We narrowed to 12,703 results for: sequence

1. O-IL8-15

   Plasmid

   #166544

   Purpose

   scFv of a human scaffold targeting Interleukin-8. Antigen coverage aa 28-99 of 99, full-length excl. signal peptide

   Depositor

   Susanne Gräslund

   Insert

   single chain-Fv, scFv
   

   Use

   Recombinant antibody expression

   Tags

   3xFLAG, Sortag, His6 and OmpA leader sequence

   Expression

   Bacterial

   Mutation

   Promoter

   LacZ

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHBS985 TDP43 RRM-GFP G348C

   Plasmid

   #107832

   Purpose

   To test the effect of sequence on TDP43 droplet properties

   Depositor

   Rajat Rohatgi

   Insert

   TARDBP mutant (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G348C mutation in LLPS reporter

   Promoter

   Available since

   April 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKG212

   Plasmid

   #69758

   Purpose

   Retroviral expression of CENP-T aa 1-242

   Depositor

   Iain Cheeseman

   Insert

   CENP-T aa 1-242 (CENPT Human)
   

   Use

   Retroviral

   Tags

   LacI-NLS and mCherry

   Expression

   Mammalian

   Mutation

   e.coli optimized sequence

   Promoter

   Available since

   Nov. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pHBS996 TDP43 RRM-GFP A382T

   Plasmid

   #107816

   Purpose

   To test the effect of sequence on TDP43 droplet properties

   Depositor

   Rajat Rohatgi

   Insert

   TARDBP mutant (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A382T mutation in LLPS reporter

   Promoter

   Available since

   April 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHBS1051 TDP43 RRM-GFP F-Y

   Plasmid

   #107802

   Purpose

   To test the effect of sequence on TDP43 droplet properties

   Depositor

   Rajat Rohatgi

   Insert

   TARDBP mutant (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   All phenylalanine to tyrosine in LLPS reporter

   Promoter

   Available since

   April 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHBS961 TDP43 RRM-GFP G295S

   Plasmid

   #107820

   Purpose

   To test the effect of sequence on TDP43 droplet properties

   Depositor

   Rajat Rohatgi

   Insert

   TARDBP mutant (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G295S mutation in LLPS reporter

   Promoter

   Available since

   April 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHBS1330 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 WT]

   Plasmid

   #107839

   Purpose

   To test the effect of sequence on TDP43 splicing activity

   Depositor

   Rajat Rohatgi

   Insert

   TARDBP (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Wildtype in splicing reporter

   Promoter

   Available since

   April 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. p203 Dync1li1 N235Y

   Plasmid

   #26451

   Depositor

   Elizabeth Fisher

   Insert

   cytoplasmic dynein light intermediate chain 1 N235Y (Dync1li1 Mouse)
   

   Use

   Subcloning and sequencing

   Tags

   Expression

   Mutation

   changed Asparagine 235 to Tyrosine

   Promoter

   Available since

   Dec. 2, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pHBS932 TDP43 RRM-GFP Φ-S

   Plasmid

   #107798

   Purpose

   To test the effect of sequence on TDP43 droplet properties

   Depositor

   Rajat Rohatgi

   Insert

   TARDBP mutant (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   All hydrophobics to serine

   Promoter

   Available since

   April 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. Sc Pol3-FLAG(Ec)-pRSF405/GAL

    Plasmid

    #239198

    Purpose

    Overexpress Sc Pol3(C-terminal 3X FLAG tag) when integrated into yeast (S. cer)

    Depositor

    Michael O'Donnell

    Insert

    Pol3 (POL3 Budding Yeast)
    

    Use

    Tags

    3X FLAG

    Expression

    Yeast

    Mutation

    E. coli codon bias (synthetic gene), but same ami…

    Promoter

    Available since

    June 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. OgeuIscB CTG RNA

    Plasmid

    #222865

    Purpose

    This plasmid codes for the guide of the OgeuIscB with a CTG target

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Insert

    OgeuIscB omega RNA with a (CTG)6 target sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available since

    Oct. 17, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. OgeuIscB GCT RNA

    Plasmid

    #222862

    Purpose

    This plasmid codes for the guide of the OgeuIscB with a GCT target

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Insert

    OgeuIscB omega RNA with a (GCT)6 target sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available since

    Oct. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. OgeuIscB GCA RNA

    Plasmid

    #222864

    Purpose

    This plasmid codes for the guide of the OgeuIscB with a GCA target

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Insert

    OgeuIscB omega RNA with a (GCA)6 target sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available since

    Oct. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. OgeuIscB TGC RNA

    Plasmid

    #222863

    Purpose

    This plasmid codes for the guide of the OgeuIscB with a TGC target

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Insert

    OgeuIscB omega RNA with a (TGC)6 target sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available since

    Oct. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. OgeuIscB AGC RNA

    Plasmid

    #222866

    Purpose

    This plasmid codes for the guide of the OgeuIscB with a AGC target

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Insert

    OgeuIscB omega RNA with a (AGC)6 target sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available since

    Oct. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. OgeuIscB CAG RNA

    Plasmid

    #222867

    Purpose

    This plasmid codes for the guide of the OgeuIscB with a CAG target

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Insert

    OgeuIscB omega RNA with a (CAG)6 target sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available since

    Oct. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. PCDF Bravo-PotH-OL3/2sub

    Plasmid

    #216752

    Purpose

    Study the interaction of PotH in E. coli, specifically focusing on the variant in which outer loop 2 (OL2) is substituted with OL3 in its interaction with exogenous peptides.

    Depositor

    Gordon Laurie

    Insert

    potH (potH E. coli (Bacteria))
    

    Use

    Tags

    10x His

    Expression

    Bacterial

    Mutation

    Outer loop 2 (OL2) with the sequence of [WMGILKNN…

    Promoter

    Available since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. MUT_S1-H1,NR

    Plasmid

    #218395

    Purpose

    3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, both randomly mutated to avoid sequence repeats, and AG>GG mutation in final 3' splice site

    Depositor

    Oded Regev

    Insert

    Modified CHO DHFR minigene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetracycline-responsive promoter

    Available since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAS_MmaPylT_EF1_NES-MmaPylRS WT

    Plasmid

    #174901

    Purpose

    expression of Mma PylRS with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integration

    Depositor

    Simon Elsaesser

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Use

    Tags

    FLAG and NES

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pnYC-NpM-HsNot10_25-707_AF

    Plasmid

    #148678

    Purpose

    Bacterial Expression of HsNot10_25-707

    Depositor

    Elisa Izaurralde

    Insert

    HsNot10_25-707 (CNOT10 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    one silent mutation compared to the sequence give…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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