We narrowed to 1,093 results for: pcs2

1. ZE36 Nlk1(C387Y)-pCS2+

   Plasmid

   #17077

   Depositor

   Randall Moon

   Article

   Thorpe et al Development. 2004 Jun;131(12):2899-909.

   Insert

   Nlk1 (nlk1 Zebrafish)
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   C387Y. Inactive mutant of NLK1.

   Promoter

   Available Since

   Feb. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

2. pCS2 Flag Smad3 AAGY

   Plasmid

   #27004

   Depositor

   Joan Massague

   Article

   Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

   Insert

   Smad3 (SMAD3 Human)
   

   Use

   Xenopus/mammalian/avian/zebrafish

   Tags

   Flag

   Expression

   Mutation

   P181A P182A

   Promoter

   Available Since

   Jan. 11, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pCS2+her3WT-P2A-sfGFP

   Plasmid

   #240545

   Purpose

   Wild-type zebrafish Her3 (ortholog to human HES3) in a -2A-sfGFP backbone for visualization

   Depositor

   Genevieve Kendall

   Article

   Kent et al Dev Biol. 2023 Apr;496:1-14. doi: 10.101

   Insert

   her3 (her3 Zebrafish)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCS2-phiC31o (JDW 453)

   Plasmid

   #229842

   Purpose

   A CMV-driven Phi31 recombinase.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   phiC31 codon optimized, PhiC31o
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCS2+ 3xFLAG-FEM1B C186S

   Plasmid

   #230995

   Purpose

   Transient overexpression of mouse FEM1B in mammalian cells. Mutation abolishes substrate binding.

   Depositor

   Michael Rape

   Article

   Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

   Insert

   FEM1B (Fem1b Mouse)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   mutated Cys186 to Ser

   Promoter

   Available Since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCS2+ 3xFLAG-FEM1B L597A

   Plasmid

   #230994

   Purpose

   Transient overexpression of catalytic null mouse FEM1B in mammalian cells. Mutation abolishes interaction with CUL2.

   Depositor

   Michael Rape

   Article

   Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

   Insert

   FEM1B (Fem1b Mouse)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   mutated Leu597 to Ala

   Promoter

   Available Since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCS2+-Zeo-miR-430-G-G mm

   Plasmid

   #221728

   Purpose

   Expression of Zeocin resistance marker followed by miR-430 target site insertion with G-G mismatch at position 6 in zebrafish embryos.

   Depositor

   David Bartel

   Article

   Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

   Insert

   BleoR-miR-430-G-G mm
   

   Use

   Fish expression

   Tags

   Expression

   Mutation

   G-G mismatch of miR-430 site

   Promoter

   simian CMV IE94

   Available Since

   Dec. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCS2+-Zeo-miR-430-10-11 mm

   Plasmid

   #221727

   Purpose

   Expression of Zeocin resistance marker followed by miR-430 target site insertion with mismatches at position 10 and 11 in zebrafish embryos.

   Depositor

   David Bartel

   Article

   Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

   Insert

   BleoR-miR-430-10-11 mm
   

   Use

   Fish expression

   Tags

   Expression

   Mutation

   Mismatch at positions 10 and 11 of miR-430 site

   Promoter

   simian CMV IE94

   Available Since

   Dec. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCS2+-Zeo-miR-430

   Plasmid

   #221726

   Purpose

   Expression of Zeocin resistance marker followed by perfect miR-430 target site insertion in zebrafish embryos.

   Depositor

   David Bartel

   Article

   Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

   Insert

   BleoR-miR-430
   

   Use

   Fish expression

   Tags

   Expression

   Mutation

   Promoter

   simian CMV IE94

   Available Since

   Dec. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCS2+-FLAG3-drN-hsAgo2

    Plasmid

    #221724

    Purpose

    Expression of human Ago2 with N-terminal 3xFLAG tag in zebrafish embryos. Swapped N-domain for zebrafish Ago2 N-domain.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    drN-hsAgo2 (AGO2 Human)
    

    Use

    Fish expression

    Tags

    3xFLAG

    Expression

    Mutation

    Swap of N-domain for zebrafish Ago2 N-domain

    Promoter

    simian CMV IE94

    Available Since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCS2+-FLAG3-hsN-drAgo2

    Plasmid

    #221717

    Purpose

    Expression of zebrafish Ago2 with N-terminal 3xFLAG tag in zebrafish embryos. Swapped N-domain for human Ago2 N-domain.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    hsN-drAgo2 (ago2 Zebrafish)
    

    Use

    Fish expression

    Tags

    3xFLAG

    Expression

    Mutation

    Swap of N-domain for human Ago2 N-domain

    Promoter

    simian CMV IE94

    Available Since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCS2-MfnG-EcYRS-EGFP*

    Plasmid

    #191119

    Purpose

    A plasmid for incorporation of O-Me-Tyr into an EGFP reporter without O-Me-Tyr feeding for zebrafish

    Depositor

    Han Xiao

    Article

    Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

    Insert

    MfnG - P2A - Mutant E. coli TyrRS - T2A - enhanced green fluorescence protein
    

    Use

    Tags

    His tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCS2-HsProm1s1(W795R)-StayGold

    Plasmid

    #210822

    Purpose

    Mammalian overexpression vector for C-terminally StayGold tagged human Prom1s1 (W795R)

    Depositor

    Luke Chao

    Article

    Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

    Insert

    Prominin 1 (PROM1 Human)
    

    Use

    Tags

    StayGold

    Expression

    Mammalian

    Mutation

    Changed Trp-795 to Arg

    Promoter

    CMV

    Available Since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCS2-HsProm1s1-TwinStrep-His

    Plasmid

    #210816

    Purpose

    Mammalian overexpression vector for C-terminally TwinStrep and His tagged human Prom1s1 (WT)

    Depositor

    Luke Chao

    Article

    Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

    Insert

    Prominin 1 (PROM1 Human)
    

    Use

    Tags

    TwinStrep, 10xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCS2-CIB1-mRuby-Rab35

    Plasmid

    #194334

    Purpose

    For use in light-induced Rab35 protein inactivation through the interaction with CRY2

    Depositor

    Heidi Hehnly

    Article

    Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

    Inserts

    CIB1 (CIB1 Mustard Weed)
    Rab35 (RAB35 Human)
    

    Use

    Tags

    mRuby

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available Since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCS2-mCherry-Cenexin-S796A

    Plasmid

    #186434

    Purpose

    Expresses mCherry Cenexin with S796A mutation in mammalian cells and zebrafish embryos

    Depositor

    Heidi Hehnly

    Article

    Aljiboury et al Mol Biol Cell. 2022 Aug 1;33(9):br14. do

    Insert

    Cenexin (ODF2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Changed Serine 796 to Alanine

    Promoter

    CMV IE94

    Available Since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCS2-foxd4l1.1-AB1-myc

    Plasmid

    #185511

    Purpose

    Expression of Xenopus laevis foxd4

    Depositor

    Sally Moody

    Article

    Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

    Insert

    foxd4l1.1
    

    Use

    In vitro transcription

    Tags

    myc

    Expression

    Mutation

    deletion of IDILGE in acidic blob

    Promoter

    Available Since

    July 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCS2-foxd4l1.1-deltaAB-myc

    Plasmid

    #185503

    Purpose

    Expression of Xenopus laevis foxd4

    Depositor

    Sally Moody

    Article

    Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

    Insert

    foxd4l1.1
    

    Use

    In vitro transcription

    Tags

    myc

    Expression

    Mutation

    acidic blob (aa 21-33) deleted

    Promoter

    Available Since

    July 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCS2-foxd4l1.1-GARP-myc

    Plasmid

    #185517

    Purpose

    Expression of Xenopus laevis foxd4

    Depositor

    Sally Moody

    Article

    Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

    Insert

    foxd4l1.1
    

    Use

    In vitro transcription

    Tags

    myc

    Expression

    Mutation

    Replaces Q to P in GARQYNLI

    Promoter

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCS2-foxd4l1.1-GARG-myc

    Plasmid

    #185515

    Purpose

    Expression of Xenopus laevis foxd4

    Depositor

    Sally Moody

    Article

    Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

    Insert

    foxd4l1.1
    

    Use

    In vitro transcription

    Tags

    myc

    Expression

    Mutation

    Replaces Q to G in GARQYNLI

    Promoter

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge