We narrowed to 18,127 results for: Mut

1. pEGPN-coBCL2L1-F97C

   Plasmid

   #188696

   Purpose

   express codon optimized BCL-XL drug resistant mutant

   Depositor

   Lewis Cantley

   Insert

   coBCL2L1-F97C (BCL2L1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   codon optimized, F97C

   Promoter

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-Hsp25 S15A/S86A

   Plasmid

   #187702

   Purpose

   Mutant Phenotypical Analysis

   Depositor

   Mary Beckerle

   Insert

   Hsp25 (Hspb1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   S15A/S86A

   Promoter

   CMV

   Available Since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAc5.1B-EGFP-DmNot3-F818A-dsRNAres_Q

   Plasmid

   #147262

   Purpose

   Insect Expression of DmNot3-F818A-dsRNAres

   Depositor

   Elisa Izaurralde

   Insert

   DmNot3-F818A-dsRNAres (Not3 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   one silent mutation compared to the sequence give…

   Promoter

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAc5.1B-EGFP-DmSmaug_584-999-V5His6_C

   Plasmid

   #146003

   Purpose

   Insect Expression of DmSmaug_584-999

   Depositor

   Elisa Izaurralde

   Insert

   DmSmaug_584-999 (smg Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   5 silent mutations and a deletion of the AA 961-9…

   Promoter

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAc5.1B-EGFP-DmSmaug_763-999-V5His6_C

   Plasmid

   #146004

   Purpose

   Insect Expression of DmSmaug_763-999

   Depositor

   Elisa Izaurralde

   Insert

   DmSmaug_763-999 (smg Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   2 silent mutations and a deletion of the AA 961-9…

   Promoter

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAc5.1B-EGFP-DmSmaug-A642H-V5His6_C

   Plasmid

   #146005

   Purpose

   Insect Expression of DmSmaug-A642H

   Depositor

   Elisa Izaurralde

   Insert

   DmSmaug-A642H (smg Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   10 silent mutations and a deletion of the AA 961-…

   Promoter

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAc5.1B-EGFP-DmSmaug-K606AR609A-V5His6_C

   Plasmid

   #146007

   Purpose

   Insect Expression of DmSmaug-K606AR609A

   Depositor

   Elisa Izaurralde

   Insert

   DmSmaug-K606AR609A (smg Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   10 silent mutations and a deletion of the AA 961-…

   Promoter

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAc5.1B-lambdaN-HA-DmSmaug_584-999-V5His6_C

   Plasmid

   #146012

   Purpose

   Insect Expression of DmSmaug_584-999

   Depositor

   Elisa Izaurralde

   Insert

   DmSmaug_584-999 (smg Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   5 silent mutations and a deletion of the AA 961-9…

   Promoter

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAc5.1B-lambdaN-HA-DmSmaug-A642H-V5His6_C

   Plasmid

   #146014

   Purpose

   Insect Expression of DmSmaug-A642H

   Depositor

   Elisa Izaurralde

   Insert

   DmSmaug-A642H (smg Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   10 silent mutations and a deletion of the AA 961-…

   Promoter

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAc5.1B-lambdaN-HA-DmSmaug-K606AR609A-V5His6_C

    Plasmid

    #146016

    Purpose

    Insect Expression of DmSmaug-K606AR609A

    Depositor

    Elisa Izaurralde

    Insert

    DmSmaug-K606AR609A (smg Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    10 silent mutations and a deletion of the AA 961-…

    Promoter

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAc5.1B-lambdaN-HA-DmPumilio_B

    Plasmid

    #145945

    Purpose

    Insect Expression of DmPumilio

    Depositor

    Elisa Izaurralde

    Insert

    DmPumilio (pum Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    three silent and one non silent mutation and a de…

    Promoter

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAc5.1B-lambdaN-HA-DmUPF1_92-1180-siRNAres-V5His6_N

    Plasmid

    #146990

    Purpose

    Insect Expression of DmUPF1_92-1180-siRNAres

    Depositor

    Elisa Izaurralde

    Insert

    DmUPF1_92-1180-siRNAres (Upf1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    1 non silent mutations: G995E compared to the seq…

    Promoter

    Available Since

    Feb. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHS29

    Plasmid

    #171205

    Purpose

    bacterial expression for HIS purification of MEG-3 with mutations in the HMG-like motif

    Depositor

    Geraldine Seydoux

    Article

    Schmidt et al bioRxiv 2020.10.15.340570

    Insert

    meg-3 (meg-3 Nematode)
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    F705A,Y708A,Y713A, N725A

    Promoter

    Available Since

    June 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAc5.1B-lambdaN-HA-DmSmaug_763-999-V5His6_C

    Plasmid

    #146013

    Purpose

    Insect Expression of DmSmaug_763-999

    Depositor

    Elisa Izaurralde

    Insert

    DmSmaug_763-999 (smg Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    2 silent mutations and a deletion of the AA 961-9…

    Promoter

    Available Since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-CMV-FLEX-SaCas9-U6-sgVamp1

    Plasmid

    #159919

    Purpose

    Mutagenesis of Vamp1

    Depositor

    Larry Zweifel

    Insert

    Vamp1 (Vamp1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-CMV-FLEX-SaCas9-U6-sgNtn1

    Plasmid

    #159907

    Purpose

    Mutagenesis of Netrin1

    Depositor

    Larry Zweifel

    Insert

    Netrin1 (Ntn1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCSD_SpCas9MT3-FKBP_SL

    Plasmid

    #107302

    Purpose

    Expresses R1335K mutant SpCas9 fused to FKBP in mammalian cells

    Depositor

    Scot Wolfe

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    FKBP

    Expression

    Mammalian

    Mutation

    R1335K and fused to FKBP

    Promoter

    CMV IE94

    Available Since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT7-7 asyn S129W

    Plasmid

    #105744

    Purpose

    Bacterial expression of mutant human alpha synuclein

    Depositor

    Hilal Lashuel

    Insert

    alpha-synuclein S129W (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    S129W

    Promoter

    T7

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT7-7 asyn K32R

    Plasmid

    #105739

    Purpose

    Bacterial expression of mutant human alpha synuclein

    Depositor

    Hilal Lashuel

    Insert

    alpha-synuclein K32R (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    K32R

    Promoter

    T7

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. PTPRJ

    Plasmid

    #38888

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PTPRJ (PTPRJ Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    C1239S inactivating mutation

    Promoter

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

21. pTH672-SUP35-Y351C

    Plasmid

    #29733

    Depositor

    Tobias von der Haar

    Insert

    SUP35 gene encoding translation release factor 3 from S. cerevisiae (SUP35 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Y351C mutant gene of SUP35 including genomic sequ…

    Promoter

    Available Since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. FC511

    Plasmid

    #27330

    Depositor

    Fred Cross

    Article

    Cross et al Yeast. 2011 Feb . 28(2):167-79.

    Insert

    CDC28 (CDC28 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    mutation CDC28-1N (P250L)

    Promoter

    Available Since

    Feb. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

23. Sub2-3 in pRS314

    Plasmid

    #90454

    Purpose

    yeast

    Depositor

    Christine Guthrie

    Article

    Kistler et al Genes Dev. 2001 Jan 1;15(1):42-9.

    Insert

    SUB2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    sub2-3 mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Sub2-4 in pRS314

    Plasmid

    #90455

    Purpose

    yeast

    Depositor

    Christine Guthrie

    Article

    Kistler et al Genes Dev. 2001 Jan 1;15(1):42-9.

    Insert

    SUB2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    sub2-4 mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Sub2-6 in pRS314

    Plasmid

    #90456

    Purpose

    yeast

    Depositor

    Christine Guthrie

    Article

    Kistler et al Genes Dev. 2001 Jan 1;15(1):42-9.

    Insert

    SUB2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    sub2-6 mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLenti6-MK1-EHMT2-V5

    Plasmid

    #31113

    Depositor

    Bernard Futscher

    Insert

    euchromatic histone-lysine N-methyltransferase 2 (EHMT2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Stop codon mutated to Ala

    Promoter

    Available Since

    Aug. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-hSyn-hM4D(Gi)-mCherry

    Plasmid

    #50475

    Purpose

    Gi-coupled hM4D DREADD fused with mCherry under the control of human synapsin promoter.

    Depositor

    Bryan Roth

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    hM4D(Gi)-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    human Synapsin 1

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-hSyn-hChR2(H134R)-EYFP

    Plasmid

    #26973

    Purpose

    AAV expression of humanized ChR2 with H134R mutation fused to EYFP driven by human Synapsin I promoter for optogenetic excitation

    Depositor

    Karl Deisseroth

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, and AAV9

    Insert

    hChR2(H134R)
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    hSyn

    Available Since

    Feb. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-hSyn-DIO-rM3D(Gs)-mCherry

    Plasmid

    #50458

    Purpose

    Double floxed Gs-coupled rM3D DREADD fused with mCherry under the control of human synapsin promoter

    Depositor

    Bryan Roth

    Has Service

    AAV8

    Insert

    rM3D-mCherry
    

    Use

    AAV

    Tags

    HA and mCherry

    Expression

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    human Synapsin 1

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. 7TGC

    Plasmid

    #24304

    Depositor

    Roel Nusse

    Article

    Fuerer et al PLoS One. 2010 . 5(2):e9370.

    Insert

    7xTcf-eGFP // SV40-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    EGFP is GFP with S65T and F64L mutations

    Promoter

    Available Since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pOpen-RNAse A

    Plasmid

    #165565

    Purpose

    Ribonuclease A or RNase A; endoribonuclease purified from bovine pancreas. Important enzyme for the removal of RNA for RNA free DNA purification reactions such as plasmid DNA purification and genomic DNA purification, RNA removal from recombinant protein preparations, ribonuclease protection assays, mapping single-base mutations in DNA/RNA.

    Depositor

    FreeGenes Project, Jennifer Molloy, Drew Endy

    Insert

    RNAse A
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 17, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pUC19_MCM2_dTAG

    Plasmid

    #232731

    Purpose

    Generates MCM2 dTAG C ternimal knock-in cell lines as donor

    Depositor

    Junjie Chen

    Insert

    MCM2 (MCM2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Synonymous mutation

    Promoter

    Available Since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pFLAG_G495R MDA5

    Plasmid

    #216799

    Purpose

    Expresses a single point mutant of melanoma differentiation-associated protein 5

    Depositor

    Sun Hur

    Insert

    melanoma differentiation-associated protein 5 (IFIH1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G495R

    Promoter

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pHMGWA-Pa14Csy4H29A

    Plasmid

    #41092

    Depositor

    Jennifer Doudna

    Article

    Haurwitz et al Science. 2010 Sep 10;329(5997):1355-8.

    Insert

    Csy4
    

    Use

    Tags

    His6-MBP

    Expression

    Mutation

    His29Ala mmutation to abolish ribonuclease activi…

    Promoter

    T7

    Available Since

    Dec. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

35. pLNHA-C1-HseIF4G_O

    Plasmid

    #147087

    Purpose

    Mammalian Expression of HseIF4G

    Depositor

    Elisa Izaurralde

    Insert

    HseIF4G (EIF4G1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one non silent mutation M439V compared to the seq…

    Promoter

    Available Since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCMV-SHP2 CS

    Plasmid

    #8382

    Depositor

    Ben Neel

    Insert

    SHP2 (PTPN11 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C459S, mutation of essential catalytic cysteine

    Promoter

    Available Since

    Sept. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

37. pcDNA3.1-MS2-HA-HsRRP6_Z

    Plasmid

    #148131

    Purpose

    Mammalian Expression of HsRRP6

    Depositor

    Elisa Izaurralde

    Insert

    HsRRP6 (EXOSC10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    2 silent mutations compared to the sequence of is…

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLMB509

    Plasmid

    #40084

    Depositor

    Philip Poole

    Article

    Tett et al Appl Environ Microbiol. 2012 Oct;78(19):

    Inserts

    gfpmut 3.1
    tauR
    tau promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV-CMV-FLEX-SaCas9-U6-sgOprk1

    Plasmid

    #159903

    Purpose

    Mutagenesis of Oprk1

    Depositor

    Larry Zweifel

    Insert

    Oprk1 (Oprk1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pEPkan-S

    Plasmid

    #41017

    Purpose

    PCR template for en passant (two-step Red-mediated) mutagenesis

    Depositor

    Nikolaus Osterrieder

    Article

    Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

    Insert

    Kana_I-SceI
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge