We narrowed to 25,531 results for: Nov;
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Plasmid#86870PurposeTo express SF3B6 with a GFP and FLAG tags at N- and C-terminus, respectively.DepositorAvailable SinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only
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EGFP-Rubicon delta182
Plasmid#28041DepositorAvailable SinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pKM126
Plasmid#105134PurposeClostridium difficile CRISPR-cas9 mutagenesis plasmid Tn916 oriTDepositorInsertsUpstream & Downstream pyrE deletion region
tetR
Cas9
gRNA
UseE. coli - c. difficile shuttle vectorAvailable SinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-K-I-N-CAAX
Plasmid#41640PurposeExpresses caged Cdc42 GEF intersectin: Intersectin Dbl homology (DH) domain fused to wild-type Dronpa145K at C terminus and Dronpa145N at N terminus, for optogenetic activationDepositorInsertDronpa145K-IntersectinDH-Dronpa145N-CAAX
TagsCAAX boxExpressionMammalianMutationK145N on DronpaPromoterCMVAvailable SinceDec. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
hum sgk promoter F4R4
Plasmid#83426Purposepromoter reporter construct to test 5' flanking sequencesDepositorInserthuman Sgk1 gene 5' flanking seq + 117 nt of the 5' UTR (SGK1 Human)
UseLuciferaseTagsLuciferasePromotersgkAvailable SinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
RhoC FLARE.sc mCer, mVenus - wt
Plasmid#65071PurposeRhoC biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoCDepositorInsertRBD, mCerulean, mVenus, RhoC
ExpressionBacterial, Insect, and Mamm…MutationT153M in mVenusPromoterCMV, p10Available SinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGAN230
Plasmid#131122Purposereverse yTRAP, RNQ1-aTF reporting on tetR-mKate2DepositorTypeEmpty backboneExpressionYeastPromoterSUP35Available SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAC025
Plasmid#227597PurposeLentiviral backbone used when cloning pooled prime editing guide RNA libraries already containing a tevopreQ1 RNA motifDepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-memRFP-3xControlgRNA
Plasmid#224567PurposeUbiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter.DepositorInsertmRFP1
UseCRISPRTagsMembrane localization signalMutationCodon optimizedAvailable SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_2FLAG-AKAP79ΔPKA
Plasmid#183452PurposeExpression of human AKAP79 lacking the PKA binding site (Δ391-400)DepositorAvailable SinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
UASGAL-Pgal
Plasmid#60338PurposeContains a promoter of activating sequence derived from native galactose promoter coupled with full length native galactose promoterDepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…ExpressionYeastAvailable SinceNov. 6, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT-TO-N-Flag-8His
Plasmid#157677PurposeN-terminal tagging vectorDepositorInsertFlag-8His
ExpressionMammalianAvailable SinceAug. 4, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDOC-K-glmS-GFPfor
Plasmid#158059PurposeDonor plasmid for insertion of eGFP and the constitutive acpP promoter into the S. Typhimurium chromosome. GFP is in the forward orientation relative to the kanamycin resistance selectable markerDepositorInsertGreen Fluorescent Protein optimised for excitation with UV light
UseSynthetic BiologyExpressionBacterialPromoteracpPAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-3xGFP11-mem-3xControlgRNA
Plasmid#224583PurposeUbiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.DepositorInsert3xGFP11
UseCRISPRTagsMembrane Localization SignalMutationCodon optimizedAvailable SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cry2L348F(1-531)-mCh-BAXS184E
Plasmid#117239PurposeLight activated recruitment of BAX in mammalian cells with Cry2/CIB optogenetic switchDepositorInsertCry2531.L348F-mCh-BAXS184E
ExpressionMammalianAvailable SinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
mc2 mNeon EF1a
Plasmid#206224PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionMammalianAvailable SinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-scrCdh20.1 GFP
Plasmid#209106PurposeGFP expressing scramble of shRNA targeting Cdh20DepositorInsertscrCdh20.1
Available SinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFosill-1
Plasmid#89695Purposeto make Fosmids that can be converted into Illumina sequencable librariesDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
OSCA2.3
Plasmid#136595PurposeExpresses the mechanosensitive channel OSCA2.3 in mammalian cellsDepositorAvailable SinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CD81-(TEV)-tTA
Plasmid#162598Purposehuman CD81-TEV cleavage site-tTADepositorInserthuman CD81 (CD81 Human)
TagsTEV protease cleavage site and tTAExpressionMammalianPromoterCMV promoterAvailable SinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only