We narrowed to 11,748 results for: pcDNA

1. pcDNA3-AMPKsub-YPet-Kras

   Plasmid

   #84638

   Purpose

   Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); plasma membrane targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

   Depositor

   Jin Zhang

   Article

   Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

   Insert

   AMPKsub-YPet-Kras
   

   Use

   Tags

   6xHis, C-terminal targeting sequence from Kras, T…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCDNA-FRT-FAK-kinase10

   Plasmid

   #59123

   Purpose

   FAK fret control sensor without FERM domain with 10 nm linker

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

   Insert

   Focal Adhesion Kinase
   

   Use

   Tags

   FLAG, mCerulean, and mCitrine

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. 835 pcDNA3 HA PTEN G129R

   Plasmid

   #10929

   Depositor

   William Sellers

   Insert

   PTEN (PTEN Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   G129R

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pcDNAM-human eIF2C1-WT-Myc

   Plasmid

   #50360

   Purpose

   Expression of human eIF2C1 in mamalian cells

   Depositor

   Kumiko Ui-Tei

   Article

   Doi et al Curr Biol. 2003 Jan 8;13(1):41-6.

   Insert

   eIF2C1 (AGO1 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA4/TO-mu24-ORF24-2xStrep

   Plasmid

   #138453

   Purpose

   Expresses mu24-ORF24 chimera with a C-terminal 2xStrep tag in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

   Insert

   mu24-ORF24 (ORF24 MHV68/KSHV)
   

   Use

   Tags

   Strep

   Expression

   Mammalian

   Mutation

   mu24 amino acids 1-191 fused to ORF24 amino acids…

   Promoter

   CMV

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA-h-mmPtch1-B-NNQ-HA-GFP

   Plasmid

   #121164

   Purpose

   Expresses mmPtch1-B NNQ variant

   Depositor

   Philip Beachy

   Article

   Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

   Insert

   Ptch1 (Ptch1 Mouse)
   

   Use

   Tags

   GFP and HA

   Expression

   Mammalian

   Mutation

   deleted amino acids 620-643, truncated at 1291, N…

   Promoter

   CMV

   Available since

   Jan. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA-h-mmPtch1-B-I766FV111FL114F-HA-GFP

   Plasmid

   #120909

   Purpose

   Expresses Ptch1-B (I766FV111FL114F)

   Depositor

   Philip Beachy

   Article

   Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

   Insert

   Ptch1 (Ptch1 Mouse)
   

   Use

   Tags

   GFP and HA

   Expression

   Mammalian

   Mutation

   deleted amino acids 620-643, truncated at 1291

   Promoter

   CMV

   Available since

   Jan. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA5/FRT/TO HIS DNAJB2b

   Plasmid

   #19551

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

   Insert

   DNAJB2b
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 7, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-FLAG-Fos Y345A

   Plasmid

   #8970

   Depositor

   John Blenis

   Article

   Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

   Insert

   c-Fos (Fos Rat)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Y345A

   Promoter

   Available since

   Sept. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3-FLAG-RIPK3 4KR

    Plasmid

    #78821

    Purpose

    To overexpress RIPK3 4KR in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK3 (RIPK3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    K55R, K89R, K363R, K501R

    Promoter

    CMV

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1(+)-TTF-1-Q50K-Y54M

    Plasmid

    #75264

    Purpose

    Expresses a double point mutant of human TTF-1

    Depositor

    David Mu

    Article

    Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

    Insert

    TTF-1 (NKX2-1 Human)
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    Q50K and Y54M in the homeodomain

    Promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA-Dest53-SHP-1 S591D

    Plasmid

    #21803

    Depositor

    Stephen Shaw

    Article

    Liu et al J Leukoc Biol. 2007 Sep . 82(3):742-51.

    Insert

    SHP-1 (PTPN6 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    changed Serine 591 to Aspartic acid

    Promoter

    Available since

    Aug. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3.1-mCherry-CyrCR1.miR17

    Plasmid

    #128748

    Purpose

    Expresses mCherry in mammalian cells. ~200 nt of Cyrano with miR-7 site replaced by miR-17 site inserted in mCherry 3'UTR.

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    Cyrano conserved region (CR1) with miR-17 site replacing miR-7 site (OIP5-AS1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA5/FRT/TO-Flag-Tipin

    Plasmid

    #23117

    Depositor

    Aziz Sancar

    Article

    Kemp et al J Biol Chem. 2010 Mar 15. ():.

    Insert

    Tipin (TIPIN Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3-RLuc8C-FHA1-NES

    Plasmid

    #138210

    Purpose

    Encodes PAABD fragment of luminescence-based bimolecular kinase activity reporters (LumKARs); cytosol targeted.

    Depositor

    Jin Zhang

    Article

    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

    Insert

    RLuc8C-FHA1-NES
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNAmyc-hPR-B-486-933

    Plasmid

    #89118

    Purpose

    mammalian expression of human progesterone receptor amino acid residues 486-933 with N-terminal myc tag, has PR DNA and ligand binding domains

    Depositor

    Elizabeth Wilson

    Article

    Liu et al J Biol Chem. 2011 Aug 26;286(34):29951-6

    Insert

    human progesterone receptor ligand binding domain (PGR Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    contains aa 486-933

    Promoter

    CMV

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA5 rtTA BirA eIF4G

    Plasmid

    #158789

    Purpose

    pcDNA5 based transfection plasmid for the exogenous expression of N-terminal BirA-Flag-tagged eIF4G1 lacking the microexon for generation of N2A FlpIn cell lines for BioID

    Depositor

    Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

    Insert

    eIF4G1
    

    Use

    Tags

    3xFlag and BirA*

    Expression

    Mammalian

    Mutation

    Promoter

    miniCMV

    Available since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA5-Flag-Med25-VWA

    Plasmid

    #64772

    Purpose

    Mammalian expression of Flag-tagged Med25-VWA

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

    Insert

    Med25-VWA (MED25 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    C-terminal deletion

    Promoter

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pCDNA3.0_nucLAMA-G97 IRES mYFP

    Plasmid

    #130713

    Purpose

    Plasmid encoding nuclear localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and mYFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    SNAP-Tag-LAMA-G97-NLS IRES mYFP
    

    Use

    Tags

    NLS and SNAP-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA5/FRT/TO HIS DNAK

    Plasmid

    #19544

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

    Insert

    dnaK molecular chaperone DnaK
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge