

Insert

DIO-GCaMP8s

Use

AAV

Expression

Mammalian

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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MsACR1-TS-Kv-P2A-mCerulean3-pAAV-CaMKII

Plasmid

#204970

Purpose

Soma-targeted neuronal expression of MsACR1 and mCerulean3 separated by a P2A site.

Depositor

Peter Hegemann

Publication

Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

Insert

MsACR1

Use

AAV

Tags

mCerulean3

Promoter

CaMKII

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-smURFP-P2A-GFP

Plasmid

#197151

Purpose

Expresses the protein of smURFP-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Publication

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

smURFP-P2A-GFP

Use

AAV

Available since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_EF1a-FLEx(FRT)-GCaMP8m

Plasmid

#252530

Purpose

Expresses the genetically encoded calcium indicator GCaMP8m in Flp-expressing cells under the EF1a promoter for calcium imaging.

Depositor

Gordon Fishell

Publication

Bhatti Mazo et al Nature. 2026 Aug;656(8127):415-424. doi:

Insert

GCaMP8m

Use

AAV

Expression

Mammalian

Available since

May 27, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_g5-HT2h

Plasmid

#208721

Purpose

Expresses the green 5-HT sensor GRAB_g5-HT2h in neurons

Depositor

Yulong Li

Publication

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Insert

Green fluorescent 5-HT sensor GRAB_g5-HT2h

Use

AAV

Promoter

hSyn

Available since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV Syn CaMPARI2-F391W_L398V-human-a-Tubulin (high affinity TubuTag)

Plasmid

#164193

Purpose

Freeze-frame imaging of dendritic calcium signals High calcium affinity (CaMPARI2_F391W_L398V moiety Kd=174nM)

Depositor

Thomas Oertner

Publication

Perez-Alvarez et al Front Mol Neurosci. 2021 Mar 4;14:635820

Inserts

CamPARI2
Human α Tubulin

Use

AAV

Tags

6 His tag and NES

Expression

Mammalian

Mutation

F391W-L398V

Promoter

Syn

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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AiP12211 - pAAV-AiE0050m_3xC2-minCMV-SYFP2-WPRE3-BGHpA (Alias: CN2211)

Plasmid

#223974

Purpose

AiE0050m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L4/5_IT neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

minBG

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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AiP13997 - pAAV-AiE0410m-minBG-iCre(R297T)-BGHpA (Alias: CN3997)

Plasmid

#230388

Purpose

AiE0410m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre(R297T)

Use

AAV

Promoter

minBG

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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AiP13786 - pAAV-AiE0140h-minBG-iCre(R297T)-BGHpA (Alias: CN3786)

Plasmid

#230382

Purpose

AiE0140h is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre(R297T)

Use

AAV

Promoter

minRho

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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AiP12360 - pAAV-AiE0519h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2360)

Plasmid

#223986

Purpose

AiE0519h is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Sst+ neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

minBG

Available since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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AiP13568 - pAAV-AiE0387m_3xC1-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3568)

Plasmid

#208142

Purpose

AiE0387m_3xC1 is an optimized enhancer sequence designed to drive AAV-mediated transgene expression in brain astrocytes

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

Beta Globin minimal promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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AiP13098 - pAAV-AiE0380h-minBglobin-SYFP2-WPRE3-BGHpA (Alias: CN3098)

Plasmid

#208123

Purpose

Direct-expressing SYFP2 in astrocytes AAV Virus

Depositor

Allen Institute for Brain Science, Boaz Levi

Publication

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

Beta Globin minimal promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-S5E8-dTom-nlsdTom

Plasmid

#135643

Purpose

AAV vector to drive the expression of dTomato in PV cortical interneuronsunder the control of the E8 regulatory element

Depositor

Jordane Dimidschstein

Publication

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Insert

dTom-nlsdTom

Use

AAV

Mutation

None

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pRC_RR_VP2/3

Plasmid

#65725

Purpose

wt VP2/VP3 only to participate in capsid assembly; no ITR's

Depositor

Junghae Suh

Publication

Judd et al Mol Ther Nucleic Acids. 2012 Nov 13;1:e5

Insert

empty

Available since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAC153-dual-dCas9VP96-sgExpression

Plasmid

#48239

Purpose

Dual expression construct expressing both dCas9VP96 and sgRNA from separate promoters

Depositor

Rudolf Jaenisch

Publication

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas9

Use

CRISPR

Tags

HA-Tag, HA-tag, and VP96

Expression

Mammalian

Mutation

D10A H840A

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn1-DIO-VGLUT2:miniSOG

Plasmid

#214200

Purpose

For Cre-dependent expression of VGLUT2:miniSOG (miniSOG is lumen-facing and HA-tagged)

Depositor

Thomas Hnasko

Publication

Steinkellner et al ACS Chem Neurosci. 2021 Feb 17;12(4):626

Insert

Slc17a6 tagged with miniSOG and hemagglutinin

Use

AAV and Cre/Lox

Tags

miniSOG (tagged with HA) is fused to the first in…

Expression

Mammalian

Available since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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