We narrowed to 1,096 results for: pcs2

1. pCS2+HyPer7

   Plasmid

   #136466

   Purpose

   Mammalian expression of non-targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

   Depositor

   Vsevolod Belousov

   Article

   Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

   Insert

   HyPer7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, SP6

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. LRP6-pCS2

   Plasmid

   #27242

   Depositor

   Xi He

   Article

   Tamai et al Nature. 2000 Sep 28. 407(6803):530-5.

   Insert

   low density lipoprotein receptor-related protein 6 (LRP6 Human)
   

   Use

   Xenopus injection

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 10, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS2-wtFAKSPARK2

   Plasmid

   #195263

   Purpose

   express wtFAKSPARK2

   Depositor

   Xiaokun Shu

   Article

   Li et al Nat Chem Biol. 2023 Jun 22. doi: 10.1038

   Insert

   FAKSPARK2 sensor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCS2-mutFAKSPARK2

   Plasmid

   #195264

   Purpose

   express Tyr to Phe mutant FAKSPARK2

   Depositor

   Xiaokun Shu

   Article

   Li et al Nat Chem Biol. 2023 Jun 22. doi: 10.1038

   Insert

   Tyr to Phe mutant FAKSPARK2 sensor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. PCS2+ Cas9

   Plasmid

   #122948

   Purpose

   For in vitro transcription to produce Cas9 mRNA for microinjection into mouse embryos

   Depositor

   Janet Rossant

   Article

   Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

   Insert

   Cas9
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   SP6

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2+8NmCherry

   Plasmid

   #34936

   Depositor

   Amro Hamdoun

   Article

   Gokirmak et al J Biol Chem. 2012 Nov 2.

   Type

   Empty backbone

   Use

   Sea urchin, zebrafish, xenopus

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   SP6

   Available Since

   Nov. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pCS2+-HypocratesCS

   Plasmid

   #161960

   Purpose

   Mammalian expression of non-targeted inactivated control version of genetically encoded biosensor for (pseudo)hypohalous acids and their derivatives

   Depositor

   Dmitry Bilan

   Article

   Kostyuk et al Nat Commun. 2022 Jan 10;13(1):171. doi:

   Insert

   Hypocrates
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed Cysteine 355 to Serine

   Promoter

   CMV, SP6

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCS2-nCas9n

   Plasmid

   #47929

   Purpose

   expression of an optimized Cas9 for genome-editing in zebrafish

   Depositor

   Wenbiao Chen

   Article

   Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

   Insert

   nls-zcas9-nls
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS2+-eGFP

   Plasmid

   #221710

   Purpose

   Expression of eGFP in zebrafish embryos.

   Depositor

   David Bartel

   Article

   Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

   Insert

   eGFP
   

   Use

   Fish expression

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCS2-Cre.zf1

    Plasmid

    #61391

    Purpose

    mRNA synthesis of Cre recombinase codon modified for zebrafish

    Depositor

    Harold Burgess

    Article

    Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

    Insert

    Cre.zf1
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    sp6

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS2-SauCas9

    Plasmid

    #174040

    Purpose

    In vitro transcription of SauCas9 mRNA

    Depositor

    James Gagnon

    Article

    Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

    Insert

    SauCas9
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCS2(+)-zGrad

    Plasmid

    #119716

    Purpose

    Plasmid to generate zGrad mRNA to inactivate proteins in zebrafish

    Depositor

    Holger Knaut

    Article

    Yamaguchi et al Elife. 2019 Feb 8;8. pii: 43125. doi: 10

    Insert

    zGrad
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCS2-mCherry_SNAP_SNAPV_PACT_Active

    Plasmid

    #173072

    Purpose

    Active SNAP tag at the centrosome

    Depositor

    Heidi Hehnly

    Article

    Bucko et al Elife. 2019 Dec 24;8. pii: 52220. doi: 1

    Insert

    mCherry-SNAP-SNAP-Varied-PACT
    

    Use

    Tags

    mCherry-SNAP-SNAP-Varied

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available Since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCS2+ heiCas9

    Plasmid

    #186929

    Purpose

    For in vitro transcription of heiCas9 mRNA - mammalian codon optimized Cas9, N-terminal hei-tag (Myc-flexible linker-optimizedNLS) and C-terminal oNLS

    Depositor

    Joachim Wittbrodt

    Article

    Thumberger et al Elife. 2022 Mar 25;11. pii: 70558. doi:

    Insert

    myc-flexible linker-oNLS-Cas9-oNLS
    

    Use

    CRISPR

    Tags

    myc-flexible linker-optimizedNLS and optimizedNLS

    Expression

    Mammalian

    Mutation

    Promoter

    sp6

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCS2-Cas9

    Plasmid

    #47322

    Purpose

    For in vivo expression of Cas9 or generation of Cas9 mRNA

    Depositor

    Alex Schier

    Article

    Gagnon et al PLoS One. 2014 May 29;9(5):e98186. doi:

    Insert

    Cas9
    

    Use

    CRISPR; Xenopus and zebrafish expression

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available Since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCS2-Gal4ff.zf1

    Plasmid

    #61392

    Purpose

    mRNA synthesis of Gal4ff codon modified for zebrafish

    Depositor

    Harold Burgess

    Article

    Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

    Insert

    Gal4ff.zf1
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    sp6

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCS2-epNTR

    Plasmid

    #62213

    Purpose

    epNTR (zebrafish optimized nitroreductase, T41L, N71S, F124W) in pCS2

    Depositor

    Harold Burgess

    Article

    Tabor et al J Neurophysiol. 2014 Aug 15;112(4):834-4

    Insert

    epNTR
    

    Use

    Mrna synthesis

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCS2+8CeGFP

    Plasmid

    #34952

    Depositor

    Amro Hamdoun

    Article

    Gokirmak et al J Biol Chem. 2012 Nov 2.

    Type

    Empty backbone

    Use

    Sea urchin, zebrafish, xenopus

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available Since

    Nov. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCS2.aBTX

    Plasmid

    #122257

    Purpose

    Express membrane-tethered alpha-bungarotoxin in Danio rerio

    Depositor

    Loren Looger

    Article

    Wan et al Cell. 2019 Oct 3;179(2):355-372.e23. doi

    Insert

    alpha-bungarotoxin
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCS2+hSpCas9

    Plasmid

    #51815

    Purpose

    Encode hSpCas9 for in vitro transcription with Sp6

    Depositor

    Masato Kinoshita

    Article

    Ansai et al Biol Open. 2014 Apr 11;3(5):362-71. doi:

    Insert

    humanized S.pyogenes Cas9
    

    Use

    CRISPR; Medaka, zebrafish

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available Since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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