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pTrichoGate-1

Plasmid

#160701

Purpose

derivative vector of pCAMBIA1380 containing lacZ bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

lacZ

Use

Synthetic Biology

Mutation

LacZ bordered by BsaI restrction sites

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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LacZ-5BoxB in pcDNA3.1+

Plasmid

#29729

Depositor

Howard Chang

Article

Wang et al Nature. 2011 Apr 7;472(7341):120-4. doi:

Insert

LacZ-5BoxB

Expression

Mammalian

Available Since

May 23, 2011

Availability

Academic Institutions and Nonprofits only
VinTS-tCRMod-GGSGGS7

Plasmid

#111764

Purpose

Expresses vinculin tension sensor comprised of Clover-(GGSGGS)7-mRuby2 module

Depositor

Brenton Hoffman

Article

LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

Insert

Clover-mRuby2-based vinculin tension sensor with (GGSGGS)7 extensible domain

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 human cofilin S3A

Plasmid

#50854

Purpose

expresses constituvely active cofilin in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 S3A (CFL1 Human)

Expression

Mammalian

Mutation

constitutively active, S3A

Promoter

CMV

Available Since

Feb. 4, 2014

Availability

Industry, Academic Institutions, and Nonprofits
VinTS-tCRMod-GGSGGS5

Plasmid

#111763

Purpose

Expresses vinculin tension sensor comprised of Clover-(GGSGGS)5-mRuby2 module

Depositor

Brenton Hoffman

Article

LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

Insert

Clover-mRuby2-based vinculin tension sensor with (GGSGGS)5 extensible domain

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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FLAG-OTUB1 T134R-pcDNA3.1

Plasmid

#118211

Purpose

Expresses FLAG-OTUB1 T134R mutant in mammalian cells

Depositor

Cynthia Wolberger

Article

Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

Insert

OTUB1 (OTUB1 Human)

Tags

3x FLAG

Expression

Mammalian

Mutation

changed threonine 134 to arginine

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pRmHA3-CH2F2-CG9866

Plasmid

#52286

Purpose

Expresses Drosophila Ataxin-7

Depositor

Jerry Workman

Article

Mohan et al Genes Dev. 2014 Feb 1;28(3):259-72. doi:

Insert

Ataxin-7 (CG9866 Fly)

Tags

2xHA 2xFLAG

Expression

Insect

Available Since

April 24, 2014

Availability

Academic Institutions and Nonprofits only

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pQE-nTR

Plasmid

#21812

Depositor

Wafik El-Deiry

Article

Kim et al J Biol Chem. 2004 Sep 17. 279(38):40044-

Insert

pQE-nTR (TNFSF10 Human)

Expression

Bacterial

Mutation

His tag and aa95-113 were removed from pQE-hTR

Available Since

Sept. 14, 2012

Availability

Academic Institutions and Nonprofits only
pCRII-Topo S100a10 in situ probe

Plasmid

#45603

Depositor

Jeffrey Macklis

Article

Arlotta et al Neuron. 2005 Jan 20;45(2):207-21.

Insert

s100a10 in situ probe (S100a10 Mouse)

Use

In situ

Mutation

fragment contains bp# 21-275 of BC025044

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
pJK2067

Plasmid

#171845

Purpose

C. elegans mex-5 promoter (germline) driven dual fluorescent reporter for boxB tethering (eGFP) with boxB negative control (mCherry)

Depositor

Judith Kimble

Article

Aoki et al Nat Commun. 2021 Feb 12;12(1):996. doi:

Insert

his-58, eGFP, mCherry

Tags

N-terminal Tag OLLAS, V5 and C-terminal Tag PEST…

Expression

Worm

Mutation

boxB wild type and hairpin mutant

Promoter

mex-5 promoter

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pTrichoGate-13

Plasmid

#160713

Purpose

For C-terminal tags with Trichoderma optimised Synthetic 3X Hemagglutinin tag (HA) bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

Trichoderma optimised Synthetic 3X Hemagglutinin tag (HA)

Use

Synthetic Biology

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCRII-Topo Crym in situ probe

Plasmid

#45601

Depositor

Jeffrey Macklis

Article

Arlotta et al Neuron. 2005 Jan 20;45(2):207-21.

Insert

Crym in situ probe (Crym Mouse)

Use

In situ

Mutation

fragment contains nt 756-1191 of NM_016669

Available Since

June 3, 2013

Availability

Academic Institutions and Nonprofits only
MCUb-HA-pLYS5

Plasmid

#236790

Purpose

Clone MCUB into pLYS5 (Hygro resistance) with HA tag

Depositor

Dipayan Chaudhuri

Article

Rai et al J Cell Physiol. 2025 Apr;240(4):e70033.

Insert

MCUB (MCUB Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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MCUb-mVenus-pLJM

Plasmid

#236789

Purpose

MCUB tagged with mVenus for FRET / MW change for stoichiometry

Depositor

Dipayan Chaudhuri

Article

Rai et al J Cell Physiol. 2025 Apr;240(4):e70033.

Insert

MCUB (MCUB Human)

Use

Lentiviral

Tags

mVenus

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pTrichoGate-14

Plasmid

#160714

Purpose

For C-terminal tags with Trichoderma optimised Synthetic 3X-c-Myc tag peptide (EQKLISEEDL) bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

Trichoderma optimised Synthetic 3X-c-Myc tag peptide (EQKLISEEDL)

Use

Synthetic Biology

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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pTrichoGate-17

Plasmid

#160717

Purpose

TrpC Terminator bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

tryptophan synthase transcription terminator (TtrpC) bordered by BsaI restriction sites

Use

Synthetic Biology

Mutation

Incorporation of BsaI restriction sites

Available Since

March 3, 2021

Availability

Academic Institutions and Nonprofits only

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pTrichoGate-15

Plasmid

#160715

Purpose

For C-terminal tags with Trichoderma optimised Synthetic 3X-FLAG peptide bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

Trichoderma optimised Synthetic 3X-FLAG peptide

Use

Synthetic Biology

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTrichoGate-3

Plasmid

#160703

Purpose

tef1-alpha promoter from Trichoderma virens bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

tef1-alpha

Use

Synthetic Biology

Mutation

Incorporation of BsaI restriction sites

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pBacPak-FLAG-HIS-Sgf11

Plasmid

#52287

Purpose

Expresses Drosophila Sgf11

Depositor

Jerry Workman

Article

Mohan et al Genes Dev. 2014 Feb 1;28(3):259-72. doi:

Insert

Sgf11 (Sgf11 Fly)

Tags

HIS, FLAG

Expression

Insect

Available Since

May 15, 2014

Availability

Academic Institutions and Nonprofits only

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pBacPak-HIS-FLAG-CG9866

Plasmid

#52290

Purpose

Expresses Drosophila Ataxin-7

Depositor

Jerry Workman

Article

Mohan et al Genes Dev. 2014 Feb 1;28(3):259-72. doi:

Insert

Ataxin-7 (CG9866 Fly)

Tags

HIS FLAG

Expression

Insect

Available Since

April 24, 2014

Availability

Academic Institutions and Nonprofits only