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pCS2+ Grx1-roCherry

Plasmid

#141068

Purpose

Fluorescent biosensor for 2GSH/GSSG ratio

Depositor

Dmitry Bilan

Article

Shokhina et al Redox Biol. 2019 Feb;21:101071. doi: 10.

Insert

Grx1-roCherry

Expression

Mammalian

Mutation

Changed Alanine 150 to Cysteine, inserted Threon…

Promoter

CMV, SP6

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pPKm-235

Plasmid

#90509

Purpose

pSIN - EF-1alpha - MTS sHO1 - P2A - MTS - Hy2, vector encoding for mitochondrial-tagged Synechococcus sp. Heme Oxigenase-1 (HO1) and phytochromobilin:ferredoxin oxidoreductase (Hy2)

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

mito-sHO1, mito-Hy2

Use

Lentiviral

Available Since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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pblic-neo TRX1

Plasmid

#110919

Purpose

To overexpress Thioredoxin-1

Depositor

Priyamvada Rai

Article

Samaranayake et al Nat Commun. 2017 Oct 31;8(1):1204. doi:

Insert

TRX1 (TXN Human)

Use

Retroviral

Promoter

unknown

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pPKm-232

Plasmid

#90501

Purpose

pSIN - EF-1alpha - MTS tHO1 - P2A - MTS - tPCYA, vector encoding for mitochondrial-tagged Thermosynechococcus elongates Heme Oxigenase-1 (HO1) and Phycocyanobilin:ferredoxin oxireductase, under EF-1alpha promoter

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

mito-tHO1 and mito-tPCYA

Use

Lentiviral

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pPKm-234

Plasmid

#90507

Purpose

pSIN - EF-1alpha - MTS sHO1 - P2A - MTS - sPCYA, vector encoding for mitochondrial-tagged Synechococcus sp. Heme Oxigenase-1 (HO1) and Phycocyanobilin:ferredoxin oxireductase (PcyA), under EF-1alpha promoter

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

mito-sHO1, mito-sPCYA

Use

Lentiviral

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pPKm-240

Plasmid

#90510

Purpose

pSIN - EF-1alpha - Cyto -sFd - P2A - Cyto - sFNR, vector encoding for cytoplasmic-tagged Synechococcus sp. Heme Oxigenase-1 (HO1) and Phycocyanobilin:ferredoxin oxireductase (PcyA), under EF-1alpha promoter

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

cyto-sFD, cyto-sFNR

Use

Lentiviral

Availability

Academic Institutions and Nonprofits only

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pPKm-300

Plasmid

#104626

Purpose

Expresses pSIN - EF-1alpha - MTS - tFd, vector encoding mitochondrial-tagged Thermosynechococcus elongates Ferredoxin (Fd), under EF-1alpha promoter

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

tFd

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1alpha

Availability

Academic Institutions and Nonprofits only

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pJK394

Plasmid

#71703

Purpose

Produces Acetobacter aceti 1023 thioredoxin reductase 1 (AaTrxB1)

Depositor

T. J. Kappock

Insert

thioredoxin reductase 1

Promoter

T7

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pJK645

Plasmid

#71700

Purpose

Produces E. coli thioredoxin (EcTrxA)

Depositor

T. J. Kappock

Insert

thioredoxin

Expression

Bacterial

Promoter

T7

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pJK647

Plasmid

#71701

Purpose

Produces E. coli thioredoxin reductase (EcTrxB)

Depositor

T. J. Kappock

Insert

thioredoxin reductase

Expression

Bacterial

Promoter

T7

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pJK615

Plasmid

#71699

Purpose

Produces E. coli thioredoxin with a C-terminal His6 tag (EcTrxAH6)

Depositor

T. J. Kappock

Insert

thioredoxin

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pJK580

Plasmid

#71694

Purpose

Produces Acetobacter aceti 1023 thioredoxin with a C-terminal His6 tag (AaTrxAH6)

Depositor

T. J. Kappock

Insert

thioredoxin

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pJK561

Plasmid

#72283

Purpose

Produces Acetobacter aceti 1023 thioredoxin with Cys35>Ser mutant (AaTrxA-C35S)

Depositor

T. J. Kappock

Insert

thioredoxin

Expression

Bacterial

Mutation

changes cysteine-35 to serine

Promoter

T7

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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pJK590

Plasmid

#71698

Purpose

Produces Acetobacter aceti 1023 thioredoxin reductase 1 with a C-terminal His6 tag (AaTrxB1H6)

Depositor

T. J. Kappock

Insert

thioredoxin reductase 1

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pJK651

Plasmid

#71702

Purpose

Produces E. coli thioredoxin reductase with a C-terminal His6 tag (EcTrxBH6)

Depositor

T. J. Kappock

Insert

thioredoxin reductase

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pJK581

Plasmid

#71695

Purpose

Produces Acetobacter aceti 1023 thioredoxin with a C-terminal His6 tag and Cys35>Ser mutant (AaTrxAH6-C35S)

Depositor

T. J. Kappock

Insert

thioredoxin

Tags

His6

Expression

Bacterial

Mutation

changes cysteine-35 to serine

Promoter

T7

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pJK558

Plasmid

#71704

Purpose

Produces Acetobacter aceti 1023 thioredoxin reductase 1 with Cys138>Ser mutant (AaTrxB1-C138S)

Depositor

T. J. Kappock

Insert

thioredoxin reductase 1

Expression

Bacterial

Mutation

changes cysteine-138 to serine

Promoter

T7

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pJK587

Plasmid

#71696

Purpose

Produces Acetobacter aceti 1023 thioredoxin reductase 1 with a C-terminal His6 tag and Cys138>Ser mutant (AaTrxB1H6-C138S)

Depositor

T. J. Kappock

Insert

thioredoxin reductase 1

Tags

His6

Expression

Bacterial

Mutation

changes cysteine-138 to serine

Promoter

T7

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pBAD-TrxRFP1

Plasmid

#115327

Purpose

Bacterial expression of TrxRFP1

Depositor

Huiwang Ai

Article

Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038

Insert

TrxRFP1

Tags

His-Tag

Expression

Bacterial

Promoter

pBAD

Available Since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti6.2_mCherry_Grx1_roGFP2

Plasmid

#155045

Purpose

Fluorescent redox reporter

Depositor

Vanessa LaPointe

Insert

Glutaredoxin-1 (GLRX Human)

Use

Lentiviral

Tags

roGFP2

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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