We narrowed to 14,669 results for: Egf
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Plasmid#223117PurposepCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MRKCRS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFPDepositorInserthuman codon optimized TadA8e-SpCas9(MRKCRS)-P2A-EGFP
UseCRISPRTagsBPNLS-P2A-EGFP and BPNLS-TadA8eExpressionMammalianMutationTadA8e mutations; nSpCas9(D10A/MRKCRS)=D1135M, S1…PromoterCMV and T7Available SinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynI-EGFP-WPRE-hGH poly(A) (Alex_02)
Plasmid#71651PurposeAAV expression of EGFP in human and mouse neuronsDepositorInsertEGFP
UseAAVExpressionMammalianAvailable SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
KL158 - pBlueScript II SK-T2A-eGFP-SV40pA Podxl donor
Plasmid#232936PurposeDonor plasmid for knock-in of T2A-eGFP-SV40pA at the mouse Podxl locus. Used with PX459-sgRNA047_Podxl sgRNA.DepositorInsertT2A-eGFP-SV40pA with Podxl homology arms
UseMouse TargetingAvailable SinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1_GST-EGFP-ATG13_IDR_191-517aa_(M196-H214 11xA)
Plasmid#223779PurposeExpression of recombinant protein for purificationDepositorInsertATG13 IDR (ATG13 Human)
TagsGST-EGFPExpressionBacterialMutationexpresses aa 191-517, with M196A S197A R199A G202…Available SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1_GST-EGFP-ATG13_IDR_delta206-230 (191-205_231-517aa)
Plasmid#223787PurposeExpression of recombinant protein for purificationDepositorInsertATG13 IDR (ATG13 Human)
TagsGST-EGFPExpressionBacterialMutationexpresses aa 191-517, with 206-230 deletedAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FKBP-mEGFP-WIPI2 IDR (364-425aa)
Plasmid#223758PurposeStable expression and inducible recruitment of protein in mammalian cell cultureDepositorAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_6xHis-TEV-EGFP-FIP200-CTR(1429-1591aa)
Plasmid#223724PurposeExpression of recombinant protein for purificationDepositorInsertFIP200-CTR (RB1CC1 Human)
Tags6xHis-TEV-EGFPExpressionBacterialMutationaa 1429-1591 onlyAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV.U6-sasgRNA(SapI)_Ple155-CI-EGFP-W3SL_BbsI(GGA)
Plasmid#231367PurposePle155-driven EGFP, also encoding U6-driven sgRNA cassette, with SapI sites to clone crRNA sequenceDepositorInsertEGFP
UseAAVAvailable SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
Const.3 EGFP-P2A-dTomato-T2A-TagBFP-PS9
Plasmid#231907PurposeFor packaging EGFP, dTomato and TagBFP mRNA into SARS-CoV-2 virus-like particles (VLPs)DepositorInsertEGFP-P2A-dTomato-T2A-TagBFP
ExpressionMammalianPromoterCMVAvailable SinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
Const.5 EGFP-P2A-dTomato-IRES-Luc-PS9
Plasmid#231909PurposeFor packaging EGFP, dTomato and firefly luciferase mRNA into SARS-CoV-2 virus-like particles (VLPs)DepositorInsertEGFP-P2A-dTomato-IRES-Luc
ExpressionMammalianPromoterCMVAvailable SinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME nls EGFP WPRE bGH pA (JDW 488)
Plasmid#224540PurposeA Gateway compatible middle entry clone containing a 3x-NLS-EGFP for labeling the nucleusDepositorInsertnls-EGFP-WPRE-bGH-pA
UseGateway cloningAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO-puro SNAP-CD86-eGFP-FKBP(f36v)
Plasmid#200283PurposeMembrane protein fusion with extracellular SNAPtag and intracellular FKBP(f36v) dimerization domain and GFP reporter under Tetracycline-inducible promoterDepositorInsertSNAPtag-eGFP-CD86tm-eGFP-FKBP(f36v)
UseLentiviralAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX552-EF1a-DIO Chronos-eGFP(with gRNA scaffold)
Plasmid#199582PurposeExpresses Chronos-eGFP in a Cre-dependent mannerDepositorInsertChronos eGFP
UseAAV, CRISPR, and Cre/LoxTagsGFPExpressionMammalianPromoterEF1a intron AAvailable SinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1_LIR1-35S-eGFP-T35S-SIR-LIR2 (GB4312)
Plasmid#225435PurposeBeYDV LIR1 + TU 35S:eGFP:T35S + BeYDV SIR-LIR2.DepositorInsertLIR1:35S:eGFP:T35S-SIR:LIR2
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHIV-NAT-T2A-hCD52-eGFP-NFATC2IP-SPR
Plasmid#221447PurposeLentiviral mammalian expression vector of eGFP-tagged NFATC2IP ORF that is fused with S-phase restricted (SPR) degron at its C-terminusDepositorInsertNFATC2IP (NFATC2IP Human)
UseLentiviralTagseGFPExpressionMammalianMutationSPR degron: hGEM (1-110) fragment that is fused t…PromoterCMVAvailable SinceJuly 3, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOTTC1642 - pAAV EF1a EGFP-KASH with mTACR1 gRNAs
Plasmid#195018PurposeAn adeno-associated viral vector expressing eGFP-KASH and two guides targeting mouse TACR1DepositorInsertsCCGTATAGGCGGCTGCCCAA
EGFP-KASH
TTCCGTGGTGGGCAACGTAG
UseAAVTagsKASH domain of Nesprin2PromoterEF1a, hU6, and mU6Available SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNES-SET-EMD-EGFP (C-NES-SET-EMD)
Plasmid#217770PurposeFluorescent reporter for ceramide (putative, mammalian expression)DepositorInsertSET (SET Human)
TagsEGFPExpressionMammalianMutationEMD domain (aa 70-226) plus nuclear export sequen…PromoterCMVAvailable SinceJune 12, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNES-PRKCZ-C20-EGFP (C-NES-PRKCZ-C20)
Plasmid#217763PurposeFluorescent reporter for ceramide (putative, mammalian expression)DepositorInsertProtein kinase C zeta (PRKCZ Human)
TagsEGFPExpressionMammalianMutationNES (ALQKKLEELELDEAPVAT) plus C20 domain (aa 405-…PromoterCMVAvailable SinceJune 11, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
lenti-EGFR-L858R-T790M-dual-nick sgRNA
Plasmid#214101PurposeLentiviral vector expressing nicking sgRNAs for the induction of EGFR L858R and T790M mutations, through tandem U6 expression of the two sgRNAs using independent U6 promoters.DepositorInsertEGFR L858R nicking sgRNA/EGFR T790M nicking sgRNA
UseLentiviralExpressionMammalianPromoterhU6Available SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGS58_6xHis-EGFP-CENP-T[1-242/3D (T11, T27, T85)] - I3-01
Plasmid#211890PurposeBacterial expression of CENP-T[1-242/3D]-I3-01DepositorInsertCENP-T[1-242/3D]-I3-01 (CENPT Synthetic, Human)
ExpressionBacterialMutationT11D, T27D, T85DAvailable SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV UBC OSBP(PH)-APEX2-3xFLAG-IRES-eGFP
Plasmid#218637PurposeProximity biotinylation control of OSBP(PH domain)DepositorInsertOSBP (OSBP Human)
UseLentiviralTagsAPEX2-3xFLAGExpressionMammalianMutationPH domain onlyPromoterUBCAvailable SinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CMV-Nier1s-3NLS-P2A-EGFP-3NLS
Plasmid#217478PurposeMammalian expression plasmid for far-red fluorescent Ca2+ indicator Nier1sDepositorInsertNier1s
Tags3xNLS, P2A-EGFP-3xNLSExpressionMammalianPromoterCMVAvailable SinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CMV-Nier1b-3NLS-P2A-EGFP-3NLS
Plasmid#217479PurposeMammalian expression plasmid for far-red fluorescent Ca2+ indicator Nier1bDepositorInsertNier1b
Tags3xNLS, P2A-EGFP-3xNLSExpressionMammalianPromoterCMVAvailable SinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTY186 EGFP-T2A-ALFA-ORF6 SARS-CoV-2
Plasmid#204976PurposeCo-express EGFP and SARS-CoV-2 ORF6 N-terminally labeled with ALFA tagDepositorAvailable SinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGS275_6xHis-EGFP-CENP-T[1-242/3D (T11, T27, T85)]
Plasmid#211891PurposeBacterial expression of CENP-T[1-242/3D]DepositorAvailable SinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-EGFR*-CUbo(K48R K63R)-GFP
Plasmid#212818PurposeConstitutive or doxycycline-inducible expression of EGFR*-CUbo(K48R K63R)-GFP in mammalian cellsDepositorAvailable SinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-191~307-mEGFP(A206K)-TwStrep
Plasmid#208626Purposefor E. coli expression of truncated human RPL3 (ENST00000216146), aa191~307, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 191~307 (RPL3 Human)
Tags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 191~307, other residues not includedPromoterT7Available SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-299~398-mEGFP(A206K)-TwStrep
Plasmid#208627Purposefor E. coli expression of truncated human RPL3 (ENST00000216146), aa299~398, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 299~398 (RPL3 Human)
Tags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 299~398, other residues not includedPromoterT7Available SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-212~285-mEGFP(A206K)-TwStrep
Plasmid#208629Purposefor E. coli expression of truncated human RPL3 (ENST00000216146), aa212~285, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 212~285 (RPL3 Human)
Tags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 212~285, other residues not includedPromoterT7Available SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFH2.99_PB-EPN1-MCP_I-T2A-eGFP-PGK-Blast
Plasmid#205544PurposeExpress EPN1-MCP-I-T2A-eGFP and blasticidin resistance markerDepositorInsertEPN1-MCP-I
UsePiggybacExpressionMammalianMutationWTPromoterEF1Available SinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only