We narrowed to 5,913 results for: pcDNA 3.1

1. REEP5-mEmerald

   Plasmid

   #186964

   Purpose

   Express REEP5/DP1 with C-terminus mEmerald

   Depositor

   Ke Xu

   Article

   Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

   Insert

   Reep5 (Reep5 Mouse)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CMV Promoter

   Available since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTS2395-Tier1-PhCMV-Igk_mRuby2_KDEL

   Plasmid

   #169533

   Purpose

   Tier-1 vector encoding PhCMV-driven red fluorescent protein mRuby2 localized to the ER (PhCMV-Igk-mRuby2-KDEL-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven ER-localized monomeric red fluorescent protein mRuby2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Luc-noPS

   Plasmid

   #177940

   Purpose

   Transient mammalian expression of Luc2 (firefly luciferase). Lacks packaging sequence and is used as a negative control transfer plasmid for SARS-CoV-2 virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   Luc2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. hGATA-1 WT

   Plasmid

   #118352

   Purpose

   This plasmid expresses human GATA-1 WT.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

   Insert

   hGATA-1 (GATA1 Human)
   

   Use

   Tags

   myc and 6xHIS

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. UKNP1.7.1

   Plasmid

   #97371

   Purpose

   Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

   Depositor

   Jonathan Ball

   Article

   Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

   Insert

   HCV E1E2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSL3402 (pTnsABf_Pse)

   Plasmid

   #200901

   Purpose

   Expresses human codon-optimized Pseudoalteromonas TnsA-TnsB fusion from a CMV promoter.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   PseTnsA-TnsB
   

   Use

   Tags

   Internal NLS between TnsA-TnsB fusion

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Opto-alpha1AR

   Plasmid

   #106064

   Purpose

   Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-alpha1AR; Opto-alpha1AR; F6; 717)

   Depositor

   Harald Janovjak

   Article

   Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

   Insert

   Opto-alpha1AR
   

   Use

   Tags

   Rhodopsin-1D4 and VSV-G

   Expression

   Mammalian

   Mutation

   Chimeric receptor protein

   Promoter

   CMV

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. NSP3-FR-2Strep

   Plasmid

   #210315

   Purpose

   Expresses SARS-CoV-2 NSP3 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   NSP3 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   2Strep

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDN34

   Plasmid

   #61343

   Purpose

   mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/PKIt NES)

   Depositor

   Roland Eils, Barbara Di Ventura

   Article

   Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

   Insert

   mCherry
   

   Use

   Tags

   GS linker-AsLov2-biNLS2 and PKIt NES

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. SPLICS Mt-ER- PM Short P2A

    Plasmid

    #164110

    Purpose

    Detect the short Plasma membrane-mitochondria-Endoplasmic reticulum contact

    Depositor

    Marisa Brini, Tito Calì

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split GFP and YFP PM- Mt-ER Short P2A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. FlagETR3 (CELF2)

    Plasmid

    #96900

    Purpose

    expression of Flag-tagged human CELF2 (aka CUGBP2)

    Depositor

    Thomas Cooper

    Article

    Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

    Insert

    human CELF2 cDNA (CELF2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. Opto-D2R

    Plasmid

    #106068

    Purpose

    Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-D2R; Opto-D2R; F10; 721)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-D2R
    

    Use

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. Opto-GPR139

    Plasmid

    #106044

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR139; Opto-GPR139; D10)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR139
    

    Use

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. human-GFAP-EGFP

    Plasmid

    #169473

    Purpose

    We generated a human-GFAP-EGFP fusion construct by randomly inserting the coding region of EFGP into the coding region of hGFAP.

    Depositor

    Federico Herrera

    Article

    Letra-Vilela et al Experimental Results (2020), Volume 1, e

    Insert

    EGFP-hGFAP (GFAP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. YFP Gamma 8

    Plasmid

    #36106

    Depositor

    Narasimhan Gautam

    Article

    Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

    Insert

    G protein subunit Gamma 8 (GNG8 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 3, 2012

    Availability

    Academic Institutions and Nonprofits only

16. Opto-M2R

    Plasmid

    #106070

    Purpose

    Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-M2R; Opto-M2R; F12; 723)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-M2R
    

    Use

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. CMV-SpyCas9

    Plasmid

    #113033

    Purpose

    SpyCas9 expression in mammalian cells (CMV promoter driven)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    SpyCas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP-CBS (WT)

    Plasmid

    #182923

    Purpose

    Mammalian expression plasmid for human CBS

    Depositor

    Philip Eaton

    Article

    Switzer et al Proc Natl Acad Sci U S A. 2023 Jan 17;12

    Insert

    Cystathionine Beta-Synthase (CBS Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. Opto-M3R

    Plasmid

    #106071

    Purpose

    Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-M3R; Opto-M3R; H1; 724)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-M3R
    

    Use

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pY026

    Plasmid

    #84741

    Purpose

    Expresses huAsCpf1 and crRNA guide

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Insert

    huAsCpf1
    

    Use

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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