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pET28b-PaODH

Plasmid

#110802

Purpose

Expresses PaODH and opine dehydrogenase from Pseudomonas aeruginosa

Depositor

Audrey Lamb

Publication

McFarlane et al Biochemistry. 2017 Nov 14;56(45):5967-59

Insert

Pa4835

Tags

Hexahistidine tag

Expression

Bacterial

Promoter

T7

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pET15TV-L-SaNAS

Plasmid

#110803

Purpose

Expresses SaNAS a nicotianamine synthase from Staphylcoccus aureus

Depositor

Audrey Lamb

Publication

McFarlane et al Biochemistry. 2017 Nov 14;56(45):5967-59

Insert

SAV2469 (SAV2469 )

Tags

Hexahistidine tag

Expression

Bacterial

Promoter

T7

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pBTK550d

Plasmid

#110617

Purpose

BTK assembled broad-host-range plasmid encoding inducible GFP driven by T7 polymerase

Depositor

Jeffrey Barrick

Publication

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Expression

Bacterial

Available since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pET28b-PaNAS

Plasmid

#110801

Purpose

Expresses PaNAS nicotianamine synthase from pseudomonas aeruginosa

Depositor

Audrey Lamb

Publication

McFarlane et al Biochemistry. 2017 Nov 14;56(45):5967-59

Insert

Pa4836

Tags

Hexahistidine tag

Expression

Bacterial

Promoter

T7

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pET15TV-L-SaODH

Plasmid

#110804

Purpose

Expresses SaODH and opine dehydrogenase from Staphylococcus aureus

Depositor

Audrey Lamb

Publication

McFarlane et al Biochemistry. 2017 Nov 14;56(45):5967-59

Insert

SAV2468 (SAV2468 )

Tags

Hexahistidine tag

Expression

Bacterial

Promoter

T7

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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T1-araC-Pbad-D10A/H840A cas9 ABCD-pACYC-BB

Plasmid

#110547

Purpose

CRISPR interference; works in conjunction with an sgRNA expression vector to silence genes in the E. coli chromosome.

Depositor

Ichiro Matsumura

Insert

dCas9

Expression

Bacterial

Mutation

D10A, H840A

Promoter

pBAD

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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EGFP-PKCZdel239

Plasmid

#110513

Purpose

Constitutive Active

Depositor

Bernardo Mainou

Insert

PKCZ (PRKCZ Human)

Tags

EGFP

Expression

Mammalian

Mutation

Deleted amino terminal 239 amino acids

Available since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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IQSEC2 (IQSEC2A-c001)

Plasmid

#110259

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

IQSEC2 (IQSEC2 Human)

Tags

His10-TEV

Expression

Bacterial

Available since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-VQRRA-PGK-Puro

Plasmid

#110858

Purpose

Lentiviral vector for constitutive expression of Cas9-VQRRA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VQRRA

Use

Lentiviral

Tags

FLAG

Mutation

D1135V, R1335Q, T1337R and NLS sequence at the N-…

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-VQRRA-P2A-GFP-PGK-Puro

Plasmid

#110864

Purpose

Lentiviral vector for constitutive expression of Cas9-VQRRA-P2A-GFP in mammalian cells (codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VQRRA

Use

Lentiviral

Tags

FLAG

Mutation

D1135V, R1335Q, T1337R and NLS sequence at the N-…

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pRP(Exp)-CMV> ORF_924bp (Azgp1):dTomato:IRES:Puro

Plasmid

#110831

Purpose

Expresses both zinc alpha-2 glycoprotein (ZAG) and dTomato (dT) reporter, where the fluorescent dT reporter is fused with the ZAG protein.

Depositor

Ande Satyanarayana

Publication

Elattar et al FASEB J. 2018 Mar 23:fj201701465RR. doi:

Insert

Azgp1 (Azgp1 Mouse)

Use

Non-viral gene expression vector

Tags

dTomato

Promoter

CMV

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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BSP188

Plasmid

#110917

Purpose

For cloning by HR in yeast, Universal MosSCI compatible, has unc54 terminator cloned

Depositor

Alexander Mendenhall

Publication

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

unc54 Terminator

Use

Synthetic Biology; Yeast homologous recombination…

Expression

Worm

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pQC V5 MCS IRES G418

Plasmid

#110345

Purpose

γ-Retroviral transfer vector for cloning and expressing your gene of interest. V5 N-Terminal tag, IRES-driven Geneticin selection.

Depositor

Sandra Martha Gomes Dias

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pQC MCS IRES G418

Plasmid

#110344

Purpose

γ-Retroviral transfer vector for cloning and expressing your gene of interest. IRES-driven Geneticin selection.

Depositor

Sandra Martha Gomes Dias

Publication

Adamoski et al Nat Struct Mol Biol. 2023 Dec;30(12):190

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1-TRC.mKO2_shB3GNT5.1

Plasmid

#110325

Purpose

TRCN0000034809 (Target GCCTATGTAATCTCCGGTGAT), silence human B3GNT5 gene and express monomeric Kusabira-Orange2

Depositor

Sandra Martha Gomes Dias

Publication

Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

Insert

B3GNT5 (B3GNT5 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

RNA polymerase III promoter for human U6 snRNA fo…

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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plko-tet-on-puromycin-shTRX1-259

Plasmid

#110920

Purpose

To knockdown Thioredoxin-1 following doxycycline addition

Depositor

Priyamvada Rai

Publication

Samaranayake et al Nat Commun. 2017 Oct 31;8(1):1204. doi:

Insert

TRX1 (TXN Human)

Use

Lentiviral

Promoter

unknown

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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proISG15 C-term. domain (79-165)

Plasmid

#110763

Purpose

Bacterial expression of proISG15 C-term. domain

Depositor

David Komander

Publication

Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

Insert

ISG15 (ISG15 Human)

Tags

N-His6-3C

Expression

Bacterial

Promoter

T7

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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mature ISG15 C-term. domain (79-157)

Plasmid

#110764

Purpose

Bacterial expression of proISG15 C-term. domain

Depositor

David Komander

Publication

Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

Insert

ISG15 (ISG15 Human)

Tags

N-His6-3C

Expression

Bacterial

Promoter

T7

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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ISG15 C-term. Domain deltaC intein/chitin binding domain (79-154)

Plasmid

#110761

Purpose

Bacterial expression of proISG15

Depositor

David Komander

Publication

Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

Insert

ISG15 (ISG15 Human)

Tags

intein/chitin binding domain

Expression

Bacterial

Promoter

T7

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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KG#62

Plasmid

#110874

Purpose

Expresses the C. elegans acy-1 cDNA pan-neuronally

Depositor

Kenneth Miller

Publication

Reynolds et al Genetics. 2005 Feb;169(2):651-70. doi: 1

Inserts

rab-3 promoter
acy-1 cDNA
unc-54 3' control region with 1 intron just upstream and 1 intron in control region

Expression

Bacterial

Available since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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