We narrowed to 10,056 results for: transfer
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Plasmid#111751PurposeIntermediate vector containing HTT gene, except for polyQ region. Used to clone various polyQ lengths into HTT sequence with N-term FLAG tag. Baculovirus transfer vector for insect and mammalian cellsDepositorTypeEmpty backboneUseBaculovirus expressionTagsFLAGExpressionMammalianAvailable SinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only
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pAAV-Syn-FLEX-rc [ArchT-tdTomato]
Plasmid#123607PurposeAAV mediated expression of ArchT-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. tdTomato has codons varied to reduce recombination. Using bGHpA signal.DepositorInsertArchT-tdTomato
UseAAVTagstdTomatoExpressionMammalianPromoterSynAvailable SinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAVsc.U6-sgFah.Intron9.CMV/CB-EGFP
Plasmid#121509PurposeExpresses sgRNA targeting mouse Fah intron 9 (sgFah.Intron9).DepositorInsertsgFah.intron9
UseAAVExpressionMammalianPromoterU6Available SinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJEP319-pAAV-U6SaCas9gRNA(emx1sg2)-EFS-GFP-KASH-pA
Plasmid#113696PurposeU6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1, followed by an EFS driven GFP-KASH in a separate reading frame.DepositorInsertsSaCas9 gRNA Cassete
GFP
UseAAV and CRISPRTagsKASHAvailable SinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAVsc.U6-sgIdua.CMV/CB-EGFP
Plasmid#121511PurposeExpresses sgRNA targeting mouse Idua intron 9 (sgIdua).DepositorInsertsgIdua
UseAAVExpressionMammalianPromoterU6Available SinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAVsc.U6-sgAspa.CMV/CB-EGFP
Plasmid#121510PurposeExpresses sgRNA targeting mouse Aspa intron 2 (sgAspa).DepositorInsertsgAspa
UseAAVExpressionMammalianPromoterU6Available SinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV Intein-C-SpyCas9, CMV-AcrIIA4-2xmiR-122 target sites
Plasmid#120295PurposeAAV Vector for expression of C-terminal SpyCas9 fragement with split-intein and a CMV-driven AcrIIA4 with two miR-122 binding sitesDepositorInsertC-terminal fragment of SpyCas9
UseAAV and CRISPRTagssplit-inteinExpressionMammalianAvailable SinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJEP318-pAAV-U6SaCas9gRNA(emx1sg1)-EFS-GFP-KASH-pA
Plasmid#113695PurposeU6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1, followed by an EFS driven GFP-KASH in a separate reading frame.DepositorInsertsSaCas9 gRNA Cassete
GFP
UseAAV and CRISPRTagsKASHAvailable SinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEMS2047
Plasmid#79655PurposessAAV genome with Ple258 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPREDepositorInsertssAAV Ple258-EmGFP WPRE
UseAAVPromoterPAX6 EnhancerAvailable SinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEMS2023
Plasmid#49130PurposeAAV plasmid with hs1218 promoter driving expression of iCre.DepositorInsertssAAV-hs1218-iCre
UseAAVExpressionMammalianPromoterhs1218Available SinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEMS2024
Plasmid#49131PurposeAAV plasmid with hs671 promoter driving expression of iCre.DepositorInsertssAAV-hs671-iCre
UseAAVExpressionMammalianPromoterhs671Available SinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEMS2025
Plasmid#49132PurposeAAV plasmid with NOV (Ple303) promoter driving expression of iCre.DepositorInsertssAAV-Ple303-iCre
UseAAVExpressionMammalianPromoterNOVAvailable SinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAVscCBPITuDlet-7Gpluc7xlet7BT
Plasmid#35651DepositorInsertTuD let-7
UseAAVPromoterU6Available SinceMay 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAVf-EnhCB-lacZnls mir-122 1xBS
Plasmid#35643DepositorInsert1 miR-122 target site
UseAAVPromoterCBAvailable SinceApril 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGU06
Plasmid#212148PurposePlasmid expressing alanine-glyoxylate aminotransferase, glutamate-pyruvate aminotransferase and tyrosine aminotransferase under constitutive promoter PgyrA control.DepositorInsertExpressionBacterialPromoterPgyrAAvailable SinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJK516
Plasmid#71578PurposeProduces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Gln mutant (AarCH6-E435Q)DepositorInsertsuccinyl-CoA:acetate CoA-transferase
TagsHis6ExpressionBacterialMutationchanges glutamate-435 to glutaminePromoterT7Available SinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK524
Plasmid#71580PurposeProduces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Asn347>Ala mutant (AarCH6-N347A)DepositorInsertsuccinyl-CoA:acetate CoA-transferase
TagsHis6ExpressionBacterialMutationchanges asparagine-347 to alaninePromoterT7Available SinceApril 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK513
Plasmid#71576PurposeProduces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu294>Ala mutant (AarCH6-E294A)DepositorInsertsuccinyl-CoA:acetate CoA-transferase
TagsHis6ExpressionBacterialMutationchanges glutamate-294 to alaninePromoterT7Available SinceApril 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK564
Plasmid#71581PurposeProduces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Ser71>Ala mutant (AarCH6-S71A)DepositorInsertsuccinyl-CoA:acetate CoA-transferase
TagsHis6ExpressionBacterialMutationchanges serine-71 to alaninePromoterT7Available SinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK517
Plasmid#71579PurposeProduces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Asp mutant (AarCH6-E435D)DepositorInsertsuccinyl-CoA:acetate CoA-transferase
TagsHis6ExpressionBacterialMutationchanges glutamate-435 to aspartatePromoterT7Available SinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only