We narrowed to 1,241 results for: post

1. pFastBac1-NEDD4L_ΔC2 (157)

   Plasmid

   #187720

   Purpose

   Express GST-tagged NEDD4L ΔC2 (157) in insect cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L residues 157-975 (NEDD4L Human)
   

   Use

   Tags

   GST-HRV 3C site

   Expression

   Insect

   Mutation

   deletion of residues 1-156

   Promoter

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFastBacHTA-NEDD4L_FL

   Plasmid

   #187722

   Purpose

   Express His-tagged NEDD4L FL in insect cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L FL (NEDD4L Human)
   

   Use

   Tags

   His-TEV

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28a(+)-NEDD4L_FL

   Plasmid

   #187705

   Purpose

   Express his-tagged NEDD4L FL in bacterial cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L FL (NEDD4L Human)
   

   Use

   Tags

   His-HRV 3C site

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28a(+)-NEDD4L_W4-HECT

   Plasmid

   #187710

   Purpose

   Express his-tagged NEDD4L W4-HECT in bacterial cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L residues 543-975 (NEDD4L Human)
   

   Use

   Tags

   His-HRV 3C site

   Expression

   Bacterial

   Mutation

   deletion of residues 1-542

   Promoter

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGEX6P1-NEDD4L_WW34

   Plasmid

   #187718

   Purpose

   Express GST-tagged NEDD4L WW34 tandem domains in bacterial cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L residues 492-581 (NEDD4L Human)
   

   Use

   Tags

   GST-HRV 3C site

   Expression

   Bacterial

   Mutation

   only residues 492-581

   Promoter

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGEX6P1-NEDD4L_HECT

   Plasmid

   #187719

   Purpose

   Express GST-tagged NEDD4L HECT domain in bacterial cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L residues 594-975 (NEDD4L Human)
   

   Use

   Tags

   GST-HRV 3C site

   Expression

   Bacterial

   Mutation

   deletion of residues 1-593

   Promoter

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Venus2-Y705F/S727A-STAT3

   Plasmid

   #123177

   Purpose

   Expresses Y705F/S727A STAT3 double mutant fused to the 158-238 fragment of Venus in mammalian cells

   Depositor

   Federico Herrera

   Article

   Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

   Insert

   STAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)
   

   Use

   Tags

   Venus2 (aa 158-238) for bimolecular fluorescence …

   Expression

   Mammalian

   Mutation

   Double Y705F and S727A substitutions

   Promoter

   Available Since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Venus1-Y705F/S727A-STAT3

   Plasmid

   #123176

   Purpose

   Expresses Y705F/S727A STAT3 double mutant fused to the 1-157 fragment of Venus in mammalian cells

   Depositor

   Federico Herrera

   Article

   Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

   Insert

   STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
   

   Use

   Tags

   Venus1 (aa 1-158) for bimolecular fluorescence co…

   Expression

   Mammalian

   Mutation

   Double Y705F and S727A substitutions

   Promoter

   Available Since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pX330-U6-Chimeric_BB-CBh-hSpCas9-hGem(1/110)

   Plasmid

   #71707

   Purpose

   A human codon-optimized SpCas9 fused to first 110 amino acids of human GEMININ and chimeric guide RNA expression plasmid

   Depositor

   Giulio Draetta

   Article

   Gutschner et al Cell Rep. 2016 Feb 3. pii: S2211-1247(16

   Insert

   humanized S. pyogenes Cas9 fused to human Geminin (GMNN Human)
   

   Use

   CRISPR

   Tags

   3xFLAG and hGEM(1/110)

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available Since

   Feb. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pInducer-YAP1-S127A/S397A/S94A

    Plasmid

    #213586

    Purpose

    Doxycycline inducible expression of YAP1-S127A/S397A/S94A cDNA in mammalian cells

    Depositor

    Channing Der

    Article

    Edwards et al Cancer Res. 2023 Nov 7. doi: 10.1158/000

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S127A, S397A, S94A

    Promoter

    Available Since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. miniTol2 x EF1a_EKAREN5 + PGK-puro

    Plasmid

    #167817

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN5
    

    Use

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. miniTol2 x EF1a_EKAREN5 + PGK-blast

    Plasmid

    #167818

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN5
    

    Use

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Venus2-DeltaCT-STAT3

    Plasmid

    #123179

    Purpose

    Expresses a STAT3 deletion mutant (without the C-terminus from SH2 domain) fused to the 158-238 fragment of Venus in mammalian cells

    Depositor

    Federico Herrera

    Article

    Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

    Insert

    STAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)
    

    Use

    Tags

    Venus2 (aa 158-238) for bimolecular fluorescence …

    Expression

    Mammalian

    Mutation

    Deletion of the SH2 and TAD domains (C-terminus, …

    Promoter

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Venus1-DeltaCT-STAT3

    Plasmid

    #123178

    Purpose

    Expresses a STAT3 deletion mutant (without the C-terminus from SH2 domain) fused to the 1-157 fragment of Venus in mammalian cells

    Depositor

    Federico Herrera

    Article

    Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

    Insert

    STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
    

    Use

    Tags

    Venus1 (aa 1-158) for bimolecular fluorescence co…

    Expression

    Mammalian

    Mutation

    Deletion of the SH2 and TAD domains (C-terminus, …

    Promoter

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. miniTol2 x EF1a_EKAREN4 + PGK-puro

    Plasmid

    #167824

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN4
    

    Use

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. miniTol2 x EF1a_EKAREN4 + PGK-blast

    Plasmid

    #167825

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN4
    

    Use

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFastBac1-NEDD4L_W2-W3-W4-HECT (349)

    Plasmid

    #187721

    Purpose

    Express GST-tagged NEDD4L W2-W3-W4-HECT (349) in insect cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 349-975 (NEDD4L Human)
    

    Use

    Tags

    GST-HRV 3C site

    Expression

    Insect

    Mutation

    deletion of residues 1-348

    Promoter

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFastBacHTA-NEDD4L_C2-W1-L-W2-HECT

    Plasmid

    #187723

    Purpose

    Express His-tagged NEDD4L C2-W1-L-W2-HECT chimera in insect cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 1-418+582-975 (NEDD4L Human)
    

    Use

    Tags

    His-TEV

    Expression

    Insect

    Mutation

    deletion of residues 419-581

    Promoter

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFastBacHTA-NEDD4L_W1-L-W2-HECT

    Plasmid

    #187724

    Purpose

    Express His-tagged NEDD4L W1-L-W2-HECT chimera in inserct cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 153-418+582-975 (NEDD4L Human)
    

    Use

    Tags

    His-TEV

    Expression

    Insect

    Mutation

    deletion of residues 1-152 and 419-581

    Promoter

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. His-Precission-NEDD4L (381-975)_pcDNA3.1(+)

    Plasmid

    #187725

    Purpose

    Express his-tagged NEDD4L W2-W3-W4-HECT (381) in mammalian cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 381-975 (NEDD4L Human)
    

    Use

    Tags

    His-HRV 3C site

    Expression

    Mammalian

    Mutation

    deletion of residues 1-380

    Promoter

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge