We narrowed to 7,651 results for: CCH

1. pFA6a-pcr1P-natMX6-pcr2P

   Plasmid

   #80620

   Purpose

   to delete part of the mat1-P locus and fix the mating type in natural S. pombe isolates

   Depositor

   Miguel Godinho Ferreira

   Article

   Avelar et al Nat Commun. 2013;4:2235. doi: 10.1038/nc

   Inserts

   PCR1 from mat1-P
   PCR2 from mat1-P
   

   Expression

   Yeast

   Available Since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFA6a-pcr1M-natMX6-pcr2M

   Plasmid

   #80621

   Purpose

   to delete part of the mat1-M locus and fix the mating type in natural S. pombe isolates

   Depositor

   Miguel Godinho Ferreira

   Article

   Avelar et al Nat Commun. 2013;4:2235. doi: 10.1038/nc

   Inserts

   PCR1 from mat1-M
   PCR2 from mat1-M
   

   Expression

   Yeast

   Available Since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCfB2336

   Plasmid

   #67541

   Purpose

   EasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae XII-5 chromosomal location, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

   Type

   Empty backbone

   Use

   Cre/Lox; Integrative

   Expression

   Yeast

   Available Since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. POT5-TDH3p-ycrtYB-ADH1t

   Plasmid

   #65358

   Purpose

   TU for combinatorial construction of ?-carotene pathway

   Depositor

   Junbiao Dai

   Article

   Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

   Insert

   TDH3p-ycrtYB-ADH1t
   

   Use

   Unspecified

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. POT2-TDH3p-ycrtE-ADH1t

   Plasmid

   #65352

   Purpose

   TU for combinatorial construction of ?-carotene pathway

   Depositor

   Junbiao Dai

   Article

   Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

   Insert

   TDH3p-ycrtE-ADH1t
   

   Use

   Unspecified

   Available Since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. YCp33-YDL183c-HA

   Plasmid

   #70164

   Purpose

   yeast expression of YDL183c from its own promoter and in fusion with a triple HA epitope at the C-terminal end

   Depositor

   Karin Nowikovsky

   Article

   Zotova et al J Biol Chem. 2010 May 7;285(19):14399-41

   Insert

   YDL183c
   

   Tags

   3xHA

   Expression

   Yeast

   Promoter

   YDL183c (217 nucleotides upstream of the ATG)

   Available Since

   Feb. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pRS415-Cox15-13xMyc

   Plasmid

   #61721

   Purpose

   yeast Cox15 heme A synthase with C-terminal 13xMyc tag in low-copy yeast expression vector

   Depositor

   Oleh Khalimonchuk

   Article

   Swenson et al J Biol Chem. 2016 Mar 3. pii: jbc.M115.7

   Insert

   Cox15 (COX15 Budding Yeast)
   

   Tags

   13xMyc

   Expression

   Yeast

   Available Since

   Dec. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

8. SpDJ-1 pET15b

   Plasmid

   #60689

   Purpose

   Recombinant protein expression of DJ-1

   Depositor

   Mark Wilson

   Article

   Madzelan et al FEBS J. 2012 Nov;279(22):4111-20. doi: 1

   Insert

   DJ-1 (SPAC22E12.03c Fission Yeast)
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pAW8ENdeI-cyEGFP-6XDSR

   Plasmid

   #49865

   Purpose

   for Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with 6 copies of the core DSR motif into the S. pombe urg1 locus

   Depositor

   Tony Carr

   Article

   Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   yEGFP

   Available Since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAW8ENdeI-cyEGFP-3XDSR

    Plasmid

    #49863

    Purpose

    for Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with 3 copies of the core DSR motif into the S. pombe urg1 locus

    Depositor

    Tony Carr

    Article

    Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    yEGFP

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAW8ENdeI-c3HA-spo5DSR

    Plasmid

    #49872

    Purpose

    for Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locus

    Depositor

    Tony Carr

    Article

    Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    3XHA

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAW8ENdeI-c3HA-6XDSR

    Plasmid

    #49870

    Purpose

    for Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with 6 copies of the core DSR motif into the S. pombe urg1 locus

    Depositor

    Tony Carr

    Article

    Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    3XHA

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAW8ENdeI-c3HA-3XDSR

    Plasmid

    #49868

    Purpose

    for Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with 3 copies of the core DSR motif into the S. pombe urg1 locus

    Depositor

    Tony Carr

    Article

    Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    3XHA

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAW8ENdeI-cyEGFP-8XDSR

    Plasmid

    #49866

    Purpose

    for Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with 8 copies of the core DSR motif into the S. pombe urg1 locus

    Depositor

    Tony Carr

    Article

    Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    yEGFP

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAW8ENdeI-cyEGFP-4XDSR

    Plasmid

    #49864

    Purpose

    for Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with 4 copies of the core DSR motif into the S. pombe urg1 locus

    Depositor

    Tony Carr

    Article

    Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    yEGFP

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAW8ENdeI-c3HA-8XDSR

    Plasmid

    #49871

    Purpose

    for Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with 8 copies of the core DSR motif into the S. pombe urg1 locus

    Depositor

    Tony Carr

    Article

    Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    3XHA

    Available Since

    Dec. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAW8ENdeI-c3HA-4XDSR

    Plasmid

    #49869

    Purpose

    for Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with 4 copies of the core DSR motif into the S. pombe urg1 locus

    Depositor

    Tony Carr

    Article

    Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    3XHA

    Available Since

    Dec. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. sugarless cDNA

    Plasmid

    #42063

    Depositor

    Norbert Perrimon

    Article

    Hacker et al Development. 1997 Sep;124(18):3565-73.

    Insert

    sugarless (sgl Fly)
    

    Available Since

    Feb. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pLG-deltaA

    Plasmid

    #33151

    Depositor

    Michael Rosbash

    Article

    Legrain et al Cell. 1989 May 19;57(4):573-83.

    Insert

    RP51A-synthetic minimal intron (RPS17A Budding Yeast)
    

    Tags

    LacZ

    Expression

    Yeast

    Mutation

    The RP51A sequence was modified by digestion with…

    Available Since

    Dec. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pTOPO-rRNA-5'ETS 923 to 1179

    Plasmid

    #23995

    Depositor

    Susan Baserga

    Article

    Gallagher et al Genes Dev. 2004 Oct 15. 18(20):2506-17.

    Insert

    rRNA 5'ETS ntds 923 to 1179
    

    Use

    Bacterial cloning - topo

    Mutation

    None

    Available Since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only