We narrowed to 5,570 results for: SPI

1. 8798 GFP-KrasG12C_2B_hygro

   Plasmid

   #64376

   Purpose

   This a retroviral expression plasmid expressing GFP tagged G12C mutant Kras 2B oncogene along with hygro resistance gene

   Depositor

   Georgios Stathopoulos

   Article

   Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

   Insert

   mouse Kras2B (Kras Mouse)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mutation

   changed glycine 12 to cysteine

   Promoter

   Available since

   July 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. 8047_GFP-Kras2B_ires_puro

   Plasmid

   #64371

   Purpose

   This a retroviral expression plasmid expressing GFP tagged wild type Kras 2B oncogene along with puro resistance gene

   Depositor

   Georgios Stathopoulos

   Article

   Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

   Insert

   mouse Kras2B (Kras Mouse)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mutation

   none

   Promoter

   Available since

   July 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. LeGO-iG-CSF2RB(A455D)

   Plasmid

   #125751

   Purpose

   Lentiviral Expression Vektor with CSF2RB-A455D mutant cDNA and IRES eGFP

   Depositor

   Dirk Heckl

   Article

   Labuhn et al Cancer Cell. 2019 Aug 12;36(2):123-138.e

   Insert

   CSF2RB(A455D)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   A455D, codon optimized

   Promoter

   SFFV

   Available since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-T7-ABE8e-SpCas9-HiFi-P2A-EGFP (LM446)

   Plasmid

   #197506

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-HiFi(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8e-nSpCas9-HiFi-BPNLS-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations in TadA and nSpCas9-HiFi(D10A/R69…

   Promoter

   CMV and T7

   Available since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST-N2H-F2-empty vector

   Plasmid

   #125552

   Purpose

   N2H assay vector with nanoluc fragment2 (empty control, no Gateway cloning site, expressing nanoluc fragment2)

   Depositor

   Marc Vidal

   Article

   Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:

   Type

   Empty backbone

   Use

   In vitro expression

   Tags

   NanoLuc fragment2 alone without any attached prot…

   Expression

   Mammalian and Yeast

   Mutation

   Promoter

   synthetic promoter containing yeast, mammalian, a…

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-sACE2v2(732)-IgG1

   Plasmid

   #154105

   Purpose

   Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2 (IgG1-Fc fusion)

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Use

   Tags

   Fc region of human IgG1

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease and neck domains …

   Promoter

   CMV

   Available since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST-N2H-F1-empty vector

   Plasmid

   #125551

   Purpose

   N2H assay vector with nanoluc fragment1 (empty control, no Gateway cloning site, expressing nanoluc fragment1)

   Depositor

   Marc Vidal

   Article

   Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:

   Type

   Empty backbone

   Use

   In vitro expression

   Tags

   NanoLuc fragment1 alone without any attached prot…

   Expression

   Mammalian and Yeast

   Mutation

   Promoter

   synthetic promoter containing yeast, mammalian, a…

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-sACE2v2(732)-8h

   Plasmid

   #154102

   Purpose

   Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2 (8his tag)

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Use

   Tags

   Short GSG linker and 8his tag

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease domain (a.a. M1-D…

   Promoter

   CMV

   Available since

   June 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOTTC1440 - pAAV CMV-IE Nuc-iRFP-2A-Flpo

   Plasmid

   #112684

   Purpose

   An AAV vector that expresses a nuclear-localized iRFP713 reporter and optimized Flp recombinase

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   iRFP713
   

   Use

   AAV

   Tags

   2A-Flpo and Nuclear localization signal

   Expression

   Mutation

   Promoter

   CMV-IE

   Available since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOTTC1179 - pAAV (flox-stop) TH gRNA A EF1a eGFP

    Plasmid

    #113158

    Purpose

    An AAV vector that expresses a Cre-dependent guide RNA targeting rat TH and expresses EGFP reporter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    gRNA for rat TH
    

    Use

    AAV, CRISPR, and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRI001-pGEM-LS-Bar-PgpdA-dLbCas12a-VPR-Ttrpc-LS

    Plasmid

    #140193

    Purpose

    Chromosomal integration of PgpdA-dLbCas12a-VPR-TtrpC. Contains 1kb homology arms for Aspergillus nidulans and the bar gene for glufosinate selection. Filamentous fungi integrative vector.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

    Inserts

    dLbCas12a-VPR
    Bar
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
    

    Use

    CRISPR and Synthetic Biology; Fungal genome integ…

    Tags

    VPR (VP64-p65-Rta), NLS

    Expression

    Mutation

    D832A DNAse deactivated

    Promoter

    PgpdA and PtrpC

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-sACE2v6-Fc(IgA1)

    Plasmid

    #145161

    Purpose

    Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) high affinity variant 6

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Fc region of human IgA1

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBRPy CAGGS-Mbd3-IRES-PURO

    Plasmid

    #52376

    Purpose

    Mammalian expression vector for constitutive expression of Mbd3

    Depositor

    Jacob Hanna

    Article

    Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

    Insert

    Mbd3 (Mbd3 Mouse)
    

    Use

    Tags

    2xFlag (DYKDDK)

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPHAGE-CR-NENF-C-TAP

    Plasmid

    #128511

    Purpose

    Lentiviral constitutive expression of CRISPR-resistant NENF with c-terminal FLAG and HA tag.

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    NENF (NENF Human)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Synonymous silent mutations to F81, Y82, G83 and …

    Promoter

    CMV

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-T7-ABE8e-SpCas9-HF1-P2A-EGFP (LM422)

    Plasmid

    #197505

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-HF1(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-nSpCas9-HF1-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpCas9-HF1(D10A/N497…

    Promoter

    CMV and T7

    Available since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLD-puro-Cc-PARK7WT-VA

    Plasmid

    #115182

    Purpose

    Lentiviral transduction and expression of PARK7WT into any mammalian cell

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    PARK7WT (PARK7 Human)
    

    Use

    Lentiviral

    Tags

    Versatile affinity tag (2XStreptactin II-6XHis-3X…

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOTTC1076 - prRosa26v1 LSL FLAG-SpCas9n NeoR

    Plasmid

    #113162

    Purpose

    A donor plasmid with homologous arms matching rat Rosa26 and expressing a Cre-dependent FLAG-tagged SpCas9n and a NeoR selectable marker

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    SpCas9
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CAG

    Available since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPHAGE-C20orf24- C20orf24-3’UTR-WT

    Plasmid

    #128508

    Purpose

    Lentiviral constitutive expression of C20orf24 under control of its native WT 3'UTR of human C20orf24.

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    RAB5IF (RAB5IF Human)
    

    Use

    Lentiviral

    Tags

    FLAG and HA tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pOTTC1270 - pAAV Rosa26 gRNA A+B EF1a EGFP

    Plasmid

    #113156

    Purpose

    An AAV vector that expresses guide RNAs targeting rat Rosa26 and expresses EGFP reporter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    Two gRNAs for rat Rosa26
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 and hU6

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. TOPO-EXOC3 (-2455 to -1951)

    Plasmid

    #226441

    Purpose

    For subcloning of human EXOC3 promoter (base pairs -2455 to -1951) or for assays using M13 phage

    Depositor

    Glen Borchert

    Article

    DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

    Insert

    EXOC3 (-2455 to -1951) (EXOC3 Human)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    No

    Promoter

    Available since

    July 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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