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pT1QciMP

Plasmid

#62216

Purpose

QUAS transgenesis vector

Depositor

Harold Burgess

Publication

Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

Type

Empty backbone

Use

Zebrafish transgenesis

Promoter

QUAS-E1b

Available since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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763 pME mApple

Plasmid

#195960

Purpose

Gateway pME fluorophore

Depositor

Kristen Kwan, Christian Mosimann

Publication

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

mApple

Use

Other

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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457 p3E-2A-EGFPpA

Plasmid

#195967

Purpose

Gateway p3E 2A fluorophore

Depositor

Kristen Kwan, Christian Mosimann

Publication

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

2A-EGFP

Use

Other

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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ZE45 Dvl-2 pCS2P+

Plasmid

#17086

Depositor

Randall Moon

Publication

Waxman et al Development. 2004 Dec. 131(23):5909-21.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Dvl-2 (dvl2 Zebrafish)

Use

Zebrafish expression

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
597 pCSmCherry

Plasmid

#31165

Depositor

Nathan Lawson

Publication

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mCherry

Use

Expression vector

Available since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCSegfpN5ICD

Plasmid

#22467

Depositor

Nathan Lawson

Publication

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Notch3 (notch3 Zebrafish)

Use

Injection construct

Available since

March 8, 2010

Availability

Academic Institutions and Nonprofits only
pTol2-DRv7

Plasmid

#78627

Purpose

GESTALT V7 barcode for zebrafish

Depositor

Jay Shendure

Publication

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

GESTALT lineage tracing target V7

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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1352wUV2 F2bbs kan GCANNNAGGk

Plasmid

#18752

Depositor

Scot Wolfe

Publication

Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

Insert

3 tandem zinc fingers

Expression

Bacterial

Mutation

A Kan cassette (1090bp) is inserted between two b…

Available since

July 11, 2008

Availability

Academic Institutions and Nonprofits only

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fabp10a:CreERT2; cryaa:mCerulean

Plasmid

#230044

Purpose

Inducible Cre expression in Zebrafish hepatocytes

Depositor

Sumeet Pal Singh

Publication

Eski et al Nat Commun. 2025 Jun 6;16(1):5260. doi:

Insert

CreERT2

Use

Cre/Lox; Zebrafish expression

Promoter

fabp10a

Available since

Feb. 11, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pUFlip-floxed2A-KalTA4-; HE1A:BFP -0

Plasmid

#180010

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Publication

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-KalTA4; HE1A: BFP

Use

Synthetic Biology

Available since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCrysβ:ECFP-LexOP:mCherry-HrasG12V

Plasmid

#89887

Purpose

Human HRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

Depositor

Tatjana Sauka-Spengler

Publication

Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

Insert

mCherry-HRasG12V

Use

Unspecified

Tags

mCherry

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pTol2CG2-hsp70I:QFGal4-SV40pA

Plasmid

#155120

Purpose

Tol2 vector for heat-shock inducible expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis marker

Depositor

Bret Pearson

Publication

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

hsp70l:QFGal4 (hsp70l Zebrafish, Budding Yeast, Neurospora crassa)

Use

Zebrafish expression

Promoter

hsp70l

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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tol2-mpx-cxcr4b whim-gfp

Plasmid

#41089

Depositor

Anna Huttenlocher

Publication

Walters et al Blood. 2010 Oct 14;116(15):2803-11. Epub

Insert

CXCR4b-WHIM (cxcr4b Zebrafish)

Tags

GFP

Expression

Bacterial

Mutation

deleted last 19 amino acids of cxcr4b

Promoter

mpx

Available since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pFT1.0

Plasmid

#46138

Purpose

Tol2-based FlExTrap vector. mCherry is in the "even" or "0" reading frame starting from the exon.

Depositor

Wenbiao Chen

Publication

Ni et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

mCherry

Use

Cre/Lox; Mutagenic gene trap for zebrafish

Available since

June 28, 2013

Availability

Academic Institutions and Nonprofits only

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pCS2 you

Plasmid

#11084

Depositor

William Talbot

Publication

Woods et al PLoS Biol. 2005 Mar . 3(3):e66.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

you (scube2 Zebrafish)

Use

Zebrafish

Available since

Dec. 8, 2005

Availability

Academic Institutions and Nonprofits only
rag1 short

Plasmid

#11300

Depositor

Lisa Steiner

Publication

Willett et al Immunogenetics. 1997 . 45(6):394-404.

Insert

rag1 (rag1 Zebrafish)

Expression

Bacterial

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only
pAc-His-zfTpo

Plasmid

#64309

Purpose

Express soluble His-tagged zebrafish thrombopoietin in insect cells

Depositor

Petr Bartunek

Publication

Svoboda et al Blood. 2014 Jul 10;124(2):220-8. doi: 10

Insert

Thrombopoietin (thpo Zebrafish)

Tags

(His)6

Expression

Insect

Mutation

Thrombopoietin starts at AA37 (AA1-36 encode the …

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
fabp10a:H2B-mGreenLantern

Plasmid

#230045

Purpose

Expression of nuclear green fluorescent protein in zebrafish hepatocytes.

Depositor

Sumeet Pal Singh

Publication

Eski et al Nat Commun. 2025 Jun 6;16(1):5260. doi:

Insert

mGreenLantern

Use

Zebrafish expression

Tags

H2B

Promoter

fabp10a

Available since

Feb. 5, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAF020 p3E-2A-mCerulean

Plasmid

#195974

Purpose

Gateway p3E 2A fluorophore

Depositor

Christian Mosimann

Publication

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

2A-mCerulean

Use

Other

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pME minprom_creERT2

Plasmid

#195966

Purpose

Gateway pME CreERT2 ORF

Depositor

Christian Mosimann

Publication

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

mouse beta-globin minimal promoter creERT2

Use

Cre/Lox

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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