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We narrowed to 9,400 results for: Coli

Showing: 7381 - 7410 of 9400 results

  2. pHS2096

    Plasmid
    #239835
    Purpose
    NovaIscB E. coli expression for recombinant purification
    Depositor
    Feng Zhang
    Article
    Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038
    Insert
    NovaIscB
    Use
    Tags
    14xHis-TwinStrep-bdSUMO
    Expression
    Bacterial
    Mutation
    Insertion of REC domain from NbaCas9-1 and swap o…
    Promoter
    T7
    Available Since
    July 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    239835-plasmid-map-sequence-id-479109

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  3. B8-pVTCB052

    Plasmid
    #231302
    Purpose
    Plasmid used for expression of AI-designed GFP protein B8 in E. coli
    Depositor
    Alexander Rives
    Insert
    B8
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    March 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    231302-plasmid-map-sequence-id-463621

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  4. pdnCas3-CDA1

    Plasmid
    #229535
    Purpose
    pMV_hyg encoding dnCas3(H74A+D75A)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning
    Depositor
    Kevin Verstrepen
    Article
    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:
    Inserts
    dnCas3
    Cytidine deaminase
    Uracil glycosylase inhibitor
    Use
    Tags
    XTEN
    Expression
    Bacterial and Yeast
    Mutation
    codon optimized for S.cerevisiae and codon optimi…
    Promoter
    Available Since
    Jan. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    229535-plasmid-map-sequence-id-459013

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  5. pdnCas3-APOBEC1

    Plasmid
    #229537
    Purpose
    pMV_hyg encoding dnCas3(H74A+D75A)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning
    Depositor
    Kevin Verstrepen
    Article
    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:
    Inserts
    rAPOBEC1
    dnCas3
    Uracil glycosylase inhibitor
    Use
    Tags
    XTEN
    Expression
    Bacterial and Yeast
    Mutation
    codon optimized for S.cerevisiae and codon optimi…
    Promoter
    Available Since
    Jan. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    229537-plasmid-map-sequence-id-459005

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  8. pET28a-Fgß-Coh7-ddFLN4-ELP-HIS-ybbR

    Plasmid
    #169135
    Purpose
    E. coli expression of Coh7 domain containing Fgß tag, ybbR tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.
    Depositor
    Michael Nash
    Article
    Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi
    Insert
    Coh7
    Use
    Tags
    3xELP linker, 6x Histag, Fgβ peptide, ddFLN4 tag,…
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Oct. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    169135-plasmid-map-sequence-id-361163

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  12. pC3.1_CMV_oROS-G_LF(C199S)

    Plasmid
    #216112
    Purpose
    Expresses the loss-of-function mutations C199S of the genetically encoded green fluorescent hydrogen peroxide sensor oROS-G in mamalian cells.
    Depositor
    Andre Berndt
    Article
    Lee et al bioRxiv 2024.01.31.578117
    Insert
    oROS-G_LF(C199S)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    216112-plasmid-map-sequence-id-434850

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  18. pCgVchCAST-crtYf

    Plasmid
    #203816
    Purpose
    Expression of CRISPR-associated transposases, shuttle vector for Corynebacterium glutamicum / E. coli
    Depositor
    Sheng Yang
    Article
    Yang et al ACS Synth Biol. 2023 Jul 21;12(7):2198-2
    Insert
    VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(crtYf)
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    203816-plasmid-map-sequence-id-403367

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  22. pXD70DAmt1-DadR/DadS-Pdadh-DadhABC(R506S, A2)

    Plasmid
    #191631
    Purpose
    E. coli - Eggerthella lenta shuttle plasmid (KanR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain (R506S mutation) with transcriptional regulators DadR/DadS
    Depositor
    Emily Balskus
    Article
    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:
    Insert
    Dopamine dehydroxylase (R506S mutation, inactive form)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    191631-plasmid-map-sequence-id-375966

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  23. pXD71Cas10.0RTcgr-Pct5.1-crRNA(AarI)-RT(Δcgr12)

    Plasmid
    #191651
    Purpose
    E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(nontargeting spacer) cgr1/cgr2-deleting repair template, used as negative control for pXD71Cas10.4RT4
    Depositor
    Emily Balskus
    Article
    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:
    Insert
    CymR
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    191651-plasmid-map-sequence-id-375980

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  24. KTK_113

    Plasmid
    #180540
    Purpose
    Entry vector containing Signal peptide region of CsgA including SecB-N22 sequenced
    Depositor
    Tom Ellis
    Article
    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-
    Insert
    Signal peptide region of CsgA including SecB-N22 sequenced
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    180540-plasmid-map-sequence-id-369685

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  25. pBICI_high ori

    Plasmid
    #179273
    Purpose
    Expresses CuCBP and BaSP in E. coli BL21(DE3)
    Depositor
    Bernd Nidetzky
    Article
    Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d
    Inserts
    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    Use
    Synthetic Biology
    Tags
    His Tag and Strep - Tag II
    Expression
    Bacterial
    Mutation
    Promoter
    T7 lacO
    Available Since
    April 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    179273-plasmid-map-sequence-id-358714

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  26. pBtPIPLC_N168C His

    Plasmid
    #173809
    Purpose
    Expression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cells
    Depositor
    Mary F Roberts, Anne Gershenson
    Article
    He et al J Biol Chem. 2015 Jul 31;290(31):19334-4
    Insert
    Bacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
    Use
    Tags
    6XHis
    Expression
    Bacterial
    Mutation
    Asn168Cys for labeling
    Promoter
    T7
    Available Since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    173809-plasmid-map-sequence-id-341161

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  30. pET28a-ybbr-XMod-Doc (BMA-KO, S199AzF)-HIS

    Plasmid
    #153444
    Purpose
    E. coli expression (amber suppression) of Rc. XDocB binding mode A knock-out mutant with serine at position 199 replaced with amber codon.
    Depositor
    Michael Nash
    Article
    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:
    Insert
    Rc.XDocB
    Use
    Tags
    6xHis and ybbr tag
    Expression
    Bacterial
    Mutation
    Serine 199 was mutated to amber codon (TAG). Argi…
    Promoter
    T7 promoter
    Available Since
    July 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    153444-plasmid-map-sequence-id-306456

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Showing: 7381 - 7410 of 9400 results