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MAPK10 gRNA (BRDN0001146535)

Plasmid

#75982

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK10

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK10 (MAPK10 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAPK10 gRNA (BRDN0001148098)

Plasmid

#75983

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK10

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK10 (MAPK10 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Grin1 KI

Plasmid

#131485

Purpose

Endogenous tagging of GluN1: N-terminal (amino acid position: A20)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Grin1, Grin1 Rat, Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pTR-346 pUC19-tNGFR-P2A-IL2RA N

Plasmid

#186065

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

IL2RA (IL2RA Human)

Use

CRISPR

Tags

Expression

Mutation

WT with tNGFR sequence

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag MKK7B2Jnk3a2

Plasmid

#19729

Depositor

Roger Davis

Article

Lei et al Mol Cell Biol. 2002 Jul . 22(13):4929-42

Insert

MKK7B2Jnk3a2 (Map2k7, MAPK10 Human, Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

March 31, 2009

Availability

Academic Institutions and Nonprofits only
pD649-HAsp-KIRREL1-COMP5AP-AviTag-9xHis

Plasmid

#157440

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

KIRREL1 (KIRREL Human)

Use

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Mutation

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLXSN p200 CUX1

Plasmid

#90470

Purpose

Retroviral vector expressing human p200 CUX1 with a Myc and HA tag at the N- and C-terminus, respectively

Depositor

Alain Nepveu

Article

Kedinger et al J Biol Chem. 2009 Oct 2;284(40):27701-11

Insert

CUX1 (CUX1 Human)

Use

Retroviral

Tags

Ha and myc

Expression

Mammalian

Mutation

Promoter

Moloney murine leukemia virus long terminal repeat

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pIG-874_HA-GD2-28z_CAR_IL2RA

Plasmid

#207504

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

IL2RA, HA-GD2-28z_CAR (IL2RA Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-Ataxin-1, 11NQ [N76/3R]

Plasmid

#114559

Purpose

Mammalian Expression Plasmid of anti-Ataxin-1, 11NQ (Mouse). Derived from hybridoma N76/3.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Ataxin-1, 11NQ (Mus musculus) recombinant mouse monoclonal antibody (Atxn1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

Sept. 26, 2018

Availability

Academic Institutions and Nonprofits only

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rag2:YEATS4_CE_pISceI

Plasmid

#223039

Purpose

Express YEATS4 gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Raja H. et al Molecular Cell, 2025, ISSN 1097-2765

Insert

YEATS4 (YEATS4 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Tags

Expression

Mutation

Promoter

Rag2

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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CILK1-R272Q

Plasmid

#214911

Purpose

expression of the mutant variant of CILK1

Depositor

Pavel Krejčí

Article

Paige Taylor et al Hum Mol Genet. 2016 Sep 15;25(18):3998-4

Insert

CILK1 (CILK1 Human)

Use

Tags

Myc-FLAG

Expression

Mammalian

Mutation

R272Q substitution

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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CILK1-E80K

Plasmid

#214910

Purpose

expression of the mutant variant of CILK1

Depositor

Pavel Krejčí

Article

Paige Taylor et al Hum Mol Genet. 2016 Sep 15;25(18):3998-4

Insert

CILK1 (CILK1 Human)

Use

Tags

Myc-FLAG

Expression

Mammalian

Mutation

E80K substitution

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-KIRREL1-Fc(DAPA)-AviTag-6xHis

Plasmid

#156876

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

KIRREL1 (KIRREL Human)

Use

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shAtxn1

Plasmid

#180387

Purpose

Producing AAV that encodes mouse Atxn1 shRNA with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

shAtxn1 (Atxn1 Mouse)

Use

AAV and RNAi

Tags

Expression

Mutation

Promoter

CBA

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-Cstem-Tac(5A)

Plasmid

#162497

Purpose

Expresses GFP tagged Tac mutant in mammalian cells. The five O-glycosylation sites in the stem region are mutated, and the stem region of CD8a is inserted between GFP and Tac(5A).

Depositor

Lei Lu

Article

Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

Insert

CD8a stem region and Tac full length with mutated stem region (IL2RA, CD8A Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

The five threonine residues are mutated into alan…

Promoter

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-Ataxin-1, 11NQ (Mouse) [N76/8R]

Plasmid

#128641

Purpose

Mammalian Expression Plasmid of anti-Ataxin-1, 11NQ (Mouse). Derived from hybridoma N76/8.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Ataxin-1, 11NQ (Mouse) recombinant mouse monoclonal antibody (Atxn1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pLXSN p110 CUX1

Plasmid

#90471

Purpose

Retroviral vector expressing human p110 CUX1 (amino acids 747-1505) with a Myc and HA tag at the N- and C-terminue, respectively

Depositor

Alain Nepveu

Article

Kedinger et al J Biol Chem. 2009 Oct 2;284(40):27701-11

Insert

CUX1 (amino acids 747-1505) (CUX1 Human)

Use

Retroviral

Tags

HA and Myc

Expression

Mammalian

Mutation

Promoter

Moloney murine leukemia virus long terminal repeat

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223-MAPK10

Plasmid

#23649

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAPK10 (MAPK10 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pGL4.23-IL2RA CaRE2 (plus strand)

Plasmid

#91846

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0623)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE2 (plus strand) (IL2RA Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

rs6602425 T>G, T>C at chr10:6117603

Promoter

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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Anti-Ataxin-1, 11NQ [N76/8R-2b]

Plasmid

#206678

Purpose

Mammalian Expression Plasmid of anti-Ataxin-1, 11NQ (Mouse). Derived from hybridoma N76/8-2b.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Ataxin-1, 11NQ (Mus musculus) recombinant Mouse monoclonal antibody (Atxn1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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