We narrowed to 23,171 results for: CRISPR

1. ADRBK1 gRNA (BRDN0001145957)

   Plasmid

   #75682

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human ADRBK1

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   ADRBK1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. ADRBK1 gRNA (BRDN0001147663)

   Plasmid

   #75683

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human ADRBK1

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   ADRBK1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. ADRBK1 gRNA (BRDN0001147919)

   Plasmid

   #75684

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human ADRBK1

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   ADRBK1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. ZAK gRNA (BRDN0001146874)

   Plasmid

   #75598

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human ZAK

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   ZAK
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. PRPF4B gRNA (BRDN0001146471)

   Plasmid

   #76430

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PRPF4B

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PRPF4B
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pdCas9-DNMT3A-EGFP

   Plasmid

   #71666

   Purpose

   Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA

   Depositor

   Vlatka Zoldos

   Article

   Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

   Insert

   S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Synthetic, S. pyogenes, Human)
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9

   Promoter

   CBh

   Available Since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pdCas9-DNMT3A-PuroR

   Plasmid

   #71667

   Purpose

   Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA

   Depositor

   Vlatka Zoldos

   Article

   Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

   Insert

   S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A Synthetic, S. pyogenes, Human)
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, and T2A-PuroR

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9

   Promoter

   CBh

   Available Since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pdCas9-DNMT3A-EGFP (ANV)

   Plasmid

   #71685

   Purpose

   Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-EGFP; cloning backbone for sgRNA

   Depositor

   Vlatka Zoldos

   Article

   Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

   Insert

   S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Synthetic, S. pyogenes, Human)
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9; E756A inactiva…

   Promoter

   CBh

   Available Since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHLS-EF1a-FRB-SpCas9-A

   Plasmid

   #138477

   Purpose

   Expresses FRB fused SpCas9 for NanoMEDIC packaging.

   Depositor

   Akitsu Hotta

   Article

   Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

   Insert

   SpCas9, human codon optimized
   

   Use

   CRISPR

   Tags

   3x HA tag and FRB

   Expression

   Mammalian

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pdCas9-DNMT3A-PuroR (ANV)

    Plasmid

    #71684

    Purpose

    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR; cloning backbone for sgRNA

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A Synthetic, S. pyogenes, Human)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-PuroR

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; E756A inactiva…

    Promoter

    CBh

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pdCas9-DNMT3A-PuroR_v2

    Plasmid

    #74407

    Purpose

    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR (v2) for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR_v2 (DNMT3A Synthetic, S. pyogenes, Human)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-PuroR

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9

    Promoter

    CBh

    Available Since

    April 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHL-EF1a-SphcCas9(D10A)-iP-A

    Plasmid

    #60600

    Purpose

    Expresses D10A mutant (nickase) of human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

    Depositor

    Akitsu Hotta

    Article

    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

    Inserts

    CRISPR Cas9 D10A
    puromycin resistance gene
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Changed Asp 10 to Ala, Codon usage optimized for …

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. dCas9-miniTurbo

    Plasmid

    #219822

    Purpose

    EF1α-driven expression of dCas9 fused to miniTurbo with GFP (Adapted from plasmid #153209)

    Depositor

    Philipp Maass

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    miniTurbo

    Expression

    Mammalian

    Mutation

    D10A/H841A

    Promoter

    EF1a

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCW-Tet-Cas9-BFP-PGK-Blast

    Plasmid

    #196720

    Purpose

    Tet-inducible expression of Cas9-T2A-TagBFP

    Depositor

    Bernhard Schmierer

    Insert

    Cas9-T2A-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG-SV40NLS and Nucleoplasmin NLS

    Promoter

    TRE, PGK

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. CROPseq-Guide-EFS-SpCas9-P2A-EGFP

    Plasmid

    #99248

    Purpose

    single-vector system with EGFP reporter for use in CROPseq knockout screens

    Depositor

    Alex Hewitt

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSaCas9_GFP

    Plasmid

    #64709

    Purpose

    Co-expression of human codon-optimized Staphylococcus aureus Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells and other mammalian cells

    Depositor

    Kiran Musunuru

    Insert

    SaCas9-2A-GFP
    

    Use

    CRISPR

    Tags

    2A-GFP and 3xFLAG-SV40 NLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-ABE-P48R

    Plasmid

    #161816

    Purpose

    Expresses ABE-P48R in mammalian cells

    Depositor

    Sangsu Bae

    Insert

    bpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUC19-MAFA-PQR-NLS-tdTomato-P2A-puro-PGK-puro

    Plasmid

    #121174

    Purpose

    Donor template for CRISPR-Cas9 mediated generation of MAFA-NLS-tdTomato reporter cell lines

    Depositor

    Timo Otonkoski

    Insert

    MAFA-PQR-NLS-tdTomato-P2A-Puro-PGK-Puro
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CAG-ABEmax-P2A-EGFP-ires-puro

    Plasmid

    #121170

    Purpose

    CAG promoter driven expression of ABEmax base editor, EGFP and Puromycin resistance.

    Depositor

    Timo Otonkoski

    Insert

    ABEmax-P2A-EGFP-ires-puro
    

    Use

    CRISPR

    Tags

    P2A-EGFP-ires-puro

    Expression

    Mammalian

    Available Since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. Lenti-EF1alpha-dCas9-VP64-MS2-VP64-ZsG

    Plasmid

    #192665

    Purpose

    3rd generation lenti vector encoding dCas9-VP64 with P2A MS2-VP64 and T2A ZsGreen1

    Depositor

    Jeffrey Miner

    Insert

    dCas9-VP64, MS2-VP64, ZsGreen1
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9

    Promoter

    EF1a

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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