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We narrowed to 1,104 results for: gibson assembly

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Showing: 481 - 500 of 1104 results

  1. pEND-351_Cacnes_dCas9_CRISPRi

    Plasmid
    #225617
    Purpose
    Cutibacterium acnes replicative plasmid with dCas9 for CRISPRi. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)
    Depositor
    Marc Güell
    Article
    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi
    Insert
    dCas9
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225617-plasmid-map-sequence-id-451418

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  2. pZH517

    Plasmid
    #102670
    Purpose
    Tetracycline inducible expression of GFPmut2 using autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Expression
    Bacterial
    Available Since
    Dec. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    102670-plasmid-map-sequence-id-199582

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  3. pZH512

    Plasmid
    #102666
    Purpose
    Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Expression
    Bacterial
    Available Since
    Dec. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    102666-plasmid-map-sequence-id-199387

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  10. pJB75

    Plasmid
    #226320
    Purpose
    E. coli protein expression for protein purification
    Depositor
    David Savage
    Article
    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d
    Insert
    6xHis-(Y to A MR1-6)-strep
    Tags
    6xHis and Strep
    Expression
    Bacterial
    Mutation
    N-terminal domain deleted, C-terminal domain dele…
    Promoter
    T7
    Available Since
    Oct. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    226320-plasmid-map-sequence-id-449984

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  11. pJB65

    Plasmid
    #226319
    Purpose
    E. coli protein expression for protein purification
    Depositor
    David Savage
    Article
    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d
    Insert
    6xHis- (VTG to AAA MR1-6)-strep
    Tags
    6xHis and Strep
    Expression
    Bacterial
    Mutation
    N-terminal domain deleted, C-terminal domain dele…
    Promoter
    T7
    Available Since
    Oct. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    226319-plasmid-map-sequence-id-449981

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  12. pQE80L TriCatcher-RGDSP-MMP

    Plasmid
    #112633
    Purpose
    Two SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site, an MMP-cleavable sequence and a C-terminal SnoopCatcher
    Depositor
    Mark Howarth
    Article
    Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:
    Insert
    TriCatcher-RGDSP-MMP
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Mutation
    central matrix metalloproteinase-cleavable sequen…
    Promoter
    T5
    Available Since
    Sept. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    112633-plasmid-map-sequence-id-222151

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  13. pEND-320_Cacnes_ori

    Plasmid
    #225602
    Purpose
    Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a low-copy pBR322 E. coli ori
    Depositor
    Marc Güell
    Article
    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225602-plasmid-map-sequence-id-451377

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Showing: 481 - 500 of 1104 results