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lentiCRISPRv2_sgCtrl#2

Plasmid

#174149

Purpose

Lentiviral vector expressing Cas9 and a control sgRNA that targets a safe harbor site

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

Control sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pKM342

Plasmid

#71486

Purpose

Contains loxP-hyg-loxP cassette for recombineering; HygR, AmpR.

Depositor

Kenan Murphy

Article

Murphy et al Methods Mol Biol. 2015;1285:177-99. doi:

Insert

loxP-hyg-loxP cassette

Use

Source of loxp-hyg-loxp for pcr amplification.

Expression

Bacterial

Available Since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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pPSP6+t

Plasmid

#48149

Purpose

Shuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage SP6

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-LSL-mCherry-shLuci

Plasmid

#178590

Purpose

Cre-dependent expression of mCherry and a shRNA against luciferase under the constitutive promoter

Depositor

Chun-Li Zhang

Article

Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

Insert

miRE-Luciferase

Use

AAV

Expression

Mammalian

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pRSAN-QUE37C

Plasmid

#129312

Purpose

ATP measurement

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN-37C

Expression

Bacterial

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pSEVA221T7RNAP

Plasmid

#167974

Purpose

Expresses T7RNAP in E. coli

Depositor

Luis Ángel Fernández

Article

Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

Insert

T7RNAP (T7p07 Synthetic)

Promoter

TetR-Ptet

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pBTSG

Plasmid

#159420

Purpose

Transient expression of proteins with a C-terminal thermostable GFP (TGP) tag in mammalian cells

Depositor

Dianfan Li

Article

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

thermostable GFP (TGP)

Tags

polyhistidine (poly-His)

Expression

Mammalian

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pETSG

Plasmid

#159418

Purpose

Expression of proteins with a C-terminal thermostable GFP (TGP) tag in Escherichia coli

Depositor

Dianfan Li

Article

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

thermostable GFP (TGP)

Tags

polyhistidine (poly-His)

Expression

Bacterial

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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JB93

Plasmid

#128904

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

Rab7AQL-BirA*-HA-MAO (RAB7A Human)

Expression

Mammalian

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCF221

Plasmid

#121669

Purpose

U6-sgRNA EF1a-mCherry

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Insert

mCherry

Use

Lentiviral

Promoter

U6

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pQLinkHD-SYNZIP6

Plasmid

#80662

Purpose

Encodes the synthetic coiled coil peptide SYNZIP6

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP6

Tags

6xHis

Expression

Bacterial

Available Since

March 4, 2019

Availability

Academic Institutions and Nonprofits only

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pQLinkHD-SYNZIP5

Plasmid

#80661

Purpose

Encodes the synthetic coiled coil peptide SYNZIP5

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP5

Tags

6xHis

Expression

Bacterial

Available Since

March 4, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-SYNZIP17

Plasmid

#80671

Purpose

Encodes the synthetic coiled coil peptide SYNZIP17

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP17

Use

Gateway Cloning

Available Since

Nov. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pBSV2G_PflaB-mEGFPBb

Plasmid

#118234

Purpose

Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven mEGFP

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

flagellin promoter driven mEGFP

Expression

Bacterial

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pBSV2G_PflaB-msfYFPBb

Plasmid

#118233

Purpose

Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven msfYFP

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

flagellin promoter driven msfYFP

Expression

Bacterial

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pQLinkHD-SYNZIP4

Plasmid

#80660

Purpose

Encodes the synthetic coiled coil peptide SYNZIP4

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP4

Tags

6xHis

Expression

Bacterial

Available Since

Feb. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pMB72

Plasmid

#73088

Purpose

C-terminal GFP-2xTEV-Avi vector, for fosmid recombineering

Depositor

Mike Boxem

Article

Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

Insert

GFP-Avi tag

Tags

GFP and Avi

Expression

Worm

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-MAVS-M142A(KaganF61)

Plasmid

#51999

Purpose

Expresses the human MAVS CDS containing the point mutation M142A and an N-terminal HA tag "strong translational context"

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS

Tags

HA

Expression

Mammalian

Mutation

Met 142 to Ala

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
pShuttle-FEN1mWT

Plasmid

#35032

Depositor

Sheila Stewart

Article

Saharia et al J Biol Chem. 2010 Aug 27;285(35):27057-6

Insert

FEN1mWT

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only
pGS775

Plasmid

#20169

Depositor

Stephen West

Article

Dunderdale et al J Biol Chem. 1994 Feb 18. 269(7):5187-94

Insert

RuvC

Expression

Bacterial

Mutation

none

Available Since

April 1, 2009

Availability

Academic Institutions and Nonprofits only