We narrowed to 34,448 results for: CaS;

1. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs7903146-guide3

   Plasmid

   #118200

   Purpose

   CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs7903146 (TCF7L2 GWAS locus)

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK and U6

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs11257655-guide4

   Plasmid

   #118203

   Purpose

   CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus)

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK and U6

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs11257655-guide3

   Plasmid

   #118202

   Purpose

   CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus)

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK and U6

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide3

   Plasmid

   #118160

   Purpose

   CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA3 against the EGFP CDS

   Depositor

   Jorge Ferrer

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a core and U6

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-TALETS3

   Plasmid

   #107301

   Purpose

   Expresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and TALE-VEGFA-TS3 in mammalian cells

   Depositor

   Scot Wolfe

   Insert

   Truncated SpCas9 (aa 574-1368) fused to FKBP and TALE
   

   Use

   CRISPR

   Tags

   FKBP, SV40NLS, 3xHA, c-Myc NLS, and TALE_VEGFA-TS…

   Expression

   Mammalian

   Mutation

   Truncated SpCas9 (aa 574-1368) with R1335K mutati…

   Promoter

   CMV IE94

   Available since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. mei-S332-GFP 5.6kb genomic DNA in Casper4 T331D

   Plasmid

   #112986

   Purpose

   P element vector for transposon insertion of mei-S332-GFP 5.6kb genomic DNA (T331D) into Drosophila genome

   Depositor

   Terry Orr-Weaver

   Article

   Nogueira et al G3 (Bethesda). 2014 Jul 31;4(10):1849-58

   Insert

   mei-S332 (mei-S332 Fly)
   

   Use

   P insertion vector

   Tags

   GFP

   Expression

   Mutation

   T331D

   Promoter

   Available since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. mei-S332-GFP 5.6kb genomic DNA in Casper4 T331A

   Plasmid

   #112988

   Purpose

   P element vector for transposon insertion of mei-S332-GFP 5.6kb genomic DNA (T331A) into Drosophila genome

   Depositor

   Terry Orr-Weaver

   Article

   Nogueira et al G3 (Bethesda). 2014 Jul 31;4(10):1849-58

   Insert

   mei-S332 (mei-S332 Fly)
   

   Use

   P insertion vector

   Tags

   GFP

   Expression

   Mutation

   T331A

   Promoter

   Available since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJSC052 - Bacterial expression plasmid for SpCas9, REC3 FRET variant

   Plasmid

   #101201

   Purpose

   Bacterial expression plasmid for SpCas9, REC3 FRET variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/S701C/S960C
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, S701C and S960C

   Promoter

   T7

   Available since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJSC033 - Bacterial expression plasmid for SpCas9, REC2 FRET variant

   Plasmid

   #101200

   Purpose

   Bacterial expression plasmid for SpCas9, REC2 FRET variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/E60C/D273C
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, E60C and D273C

   Promoter

   T7

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. MMW3689 - human expression plasmid for SpCas9 Cluster 1 Q695 + Q926A

    Plasmid

    #101181

    Purpose

    Human expression plasmid for SpCas9 Cluster 1 Q695 + Q926A variant: CMV-T7-hSpCas9-Cluster1Q695+Q926A(N692A, M694A, H698A, Q926A)-NLS(SV40)-3xFLAG

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster1Q695+Q926A(N692A/M694A/H698A/Q926A)
    

    Use

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    N692A/M694A/H698A/Q926A

    Promoter

    CMV

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. MMW3911 - human expression plasmid for SpCas9 Cluster 1 M694 + Q926A

    Plasmid

    #101182

    Purpose

    Human expression plasmid for SpCas9 Cluster 1 M694 + Q926A variant: CMV-T7-hSpCas9-Cluster 1M694+Q926A(N692A, Q695A, H698A, Q926A)-NLS(SV40)-3xFLAG

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster1M694+Q926A(N692A/Q695A/H698A/Q926A)
    

    Use

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    N692A/Q695A/H698A/Q926A

    Promoter

    CMV

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. MMW3909 - human expression plasmid for SpCas9 Cluster 1 N692 + Q926A

    Plasmid

    #101183

    Purpose

    Human expression plasmid for SpCas9 Cluster 1 N692 + Q926A variant: CMV-T7-hSpCas9-Cluster1N692+Q926A(M694A, Q695A, H698A, Q926A)-NLS(SV40)-3xFLAG

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster1N692+Q926A(M694A/Q695A/H698A/Q926A)
    

    Use

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    M694A/Q695A/H698A/Q926A

    Promoter

    CMV

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. MMW3914 - human expression plasmid for SpCas9 Cluster 1 H698 + Q926A

    Plasmid

    #101180

    Purpose

    Human expression plasmid for SpCas9 Cluster 1 H698 + Q926A variant: CMV-T7-hSpCas9-Cluster1H698+Q926A(N692A, M694A, Q695A, Q926A)-NLS(SV40)-3xFLAG

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster1H698+Q926A(N692A/M694A/Q695A/Q926A)
    

    Use

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    N692A/M694A/Q695A/Q926A

    Promoter

    CMV

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALentry

    Plasmid

    #69233

    Purpose

    R1335K mutant SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites)

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    2X NLS, BbsI TALE entry cassette, and NLS-3XHA-NL…

    Expression

    Mammalian

    Mutation

    Arginine 1335 is changed to Lysine

    Promoter

    Available since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALE_TS3

    Plasmid

    #69231

    Purpose

    Expresses R1335K mutant SpCas9 fused to TALE-TS3 in mammalian cell

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    TS3 TALE
    

    Use

    CRISPR

    Tags

    2X NLS and NLS-3XHA-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLentiRNACRISPR_007 - TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

    Plasmid

    #138149

    Purpose

    Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    CasRx
    

    Use

    Lentiviral

    Tags

    NLS-HA

    Expression

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSLQ9715: pHR-PGK-SV40_NLS-dCasMINI-V4-NFZ-c-Myc_NLS-mCherry-WPRE

    Plasmid

    #203221

    Purpose

    Expression of dCasMINI-V4-NFZ-mCherry in mammalian cells

    Depositor

    Stanley Qi

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCasMINI-V4-NFZ-mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSLQ9926: pHR-PGK-SV40_NLS-dCasMINI-V4-VPR-c- Myc_NLS-mCherry-WPRE

    Plasmid

    #176269

    Purpose

    Expression of dCasMINI-V4-VPR-mCherry in mammalian cells

    Depositor

    Stanley Qi

    Article

    Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

    Insert

    dCasMINI-V4-VPR-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG-enAsCas12a(E174R/S542R/K548R)-NLS(nuc)-3xHA (AAS848)

    Plasmid

    #107941

    Purpose

    Mammalian expression plasmid for human codon optimized enAsCas12a (enhanced AsCas12a) encoding E174R/S542R/K548R substitutions

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized enAsCas12a (E174R/S542R/K548R)
    

    Use

    Tags

    3x HA and NLS (nucleoplasmin)

    Expression

    Mammalian

    Mutation

    E174R, S542R and K548R

    Promoter

    CAG

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSLQ2840 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SadCas9

    Plasmid

    #84246

    Purpose

    Expresses direct fusion VPR-Sa dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    VPR-tagBFP-Sa dCas9
    Puro
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    Nucleoplasmin NLS, SV40 NLS, VPR, and tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and PGK

    Available since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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