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Plasmid#153220PurposeLevel 0 Arabidopsis U6-promoter vectorDepositorInsertArabidopsis U6 snRNA promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXD70LacZ3-Pcsd-LacZ
Plasmid#191619PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), Pcsd promoter-lacZ, E. lenta constitutive promoterDepositorInsertlacZ
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
p5E-Ui4-eSIBR
Plasmid#82586Purpose5'entry vector containing human ubiquitin C promoter followed by intronically-expressed eSIBR cassette for ubiquitous expression, RNAiDepositorInserthuman ubiquitin C promoter followed by intronically expressed eSIBR cassette
UseTagsExpressionMutationPromoterNoneAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXD70LacZ4-PdegV-LacZ
Plasmid#191620PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), PdegV promoter-lacZ, E. lenta constitutive promoterDepositorInsertlacZ
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
brr2-R1107M pRS313
Plasmid#111417Purposebrr2-R1107M (endogenous promoter)DepositorInsertBRR2
UseTagsExpressionYeastMutationR1107M aa mutation (note, not an RP allele)PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
GFP-PKCA D463N
Plasmid#195203PurposeMammalian expression vector for GFP tagged PKCA. Kinase-inactive mutantDepositorInsertPRKCA (PRKCA Human)
UseTagsExpressionMammalianMutationD463NPromoterAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
RNA polymerase beta prime subunit FLAG
Plasmid#102337PurposeTargeting plasmids for replacing the S. elongatus PCC 7942 genomic copy of Synpcc7942_1524 (Beta prime subunit of RNA polymerase) with a C-terminal FLAG tagged variant.DepositorInsertRNAP Beta Prime Subunit with C-terminal FLAG TAG
UseTags3x FLAG with 3X GSGS linkerExpressionMutationPromoterAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDH-METTL14-WT
Plasmid#141112PurposeOverexpression of WT METTL14 in mammalian cells.DepositorInsertHMETTL14 (METTL14 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-human U6-sgRNA-EF1Alpha-puro-T2A-BFP
Plasmid#118594PurposeParental vector for the CRISPRa libraries. Expresses an sgRNA from the human U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoterDepositorInsertsgRNA/Puro-T2A-BFP
UseLentiviralTagsExpressionMammalianMutationPromoterEF1AlphaAvailable sinceJan. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR-myr-AKT-HA
Plasmid#31790DepositorInsertAkt1 (Akt1 Mouse)
UseEntry vectorTagsHA and MyrExpressionMutationPromoterAvailable sinceOct. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTarget: Ptaq-RFP_PlacIq-GFP
Plasmid#192280PurposeRFP expressed under constitutive promoter Ptaq and GFP expressed under constitutive promoter PlaciqDepositorInsertsRFP
GFP
UseTagsExpressionBacterialMutationPromoterPlacIq and PtaqAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
FKBP:FRB-FBAR
Plasmid#223620PurposeTransient expression of human FBP17 with mGFP, FRB, FKBP and TagBFP tags in mammalian cellsDepositorInsertFBP17 (FNBP1 Human)
UseTagsFKBP, TagBFP and mGFP, FRBExpressionMammalianMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-mGFP-Eps15DN
Plasmid#223621PurposeTransient expression of human Eps15 with mGFP tag in mammalian cellsDepositorInsertEps15 (EPS15 Human)
UseTagsmGFPExpressionMammalianMutationEps15 _ 95-295aaPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUN1214 - pL0_pNMT (pro + 5U)
Plasmid#203893PurposeNMT promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.DepositorInsertNMT promoter and 5'UTR
UseTagsExpressionPlantMutationMutation of BpiI and/or BsaI cut sites for MoClo …PromoterAvailable sinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR1a-mCherry-STIM1
Plasmid#114176PurposeGateway entry clone containing mCherry-STIM1DepositorInsertSTIM1 (STIM1 Human)
UseGateway entry cloneTagsmCherry (inserted after signal peptide)ExpressionMutationPromoterAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL4.15_pSOX17
Plasmid#195496PurposeFirefly luciferase promoter expression vector driven only by the promoter element of human SOX17DepositorInsertPromoter region of SOX17 (SOX17 Human)
UseTagsluc2-hPESTExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
dCas9-CBP FL
Plasmid#179550Purposeencodes S. pyogenes dCas9 with c-terminal fusion of full length human CBP driven by EF-1alpha promoterDepositorInsertS. pyogenes dCas9 with c-terminal fusion of full length human CBP (CREBBP Human)
UseLentiviralTagsFlag TagExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEF-1alphaAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
dCas9-CBP core
Plasmid#179543Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoterDepositorInsertS. Pyogenes dCas9 with c-terminal human CBP core (aa 1084-1701) (CREBBP Human)
UseLentiviralTagsFlag TagExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEF-1alphaAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
dCas9-GCN5 FL
Plasmid#179552Purposeencodes S. pyogenes dCas9 with c-terminal fusion of full length human GCN5 driven by EF-1alpha promoterDepositorInsertS. pyogenes dCas9 with c-terminal fusion of full length human GCN5 (KAT2A Human)
UseLentiviralTagsFlag TagExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEF-1alphaAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKP3915
Plasmid#113335PurposeBeta-estradiol inducible expression vector; Z4 promoter-CYC1 terminator; artificial transcription factor under ACT1 promoterDepositorInsertArtificial Transcription Factor (McIsaac et al, 2013)
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only