We narrowed to 66,707 results for: nin
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Plasmid#117236Purposencas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickaseDepositorInsertncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Hs.KRAS4b G12V
Plasmid#83132PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12V mutationDepositorAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONR223-AKT1
Plasmid#23752DepositorInsertAKT1 (AKT1 Human)
UseGateway CloningAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TRIPZ-bioGEF-UBwt-PURO
Plasmid#208045PurposeEnables inducible expression of low-affinity Avitag-Ubwt; to perform bioE3; selection with puromycinDepositorAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Flag-GFP-HRP-TM_pLX304
Plasmid#202021Purposeexpresses GFP-HRP anchored to the extracellular side of the plasma membrane, lentiviral vectorDepositorInsertFlag-GFP-HRP-TM
UseLentiviralTagsFlag and GFPExpressionMammalianPromoterCMVAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
OaAEP1_C247A
Plasmid#200307PurposeBacterial expression plasmids for production of C247A mutant of asparaginyl endopeptidase 1 from O. affinis (OaAEP1_C247A)DepositorInsertAsparaginyl endopeptidase from Oldenlandia affinis
TagsSmt3ExpressionBacterialMutationCysteine 247 to AlaninePromoterT7Available sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSL1145 (pSPIN, pBBR1 backbone, R*MmeI)
Plasmid#160736PurposeSingle-plasmid V. cholerae CAST, encodes all proteins, crRNA, and donor DNA. Non-targeting crRNA with BsaI sites for spacer cloning. Mini-tn has MmeI site in R end for Tn-seq. pBBR1 backbone.DepositorInsertVchCAST proteins, crRNA, and donor DNA
ExpressionBacterialPromoterJ23119Available sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDFDuet-nsp7-nsp8
Plasmid#159092PurposeCoexpression construct of Nsp7 with an N-terminal His-tag and Nsp8DepositorInsertsNSP7
NSP8
TagsHisExpressionBacterialPromoterT7Available sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST
Plasmid#109048PurposeAnion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal dendrites under the control of internal ribosome entry sequence in a viral vectorDepositorHas serviceAAV9InserteGtACR1-ST
UseAAVPromoterCAGAvailable sinceJuly 11, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDECKO-mCherry GFP
Plasmid#78535PurposeControl plasmid (paired gRNAs against GFP)DepositorInsertgRNA1+scaffold+H1promoter+gRNA2
UseLentiviralExpressionMammalianPromoterU6 (for expressing sgRNA) and U6 promoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TOPOII p48 (DM#261)
Plasmid#15026DepositorInsertp48 (Ptf1a Mouse)
ExpressionBacterialAvailable sinceOct. 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Sox15
Plasmid#13359DepositorAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
35S-GAL4BD-GWY/pAM
Plasmid#201233PurposeUsed to produce and express in planta N-terminal fusion proteins with the GAL4 BDDepositorInsert35S-GAL4BD-GWY
ExpressionPlantPromoterCaMV 35SAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pN251
Plasmid#188471PurposeLentiviral construct encoding doxycycline-inducible enCas12a-HF (via TetON3G)DepositorInsertenCas12a-HF (gift from Dr. Keith Joung)
UseLentiviralExpressionBacterial and MammalianAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKM7
Plasmid#154164PurposemCherry expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.DepositorInsertmCherry
ExpressionInsectPromoterIE1 promoter and hr5 enhancer from Autographa cal…Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MH6-RspWYL1
Plasmid#108306PurposeExpresses E. coli codon optimized RspWYL1 in the pET28a backbone.DepositorInsertRspWYL1
UseCRISPRTagsmH6 tagExpressionBacterialPromoterlacAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-N-3XFLAG-Gsk3b
Plasmid#123592DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertglycogen synthase kinase 3 beta (Gsk3b Mouse)
TagsFLAGExpressionMammalianAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP/KATNA1
Plasmid#89316PurposeExpression of rat GFP/Katanin A subunit in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertKATNA1 (Katna1 Rat)
TagsGFPExpressionMammalianPromoterCMVAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-PIK4CB
Plasmid#23839DepositorAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-T7-ABE8.20m-nSpRY-P2A-EGFP (KAC1335)
Plasmid#185917PurposepCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman codon optimized ABE8.20m-nSpRY-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…PromoterCMV and T7Available sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only