Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 674 results for: intein

Clear keywords
Showing: 481 - 500 of 674 results

  1. pTYB1-MBD2∆C∆GR pc5079

    Plasmid
    #229759
    Purpose
    Bacterial expression plasmid of MBD2∆C∆GR. MBD2 lacking the C-terminus of MBD2: aa 236-414 and the glycine/arginine rich domain: aa 75-96. C-terminal tagged to intein.
    Depositor
    Cristina Cardoso
    Article
    Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk
    Insert
    MBD2 (Mbd2 Mouse)
    Tags
    Intein
    Expression
    Bacterial
    Mutation
    MBD2 lacking the C-terminus of MBD2: aa 236-414 a…
    Promoter
    T7
    Available Since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    229759-plasmid-map-sequence-id-460764

    Enlarge

  2. pET-Impact-T4gp41

    Plasmid
    #228207
    Purpose
    Expression in BL21(DE3) of T4 helicase
    Depositor
    Stephen Benkovic
    Insert
    41 (41 Escherichia phage T4)
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    228207-plasmid-map-sequence-id-453945

    Enlarge

  3. pET-Impact-T4gp59

    Plasmid
    #228206
    Purpose
    Expression in BL21(DE3) of WT T4 helicase loader
    Depositor
    Stephen Benkovic
    Insert
    59 (59 Escherichia phage T4)
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    228206-plasmid-map-sequence-id-453944

    Enlarge

  4. pTXB3-Flag PLK1 T210D

    Plasmid
    #68272
    Purpose
    bacterial expression of Plk1 T210D mutant
    Depositor
    Stefano Ferrari
    Insert
    PLK1 (PLK1 Human)
    Tags
    Flag and Mxe intein/chitin binding domain
    Expression
    Bacterial
    Mutation
    T210D
    Promoter
    T7
    Available Since
    Aug. 11, 2015
    Availability
    Academic Institutions and Nonprofits only

  5. pTYB1-T4uvsX

    Plasmid
    #228213
    Purpose
    Expression in BL21(DE3) of T4 recombinase
    Depositor
    Stephen Benkovic
    Insert
    uvsX (uvsX Escherichia phage T4)
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    228213-plasmid-map-sequence-id-455994

    Enlarge

  6. pTBY1-T4uvsY

    Plasmid
    #228214
    Purpose
    Expression in BL21(DE3) of T4 UvsY-like recombination mediator
    Depositor
    Stephen Benkovic
    Insert
    uvsY (uvsY Escherichia phage T4)
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    228214-plasmid-map-sequence-id-455995

    Enlarge

  7. MT4_C30Y R31W

    Plasmid
    #105712
    Purpose
    Bacterial expression plasmids for production of recombinant metallothionein 3 subisoform with C30Y and R31W substitution
    Depositor
    Artur Krężel
    Insert
    Metallothionein 4 (MT4 Human)
    Tags
    Chiting Binding Domanin-Intein
    Expression
    Bacterial
    Mutation
    This subisoform has a C30Y and R31W substitution
    Available Since
    April 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    105712-plasmid-map-sequence-id-203330

    Enlarge

  8. pTYB1-T4denV

    Plasmid
    #228216
    Purpose
    Expression in BL21(DE3) of T4 endonuclease V N-glycosylase UV repair enzyme
    Depositor
    Stephen Benkovic
    Insert
    denV
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    228216-plasmid-map-sequence-id-455996

    Enlarge

  9. GPD3_KW

    Plasmid
    #224151
    Purpose
    IPTG-inducible expression of C. elegans protein GPD-3 with a chitin tag
    Depositor
    Katherine Walstrom
    Insert
    GPD-3 (gpd-3 Nematode)
    Tags
    intein-CBD
    Expression
    Bacterial
    Available Since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    224151-plasmid-map-sequence-id-444362

    Enlarge

  10. ALH1_KW

    Plasmid
    #224148
    Purpose
    IPTG-inducible expression of C. elegans protein ALH-1 with a chitin tag
    Depositor
    Katherine Walstrom
    Insert
    ALH-1 (alh-1 Nematode)
    Tags
    Ala-intein-CBD
    Expression
    Bacterial
    Available Since
    Aug. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    224148-plasmid-map-sequence-id-444698

    Enlarge

  11. GPD1_SB

    Plasmid
    #224147
    Purpose
    IPTG-inducible expression of C. elegans protein GPD-1 with a chitin tag
    Depositor
    Katherine Walstrom
    Insert
    GPD-1 (gpd-1 Nematode)
    Tags
    Ala-intein-CBD
    Expression
    Bacterial
    Available Since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    224147-plasmid-map-sequence-id-444366

    Enlarge

  12. ADH1_KW

    Plasmid
    #224149
    Purpose
    IPTG-inducible expression of C. elegans protein ADH-1 with a chitin tag
    Depositor
    Katherine Walstrom
    Insert
    ADH-1 (sodh-1 Nematode)
    Tags
    Ala-intein-CBD
    Expression
    Bacterial
    Available Since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    224149-plasmid-map-sequence-id-444365

    Enlarge

  13. pSW031

    Plasmid
    #231144
    Purpose
    Used to express cyclic sortase cW11 (recognizes APXTG motif) from E. coli.
    Depositor
    Philip Cole
    Article
    Whedon et al J Am Chem Soc. 2024 Nov 25. doi: 10.1021
    Insert
    S. aureus Sortase A mutant
    Tags
    Nostoc punctiform split intein C-terminal fragmen…
    Expression
    Bacterial
    Mutation
    P94S, E108Q, S116V, D160N, T164Q, V168M, L169H, D…
    Promoter
    T7
    Available Since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    231144-plasmid-map-sequence-id-460063

    Enlarge

  14. pET-Impact-T4gp43(exo-)

    Plasmid
    #228202
    Purpose
    Expression in BL21(DE3) of 3' to 5' exonuclease-deficient T4 DNA polymerase D219A
    Depositor
    Stephen Benkovic
    Insert
    43
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Mutation
    Mutated aspartate 219 to alanine (D219A), elimina…
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    228202-plasmid-map-sequence-id-453943

    Enlarge

  15. AAV-pCbh-ABE8e-nSpCas9(D10A/A61R)[N-term] (CA19)

    Plasmid
    #197510
    Purpose
    Cbh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-nSpCas9(D10A/A61R) (to be used with SpRY C-term constructs)
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    AAV-[ITR]-pCBh-BPNLS-TadA8e-nSpCas9(D10A/A61R)[N-term]-NpuN-BPNLS-[ITR]
    Use
    AAV and CRISPR
    Tags
    BPNLS and NpuN(intein)-BPNLS
    Mutation
    ABE8e mutations in TadA and nSpRY(D10A/A61R)
    Promoter
    Cbh
    Available Since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197510-plasmid-map-sequence-id-386692

    Enlarge

  16. AAV-pCbh-SpRY[C-term]-gRNAentry (CA20)

    Plasmid
    #197511
    Purpose
    Cbh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpRY and BsmBI entry cassette to clone SpCas9 gRNA spacer
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    AAV-[ITR]-pCbh-BPNLS-NpuC-SpRY[C-term]-BPNLS-gRNA[BsmBI]-pU6-[ITR]
    Use
    AAV and CRISPR
    Tags
    BPNLS and BPNLS-NpuC(intein)
    Mutation
    C-terminal mutations of SpRY(L1111R/D1135L/S1136W…
    Promoter
    Cbh
    Available Since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197511-plasmid-map-sequence-id-386647

    Enlarge

  17. AAV-pCBh-ABE8e-nSpCas9(D10A)[N-term] (HES1468)

    Plasmid
    #197509
    Purpose
    Cbh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-nSpCas9(D10A)
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    AAV-[ITR]-pCBh-BPNLS-TadA8e-nSpCas9(D10A)[N-term]-NpuN-BPNLS-[ITR]
    Use
    AAV and CRISPR
    Tags
    BPNLS and NpuN(intein)-BPNLS
    Mutation
    ABE8e mutations in TadA and nSpCas9(D10A)
    Promoter
    Cbh
    Available Since
    April 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    197509-plasmid-map-sequence-id-391242

    Enlarge

Showing: 481 - 500 of 674 results